Method for judging anaerobic digestion process by using carbon stable isotope fractionation ratio

A carbon stable isotope, anaerobic digestion technology, applied in the direction of material separation, analysis materials, measurement devices, etc., can solve the problems of inaccurate monitoring, etc., to achieve the effect of high labor and cost, high application potential, and large application potential.

Inactive Publication Date: 2019-04-12
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

However, the above evaluation system ignores the impact of other metabolic pathways on δ 13 / 12 CH 4 Impact leading to inaccurate monitoring

Method used

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  • Method for judging anaerobic digestion process by using carbon stable isotope fractionation ratio
  • Method for judging anaerobic digestion process by using carbon stable isotope fractionation ratio
  • Method for judging anaerobic digestion process by using carbon stable isotope fractionation ratio

Examples

Experimental program
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Effect test

Embodiment example 1

[0031] This embodiment provides a method for predicting δ in biogas 13 / 12 C. The effect of fillers on the metabolic pathways of methanogenic archaea during anaerobic digestion. The schematic diagram of the isotope mass spectrometer is as follows figure 1 Shown, acetic acid CH 3 CH in COOH 3 + Produce CH under the use of acetic acid-type methanogenic archaea 4 , -COOH produces CO under the metabolism of acetic acid-type methanogenic archaea 2 . In nature, CH 3 +Medium 12 C and 13 The proportion of C is relatively fixed, 99.9% 12 C and 0.01% 13 C, under the utilization of acetic acid methanogenic archaea, 12 C and 13 C metabolizes to CH 4 Medium, CH 4 Converted to CO by gas chromatography 2 , 12 CO 2 with 13 CO 2 The molecular weight is 44 and 45, CO 2 Molecules are realized by high-voltage magnetic field driven by ion source 12 CO 2 with 13 CO 2 Fractionation, the fractionation ratio is fed back to the computer through signal amplification and receiver, and output through differen...

Embodiment example 2

[0049] The evaluation method of this embodiment is basically similar to that of embodiment 1, specifically as Figure 5 As shown, this embodiment is an important test method for evaluating the anaerobic digestion process of materials through the biogas potential experiment. This embodiment uses δ 13 / 12 C to evaluate the method and accurately predict that urea and ammonia nitrogen have an inhibitory effect on the microorganisms involved in the methane production process during the anaerobic digestion of cellulose, such as Figure 4 in Figure 4 Shown in a~4d.

[0050] according to Figure 4 Medium delta 13 / 12 CH 4 We judged that compared with the negative and positive controls, in the process of anaerobic digestion of urea and cellulose, and ammonia nitrogen and cellulose, the δ in biogas 13 / 12 The sharp decrease of C, δ 13 / 12 The decrease in C causes a decrease in the alpha value, indicating that the hydrophilic methanogenesis pathway dominates these two systems, and the δ produced...

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Abstract

The invention relates to a method for judging an anaerobic digestion process by using a carbon stable isotope fractionation ratio in methane. The method for judging the anaerobic digestion process byusing the carbon stable isotope fractionation ratio in the methane includes the following steps of firstly, collection of a biogas; secondly, measurement of delta13/12CH4 and delta 13/12CO2; thirdly,calculation of delta 13 value (permillage); and fourthly, calculation of a ratio alpha value of the delta 13/12CH4 and the delta 13/12CO2. Aiming at the difficult problem that monitoring a methanogenesis process in the anaerobic digestion process is difficult, and combining the support of a large number of molecular biological data at the early stage, the method for judging the anaerobic digestionprocess by using the carbon stable isotope fractionation ratio in the methane finds out a statistical relationship of composition changes of microbial communities and especially the statistical relationship between a fluctuation of methanogenesis archaea community and a delta value and an alpha value, and a method of rapidly reflecting metabolism of the methanogenesis archaea by using the delta value and the alpha value is obtained; and the method of rapidly reflecting the metabolism of the methanogenesis archaea by using the delta value and the alpha value breaks through the technical barriers of traditional molecular biotechnology of time-consumption, labor-consumption, high cost, poor application potentiality and the like, and achieves the rapid reflection of a methanogenesis metabolicpathway in the anaerobic digestion process.

Description

Technical field [0001] The invention relates to a method for judging the anaerobic digestion process by using the stable carbon isotope fractionation ratio of methane in methane, and more specifically relates to a method for judging the methane production process of anaerobic digestion by using the stable carbon isotope fractionation ratio of methane in methane. The field of digestion process monitoring technology. Background technique [0002] Anaerobic digestion technology uses biomass resources to produce biogas is an effective way to achieve clean utilization. In the process of anaerobic digestion, it is known that various types of monitoring parameters cannot directly reflect the dynamic fluctuations of fermenting microorganisms. It is difficult to diagnose and optimize the anaerobic digestion process in depth with the composition of community structure, the transcription level and diversity of functional genes. [0003] As the end of the entire anaerobic digestion process, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/88C12P5/02
CPCC12P5/023G01N30/02G01N30/72G01N30/88G01N2030/8813G01N2030/8868Y02E50/30
Inventor 吕作鹏储云博袁博梁家卓
Owner XUZHOU NORMAL UNIVERSITY
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