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Cartilage preserving fluid and utilization method thereof

A technology of preservation solution and cartilage, which is applied in the field of biomedical engineering to achieve the effects of reducing excessive bypass, preventing cell gap expansion, and increasing chondrocyte activity

Inactive Publication Date: 2019-04-05
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the preservation temperature of cartilage tissue preservation solution, the preservation effect of low temperature is far greater than that of normal temperature.

Method used

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  • Cartilage preserving fluid and utilization method thereof
  • Cartilage preserving fluid and utilization method thereof
  • Cartilage preserving fluid and utilization method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Each 1000mL knee joint cartilage preservation solution contains potassium dihydrogen phosphate 20mmol, histidine 80mmol, lacturonic acid 75mmol, sucrose 50mmol, allopurinol 0.8mmol, low molecular weight dextran-40: 45g, NaCl 45mmol, KCl 25mmol, magnesium sulfate 3mmol , penicillin 70U, reduced glutathione 2mmol, adenosine 7mmol, trehalose 5g, catechin 1mL, vitamin E 200μg, chondroitin sulfate 100mg, the balance is deionized water, the pH of the knee joint cartilage preservation solution is 7.32-7.51. Aliquot, seal, and store at 0-4°C.

Embodiment 2

[0038] Each 1000mL knee cartilage preservation solution contains potassium dihydrogen phosphate 30mmol, histidine salt 15mmol, lactobionic acid 95mmol, sucrose 70mmol, allopurinol 1.2mmol, low molecular weight dextran-40: 55g, NaCl 55mmol, KCl 35mmol, sulfuric acid Magnesium 7mmol, penicillin 90U, reduced glutathione 4mmol, adenosine 3mmol, trehalose 2g, catechin 1mL, vitamin E 200μg, chondroitin sulfate 100mg, the balance is deionized water, the pH of the knee joint cartilage preservation solution The value is 7.33-7.53. Aliquot, seal, and store at 0-4°C.

Embodiment 3

[0040] Each 1000mL knee joint cartilage preservation solution contains 25mmol of potassium dihydrogen phosphate, 80mmol of histidine, 80mmol of lacturonic acid, 60mmol of sucrose, 1mmol of allopurinol, 50g of low molecular weight dextran-40, 50mmol of NaCl, 30mmol of KCl, 5mmol of magnesium sulfate , penicillin 80U, reduced glutathione 3mmol, adenosine 5mmol, trehalose 4g, catechin 1mL, vitamin E 200μg, chondroitin sulfate 100mg, the balance is deionized water, the pH of the knee joint cartilage preservation solution is 7.35-7.53. Aliquot, seal, and store at 0-4°C.

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PUM

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Abstract

The invention provides cartilage preserving fluid and a utilization method thereof. The cartilage preserving fluid is prepared from monopotassium phosphate, histidine or histidine salt, lactobionic acid, saccharose, allopurinol, low-molecular dextran-40, NaCl, KCl, magnesium sulfate, penicillin, reduced glutathione, adenosine, trehalose, catechin, vitamin E and chondroitin sulfate, deionized waterserves as a solvent, a pH value is 7.35-7.52, and the cartilage preserving fluid is used for cartilage preservation at a temperature of 0-4 DEG C. The cartilage preserving fluid and the utilization method thereof have advantages that bioactivity of articular cartilage cells can be kept, degenerative changes of articular cartilages can be delayed, and assistances are provided for clinical treatment of large-area articular cartilage defects and cartilage tissue transplantation.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a cartilage preservation solution and a using method thereof. Background technique [0002] Knee articular cartilage tissue is a complex structure, and its biological activity after preservation is affected by many factors. Joint defects caused by sports, trauma, tumors or other etiologies are difficult problems in medical treatment. Many scholars are exploring a simple and safe method for mid- to long-term preservation of cartilage joints, so that the biomechanics of knee cartilage tissue after preservation, the ultrastructural changes of chondrocytes and vascular endothelial cells can achieve better conditions and biological activities. . [0003] Sports injuries and osteoarthritis (a chronic progressive osteoarthropathy characterized by pathological degeneration of articular cartilage and periarticular bone hyperplasia) can easily lead to knee articular carti...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 何晓乐刘军王晓明李旭升葛伟苏慧李榕张华孙阳王燕杨巧巧甄平周胜虎王伟马永海
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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