76 results about "Methanogenic archaeon" patented technology

A method of converting CO2 gas produced during industrial processes comprising contacting methanogenic archaea with the CO2 gas under suitable conditions to produce methane.

The present application describes selected polynucleotide sequence from the 1.66-megabase pair genome sequence of an autotrophic archaeon, Methanococcus jannaschii, and its 58- and 16-kilobase pair extrachromosomal elements.

The invention discloses a preparation method of a methane dry fermentation compound bacterial preparation, which comprises the following steps: mixing fermentation substrate with an inoculum which is compound bacteria composed of cellulose decomposing bacteria, proteolytic bacteria, fat decomposing bacteria, hydrogen-producing aceogenic bacteria, sulfate reducing bacteria and methanogenic archaea, adjusting the water content and pH of the mixed materials to 70-80% and 7.0 respectively, performing anaerobic fermentation under the condition of 35 DEG C plus or minus 2 DEG C for 60-90 days to obtain methane dry fermentation compound bacterial preparation. The preparation method of the invention can prepare compound bacterial preparation with a relatively complete methane fermentation function, and the total number of microbial cells and methanogenic archaea cells in the bacterial preparation are at least 10*10<10> / g and 1.0*10<8> / g respectively. Using the compound bacterial preparation as an inoculum of methane dry fermentation can reduce the starting time, and the fermentation process is stable and easy to control.

The invention discloses methane dry fermentation compound bacteria, which comprise 25% to 45% of cellulose decomposing bacteria, 9% to 15% of protein decomposing bacteria, 8% to 15% of fat decomposing bacteria, 8% to 12% of hydrogen-producing acetogenic bacteria, 18% to 31% of sulfate reducing bacteria and 2.5% to 9.5% of methanogenic archaea according to bacterium number percentages. The compound bacteria of microorganisms with a complete methane fermentation function are capable of processing methane dry fermentation raw materials with TS content as 20% to 30% and stable in methane production effect and apt to control methane production.

A method for revealing and distinguishing paddy field formic acid utilization type methanogenic archaea in situ by adopting DNA-based stable isotope probing technology comprises the followings steps: (1), collecting a paddy soil sample; (2), performing a micro universe cultivation experiment of <13>C-formic acid; (3), performing centrifugation layering on the microbe total DNA of <13>C-formic acid cultivation soil by using an ultracentrifuge; (4), performing real-time quantitative PCR analysis and finger-print analysis on methanogenic archaea genes in layers of multiple buoyant densities after layering to judge whether the paddy soil contains the formic acid utilization type methanogenic archaea with metabolic activity. Therefore, the method can be used for keenly revealing the paddy field formic acid utilization type methanogenic archaea in situ based on the DNA-based stable isotope probing technology, and has great significance on the understanding of the paddy field nutrient cycling process driven by microbes and the cognition of the ecological functions of paddy field methanogenic archaea functional groups.

The invention discloses a device and a method for producing methane by sludge removal filtrate. The device comprises an anaerobic reactor, wherein the anaerobic reactor is divided into a sludge reaction area, a three-phase separation area and a gas chamber from top to bottom; the sludge reaction area is provided with a conductive carrier layer; a conductive carrier in the conductive carrier layer consists of carbon fibres and carbon black at the mass ratio of (1:1)-(2:1). The carbon fibres and the carbon black are arranged in the anaerobic reactor to serve as the conductive carrier layer, so that electrons are transferred between methanogenic archaea and syntrophism bacteria through electron carriers of hydrogen, methanoic acid or the like, and inter-specific electron transfer can also be performed directly; therefore, more methane producing ways are provided for producing methane bacteria; the conductive carrier layer is fixed to the anaerobic reactor so as not to lose easily along with the outflow of water flow, and the conductive carrier layer can also be in full contact with wastewater so as to improve the methane producing rate; the water penetrating load of the conductive carrier layer is increased by adopting wastewater cycling, and meanwhile, a fluidizing effect of sludge is also improved; therefore, the methane producing efficiency is improved.

Signal peptides and polypeptides from Methanobrevibacter ruminantium, a methanogenic archaea present in ruminants. Methods of using these peptides to permeabilise microbial cells, particularly M. ruminantium strain M1τ (DSM 1093).

A method of converting CO2 gas produced during industrial processes comprising contacting methanogenic archaea with the CO2 gas under suitable conditions to produce methane.

