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Molecular markers for diagnosing or treating glioma and their application

A technology for glioma and glioma cells, which is applied in the fields of biomedicine and molecular biology, and can solve the problems of short curative effect and poor curative effect.

Active Publication Date: 2019-07-19
山东华链医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that after the clinical application of anti-vascular therapy, there are generally problems of short-term curative effect and poor curative effect

Method used

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  • Molecular markers for diagnosing or treating glioma and their application
  • Molecular markers for diagnosing or treating glioma and their application
  • Molecular markers for diagnosing or treating glioma and their application

Examples

Experimental program
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Embodiment

[0076] 1. Experimental materials

[0077] Human glioma cell lines U251, U87, and A172 were purchased from the Cell Bank of the Chinese Academy of Sciences. The grading standard was grade IV, and they grew adherently. DMEM complete medium was purchased from Gibco, cell culture flasks and culture plates were purchased from Corning, trypsin was purchased from Invitrogen, fetal bovine serum was purchased from Hyclone, double antibodies were purchased from Sigma, and PBS dry powder was purchased from Shanghai Gefan Biotech Co., Ltd. , Trizol was purchased from TAKARA Company, the whole gene expression profile chip and the whole microRNA expression chip were purchased by Shanghai Kangcheng Biological Co., Ltd. and entrusted for detection, the RT-qPCR kit was purchased from TAKARA Company, RIPA lysate, 5×SDS-PAGE loading buffer Solution, Tris-HCL matching gel kit and primary antibody diluent were purchased from Beyontian Company, protease inhibitors, Tris, SDS, glycine, Tween20, Lipo...

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PUM

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Abstract

The invention provides a molecular marker for diagnosing or treating glioma and applications thereof. Experimental studies show that the expression of microRNA-588 in glioma is significantly up-regulated in hypoxia state than in normoxia state. In vitro experiments show that the miR-588 can inhibit the invasive migration of glioma cells and VM formation ability. In order to clarify the molecular mechanism, further experiments find that Robo1 is a direct and functional related target of the miR-588 in glioma. The knocking out of the Robo1 inhibits the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9), thereby inhibiting invasive migration of glioma and VM formation ability. Therefore, the miR-588 and the Robo1 can be used as a molecular marker for diagnosing or treating glioma.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and molecular biology, and specifically relates to molecular markers for diagnosing or treating glioma and applications thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Glioma is the most common malignant intracranial tumor, with an incidence rate of 3-8 / 100,000, accounting for 40-50% of intracranial tumors. The biological characteristics of glioma mainly include high mortality and recurrence rate, strong invasion ability, strong angiogenesis ability and extensive hypoxic state. [0004] Solid tumors such as breast cancer, liver cancer, and glioma grow rapidly and have correspondin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00A61P35/04
CPCA61K45/00A61P35/00A61P35/04C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 李刚薛皓于锐孙中正郭钦栋申杰
Owner 山东华链医疗科技有限公司
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