Moringa leaf extract as well as preparation method and application thereof

Abstract

The invention provides a moringa leaf extract as well as a preparation method and an application thereof and also provides a pharmaceutical composition containing the moringa leaf extract. The moringaleaf extract contains flavone and polyphenol and has good scavenging effects on DPPH radicals and ABTS radicals and can be applied to periodontitis treatment and bone protection independently or as an active ingredient in the pharmaceutical composition. Moringa is a natural edible substance, can reduce toxic and side effects of medicines while treating periodontitis and can be used for a long term.

Description

technical field

[0001] The invention relates to a leaf extract of Moringa oleifera, in particular to an application of the leaf extract of Moringa oleifera in periodontitis. Background technique

[0002] Periodontitis is a chronic infectious disease involving periodontal supporting tissues (gingiva, periodontal ligament, alveolar bone, and cementum), often leading to inflammatory destruction of periodontal supporting tissues. Periodontitis is mainly divided into chronic periodontitis, aggressive periodontitis, periodontitis reflecting systemic diseases, and necrotizing periodontitis.

[0003] Periodontitis is very harmful, and the key to treatment is to inhibit inflammatory destruction and bone resorption. In the prior art, the most commonly used treatment is periodontal basic treatment first, that is, removal of plaque, dental calculus, etc., and systemic or local drug treatment after the basic treatment is completed.

[0004] The most commonly used drugs are nonsteroidal...

Examples

preparation example Construction

[0045] The invention provides a kind of preparation method of leaf extract of Moringa oleifera, described preparation method comprises the following steps:

[0046] (1) pulverize Moringa leaf, use extraction solution to extract Moringa leaf powder;

[0047] (2) After the extraction is finished, the insoluble matter is removed by suction filtration under reduced pressure, the extract is collected, concentrated and dried to obtain the Moringa oleifera leaf extract.

[0048] Wherein, in step (1), the specific surface area can be enlarged after the leaves of Moringa oleifera are pulverized, and the extraction rate can be improved. The Moringa oleifera leaf powder after preferably pulverizing can all pass through 60 mesh sieves.

[0049] The extraction solution is ethanol solution or water. Wherein the volume fraction of ethanol is 0-70%, preferably 50-70%, more flavonoids and polyphenols can be extracted, and the extracted product has better antioxidant capacity.

[0050] When ...

Embodiment 1

[0084] Accurately weigh 1.0 g of Moringa oleifera leaf powder passed through a 60-mesh sieve, vortex and oscillate the Moringa oleifera leaf powder and ethanol solution, and then reflux for extraction. After the extraction is completed, filter under reduced pressure while hot, concentrate and freeze-dry to obtain Moringa oleifera leaf extract.

[0085] The extraction conditions are as follows: the extraction temperature is 50°C, the ratio of solid to liquid is 1:20, pure water, and the extraction time is 2h.

Embodiment 2

[0087] Accurately weigh 1.0 g of Moringa oleifera leaf powder passed through a 60-mesh sieve, vortex and oscillate the Moringa oleifera leaf powder and ethanol solution, and then reflux for extraction. After the extraction is completed, filter under reduced pressure while hot, concentrate and freeze-dry to obtain Moringa oleifera leaf extract.

[0088] The extraction conditions are as follows: the extraction temperature is 50℃, the ratio of solid to liquid is 1:20, the volume fraction of ethanol is 50%, and the extraction time is 2.0h.

technical field

[0001] The invention relates to a leaf extract of Moringa oleifera, in particular to an application of the leaf extract of Moringa oleifera in periodontitis. Background technique

[0002] Periodontitis is a chronic infectious disease involving periodontal supporting tissues (gingiva, periodontal ligament, alveolar bone, and cementum), often leading to inflammatory destruction of periodontal supporting tissues. Periodontitis is mainly divided into chronic periodontitis, aggressive periodontitis, periodontitis reflecting systemic diseases, and necrotizing periodontitis.

[0003] Periodontitis is very harmful, and the key to treatment is to inhibit inflammatory destruction and bone resorption. In the prior art, the most commonly used treatment is periodontal basic treatment first, that is, removal of plaque, dental calculus, etc., and systemic or local drug treatment after the basic treatment is completed.

[0004] The most commonly used drugs are nonsteroidal...

Examples

preparation example Construction

[0045] The invention provides a kind of preparation method of leaf extract of Moringa oleifera, described preparation method comprises the following steps:

[0046] (1) pulverize Moringa leaf, use extraction solution to extract Moringa leaf powder;

[0047] (2) After the extraction is finished, the insoluble matter is removed by suction filtration under reduced pressure, the extract is collected, concentrated and dried to obtain the Moringa oleifera leaf extract.

[0048] Wherein, in step (1), the specific surface area can be enlarged after the leaves of Moringa oleifera are pulverized, and the extraction rate can be improved. The Moringa oleifera leaf powder after preferably pulverizing can all pass through 60 mesh sieves.

[0049] The extraction solution is ethanol solution or water. Wherein the volume fraction of ethanol is 0-70%, preferably 50-70%, more flavonoids and polyphenols can be extracted, and the extracted product has better antioxidant capacity.

[0050] When ...

Embodiment 1

[0084] Accurately weigh 1.0 g of Moringa oleifera leaf powder passed through a 60-mesh sieve, vortex and oscillate the Moringa oleifera leaf powder and ethanol solution, and then reflux for extraction. After the extraction is completed, filter under reduced pressure while hot, concentrate and freeze-dry to obtain Moringa oleifera leaf extract.

[0085] The extraction conditions are as follows: the extraction temperature is 50°C, the ratio of solid to liquid is 1:20, pure water, and the extraction time is 2h.

Embodiment 2

[0087] Accurately weigh 1.0 g of Moringa oleifera leaf powder passed through a 60-mesh sieve, vortex and oscillate the Moringa oleifera leaf powder and ethanol solution, and then reflux for extraction. After the extraction is completed, filter under reduced pressure while hot, concentrate and freeze-dry to obtain Moringa oleifera leaf extract.

[0088] The extraction conditions are as follows: the extraction temperature is 50℃, the ratio of solid to liquid is 1:20, the volume fraction of ethanol is 50%, and the extraction time is 2.0h.

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