The invention provides a method for production of high-quality biological methane by biotransformation. The method includes the following steps: firstly, hydrogen trophic methanogenic archaea is enriched in a reactor to show a good hydrogen-eating and methane-production capacity; and on the basis, the reactor is operated by a device console, so that the device can prepare the high-quality biological methane under conditions of only controlling the device gas intake quantity, gas intake ratio and rotating speed at medium temperature. Any other nutrient substances or nutrient solutions do not require to be added in the process. An idea is provided for making biological methane enter natural gas infrastructure.

The invention relates to the technical field of microbes, and discloses a method for producing propionic acid by mixed bacteria system fermentation. The method comprises the following steps: carrying out sequencing batch anaerobic fermentation on a fermentation system composed of Methanogenic archaea / propionic-acid-producing bacteria-containing anaerobic sludge and an anaerobic fermentation culture medium, wherein in the fermentation process, the ammonium ion concentration in the fermentation system is enhanced to 1-2 g / L and kept constant, and methane is collected; and after the fermentation finishes, collecting the fermentation liquid, extracting the propionic acid, and adding the residual flora-containing solid into the anaerobic fermentation culture medium for the next fermentation round. The ammonium ion concentration is regulated to control the flora distribution of the Methanogenic archaea / propionic-acid-producing bacteria-containing anaerobic sludge, so that the fermentation environment is beneficial to the proliferation of the propionic-acid-producing bacteria; and the method can obtain a large amount of methane in the process of producing high-concentration high-purity propionic acid, has the advantages of unrestricted material sources and lower input-output ratio, and is beneficial to industrialized implementation.

System and process are provided for fixation of carbon dioxide through fermentation. More specifically, the disclosure includes fermenting carbon dioxide into methane through methanogenic archaea and producing single cell protein nutrient supplement. The disclosure further provides the integration of methanogenic fermentation with additional processes to achieve improved carbon efficiency.

The invention relates to a method for judging an anaerobic digestion process by using a carbon stable isotope fractionation ratio in methane. The method for judging the anaerobic digestion process byusing the carbon stable isotope fractionation ratio in the methane includes the following steps of firstly, collection of a biogas; secondly, measurement of delta13 / 12CH4 and delta 13 / 12CO2; thirdly,calculation of delta 13 value (permillage); and fourthly, calculation of a ratio alpha value of the delta 13 / 12CH4 and the delta 13 / 12CO2. Aiming at the difficult problem that monitoring a methanogenesis process in the anaerobic digestion process is difficult, and combining the support of a large number of molecular biological data at the early stage, the method for judging the anaerobic digestionprocess by using the carbon stable isotope fractionation ratio in the methane finds out a statistical relationship of composition changes of microbial communities and especially the statistical relationship between a fluctuation of methanogenesis archaea community and a delta value and an alpha value, and a method of rapidly reflecting metabolism of the methanogenesis archaea by using the delta value and the alpha value is obtained; and the method of rapidly reflecting the metabolism of the methanogenesis archaea by using the delta value and the alpha value breaks through the technical barriers of traditional molecular biotechnology of time-consumption, labor-consumption, high cost, poor application potentiality and the like, and achieves the rapid reflection of a methanogenesis metabolicpathway in the anaerobic digestion process.

The invention discloses a fast quantitative detection method for methanogenic archaea in oil-water well output liquid. The fast quantitative detection method comprises the following step: 1, compounding specific primers; 2, preparing standard quality grains; 3, establishing a fluorogenic quantitative PCR reaction process and a reaction system; 4, drawing a standard curve; 3, evaluating the method; 6, sampling a field sample, collecting thalli, and extracting DNA; 7, quantitatively detecting methanogenic archaea of the field sample. The method is high in accuracy, strong in specificity, simple in operation, high in repeatability and short in detection time; the process from extraction of sample DNA to the obtaining of the detection result requires less than 3 h; high performance detection can be performed to 96 samples at the same time; the method is used for fast quantitative detection of methanogenic archaea in oil-water well output liquid.

A method improves a symptom or indicator of disease in a patient with chronic lung disease. The patient is identified as likely to respond to treatment by reduction of methane production and / or methanogenic archaea in the digestive tract by evaluating the patient as methane positive. One or more agents is administered to the identified methane positive patient. The agents reduce or eliminate the methane production and / or the methanogenic archaea.

A method of converting CO2 gas produced during industrial processes comprising contacting methanogenic archaea with the CO2 gas under suitable conditions to produce methane.

The invention belongs to the technical field of kitchen waste recycling, and particularly relates to polyaniline magnetic biochar prepared on the basis of biogas residues and application of the polyaniline magnetic biochar in promoting enrichment of anaerobic co-digestion synergistic functional bacteria and methanogenic archaea in an anaerobic co-digestion system. According to the method, a proper amount of phosphate is added into the dry anaerobic digestion system of the kitchen garbage to activate the magnetic enteromorpha hydrothermal carbon so as to optimize the microbial community structure of the digestion system, enhance the microbial activity and enrich functional microorganisms such as clostridium and methane bag-shaped bacteria, so that the dry anaerobic digestion process of the kitchen garbage is effectively promoted, and the digestion efficiency of the kitchen garbage is improved. And the biogas yield is improved. And the method has great significance for realizing reduction, harmlessness and recycling of the kitchen garbage.

The invention discloses methanogenic archaea, and belongs to the technical field of microorganisms. The preservation number of the methanogenic archaea DRTY01 is CCTCC NO: M 2025822, the methanogenic archaea DRTY01 is preserved in the China Center for Type Culture Collection, and the methanogenic archaea DRTY01 is a currently separated first strain of archaea with a methanogenic function, and is a new representative in the archaea family. The methanogenic archaea DRTY01 is also the only strain capable of efficiently utilizing methanol to produce methane at the ultra-high temperature of 85 DEG C at present, so that an efficient, environment-friendly and feasible technical scheme is provided for energy supply in specific scenes such as an ultra-high temperature fermentation tank.

The present invention discloses a method for promoting the anaerobic digestion process of sludge by coupling treatment of oxygen and corn straw biogas residue biochar, belonging to the technical field of solid waste resource treatment. The present invention discloses a method for promoting the anaerobic digestion process of sludge by coupling treatment of oxygen and biogas residue biochar. The method increases the anaerobic hydrolysis and acidification rate of sludge by injecting a trace amount of oxygen, and at the same time utilizes the oxygen-containing functional groups on the surface of biogas residue biochar as a bridge to accelerate the electron transfer between volatile fatty acid oxidizing bacteria and methanogenic archaea, promote the degradation of volatile fatty acids, increase the maximum methane production potential, promote the maximum methane production rate and increase the cumulative methane production, which is conducive to further improving the economic and environmental benefits of anaerobic digestion technology.

The invention relates to the technical field of microorganisms, and in particular relates to a prevotella bruceli B14 capable of improving the milk yield and the milk-fat percentage of ruminants and application of the prevotella bruceli B14. The invention discloses a strain of prevotella bruceli B14, and the Latin name of the prevotella bruceli B14 is prevotella bruceli B14. The preservation number of the strain is CCTCC NO: M 20242730. The prevotella bruceli B14 provided by the invention can increase the milk yield and the milk fat percentage of ruminants by increasing the concentration of total volatile fatty acid, acetic acid, propionic acid and valeric acid in the rumen of the ruminants. In addition, the compound also has the function of synthesizing nicotinic acid, and can activate an mTORC1 signal channel in the mammary gland of the ruminant in a GPR109A-dependent manner and promote the expression of lipid synthesis genes so as to promote milk fat synthesis. In addition, the prevotella buchneri B14 also has the function of reducing the abundance of methanogenic archaea in rumen, so that the prevotella buchneri B14 is expected to improve the energy utilization efficiency by reducing the methane emission of ruminants.

The invention discloses a preparation method of a biogas dry fermentation composite bacterial agent, which uses the composite bacteria composed of cellulolytic bacteria, proteolytic bacteria, lipolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria and methanogenic archaea as Inoculum, mix the inoculum with the fermentation substrate, adjust the moisture content of the mixture to 70-80%, and the pH value to 7.0, carry out anaerobic fermentation at 35°C±2°C, and obtain biogas after 60-90 days of fermentation Dry fermentation compound bacterial agent. The invention can prepare a composite bacterial agent with relatively complete biogas fermentation function microorganisms. The total number of microbial cells in the microbial agent is at least 1.0×1010 / g, and the total number of methanogenic archaeal cells is at least 1.0×108 / g. Using it as an inoculum for biogas dry fermentation can shorten the start-up time, and the fermentation process is stable and easy to control.

System and process are provided for fixation of carbon dioxide through fermentation. More specifically, the disclosure includes fermenting carbon dioxide into methane through methanogenic archaea. The disclosure further provides the integration of methanogenic fermentation with additional processes to achieve improved carbon efficiency.

The application discloses a non-conventional methanogenic archaea and belongs to the technical field of microorganisms. The methanogenic archaea (Methanoproducendum sp.) DRTY01 has a preservation number of CCTCC NO: M 2025822 and is preserved in the China Center for Type Culture Collection, is the first archaea with a methanogenic function isolated at present, and is a new genus representative in the Archaeoglobaceae family. The methanogenic archaea DRTY01 is also the only strain capable of efficiently utilizing methanol to produce methane under the condition of 85 DEG C ultra-high temperature, thereby providing an efficient, environment-friendly and feasible technical scheme for energy supply under specific scenes such as an ultra-high temperature fermentation tank.

The present invention provides an alkali-resistant and high-temperature-resistant methanogenic archaeon, a culture medium and a screening method. The alkali-resistant and high-temperature-resistant methanogenic archaeon is named MZD129, which has been deposited in the General Microbiological Center of China Microbiological Culture Collection Committee on May 14, 2024. The deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with a deposit number of CGMCC No. 41192 and a classification name of Methanoculleus bourgensis The bacteria has the characteristics of high methane production, alkali resistance, high temperature resistance and can grow in a simple synthetic culture medium. The invention effectively overcomes the shortcomings of traditional methanogenic archaea that are difficult to cultivate and have harsh environmental conditions, while realizing the resource utilization of waste and solving the problem of the difficulty in cultivating methanogenic archaea.

A method of converting CO2 gas produced during industrial processes comprising contacting methanogenic archaea with the CO2 gas under suitable conditions to produce methane.

The invention relates to the technical field of animal husbandry, in particular to application of an additive prepared from gracilaria lemaneiformis and used for reducing methane emission of fattening beef cattle. The gracilaria lemaneiformis is naturally sun-dried or dried gracilaria lemaneiformis. The dried gracilaria lemaneiformis powder is added into the mixed basic ration, the activity of archaea related to methane production can be inhibited, meanwhile, the abundance of bacteria which compete with a substrate of the methanogenic archaea is increased, the fermentation process is optimized by adjusting the relative proportion of volatile fatty acids such as acetic acid, propionic acid and butyric acid in rumen, and the yield of the methanogenic archaea is increased. And the method has important value and significance in the green emission reduction aspect of the breeding industry, and has great industrial application potential.

A method for controlling the activity of sulfate reducing bacteria or methanogenic archaea (or both) in environments containing such organisms comprising treating the environment with free nitrous acid (HNO2) or with a solution containing nitrite (NO2−) having a pH of less than 7 or by adding nitrite to the environment and having a pH of less than 7 in the environment. The method can also disrupt biofilms.

The invention relates to the technical field of anaerobic digestion of crop straw raw materials, and particularly discloses a method for strengthening anaerobic methanation of crop straw based on biological micro-aerobic pretreatment combined with a microbial electrolysis cell. According to the method, crop straw is placed in a closed system added with calcium peroxide (CaO2) and inoculated sludge for biological micro-aerobic pretreatment. A micro-aerobic environment created by CaO2 is utilized to stimulate facultative hydrolytic bacteria to enrich and secrete a large amount of hydrolase, and the lignocellulose structure of the straw is efficiently cracked. After pretreatment, the system is transferred into a microbial electrolysis cell reactor, inhibition of residual oxidizing substances in pretreatment on methanogenic archaea is effectively eliminated by utilizing a cathode reduction effect, and meanwhile, electron transfer and methanogenic metabolism paths of the system are enhanced. Through a coupling strategy of'biological micro-aerobic pretreatment + microbial electrolysis cell ', synergistic improvement of hydrolysis and methanation processes is realized, and an innovative and feasible solution is provided for efficient anaerobic digestion of crop straws to produce methane.

The application provides a method for repairing a chlorinated hydrocarbon contaminated site in situ and application thereof, the method injects polymer particles into the chlorinated hydrocarbon contaminated site for fermentation for a certain time, the polymer particles provide a carbon source and an electron donor required for in-situ microbial growth, meanwhile, the polymer particles inhibit the production of methane by methanogenic archaea in the in-situ microorganisms, help the chlorinated hydrocarbon degrading bacteria in the in-situ microorganisms to compete for the electron donor, improve the utilization of the electron donor by the chlorinated hydrocarbon degrading bacteria, and promote the degradation of the chlorinated hydrocarbon; the polymer particles are biodegradable high molecular polymer particles. By adjusting the particle size of the biodegradable high molecular polymer particles to 10 2 microns, the 10 2 micron biodegradable high molecular polymer particles can selectively inhibit the functional characteristics of the metabolism of methanogenic archaea, help the chlorinated hydrocarbon degrading bacteria to compete for the electron donor, thereby improving the utilization efficiency of the electron donor by the chlorinated hydrocarbon degrading bacteria, and improving the repair effect of the chlorinated hydrocarbon.