Preparation method of methane dry fermentation compound bacterial preparation

A compound bacterial agent and dry fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of narrow application range and no mention of dry fermentation, and achieve low cost, rich enzyme production, The effect of reducing volume

Inactive Publication Date: 2014-07-30
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its microbial composition is mainly designed for the degradation of cellulose, and it is used for conventional biogas fermentation with rice straw as the main raw material. The use range of this microbial agent is narrow, and it is not mentioned for dry fermentation

Method used

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  • Preparation method of methane dry fermentation compound bacterial preparation
  • Preparation method of methane dry fermentation compound bacterial preparation
  • Preparation method of methane dry fermentation compound bacterial preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The compound strain of the present invention is made up of 11 strains of bacteria, according to the difference in function in the fermentation process, these 11 strains of bacteria can be divided into following 6 classes:

[0044] Cellulolytic bacteria: Bacteroides cellulosilyticus , Clostridium sufflavum ;

[0045] Proteolytic bacteria: Clostridium pasteurianum ;

[0046] Lipolytic bacteria: Syntrophomonas sapovoran s;

[0047] Sulfate-reducing bacteria: Desulfovibrio desulfuricans , Desulfobulbus rhabdoformis ;

[0048] Hydrogen-producing acetogenic bacteria: Syntrophomonas Wolfei ;

[0049] Methanogenic archaea: Methanospirillum hungatei , Methanocorpusculum parvum , Methanosarcina barkeri , Methanosaeta concilii .

[0050] The above-mentioned 11 strains belonging to cellulolytic bacteria, proteolytic bacteria, lipolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria and methanogenic archaea were directly mixed ...

Embodiment 2

[0054] The composition ratio of the 11 strains in the inoculum is as follows: Bacteroides cellulosilyticus Accounted for 22%, Clostridium sufflavum Accounted for 17%, Clostridium pasteurianum accounted for 10%, Desulfobulbus rhabdoformis accounted for 11%, Syntrophomonas sapovorans accounted for 11%, Syntrophomonas Wolfei accounted for 10%, Desulfovibrio desulfuricans accounted for 13%, Methanospirillum hungatei Accounting for 0.5%, Methanocorpusculum parvum accounted for 1.0%, Methanosarcina barkeri Accounting for 1.5%, Methanosaeta concilii Accounted for 3%.

[0055] A mixture of pig manure and corn stalks was used as the fermentation substrate. Corn stalks were crushed into powder, mixed with pig manure at a mass ratio of 1:1, water was added to adjust the TS content to 30%, and stacked at natural temperature for 7 days. After stacking and retting, adjust the pH value to 7.0 with lime powder, then add the above inoculum according to raw material: inocu...

Embodiment 3

[0058] The inoculum consisted of the following 11 strains, Bacteroides cellulosilyticus accounted for 20%, Clostridium sufflavum accounted for 15%, Clostridium pasteurianum accounted for 12%, Desulfobulbus rhabdoformis accounted for 11%, Syntrophomonas sapovorans accounted for 13%, Syntrophomonas Wolfei accounted for 10%, Desulfovibrio desulfuricans accounted for 13%, Methanospirillum hungatei accounted for 1.0%, Methanocorpusculum parvum Accounting for 1.5%, Methanosarcina barkeri 2% Methanosaeta concilii Accounted for 1.5%.

[0059] A mixture of pig manure and rice straw is used as the fermentation substrate. Rice straw is crushed into powder, mixed with pig manure at a mass ratio of 1:1, water is added to adjust the TS content to 25%, and stacked at natural temperature for 7 days. After stacking, adjust the pH value to 6.5 with lime powder, and then add the above-mentioned inoculum according to the fermentation substrate: inoculum = 10:3 (mass ratio). ...

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Abstract

The invention discloses a preparation method of a biogas dry fermentation composite bacterial agent, which uses the composite bacteria composed of cellulolytic bacteria, proteolytic bacteria, lipolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria and methanogenic archaea as Inoculum, mix the inoculum with the fermentation substrate, adjust the moisture content of the mixture to 70-80%, and the pH value to 7.0, carry out anaerobic fermentation at 35°C±2°C, and obtain biogas after 60-90 days of fermentation Dry fermentation compound bacterial agent. The invention can prepare a composite bacterial agent with relatively complete biogas fermentation function microorganisms. The total number of microbial cells in the microbial agent is at least 1.0×1010 / g, and the total number of methanogenic archaeal cells is at least 1.0×108 / g. Using it as an inoculum for biogas dry fermentation can shorten the start-up time, and the fermentation process is stable and easy to control.

Description

technical field [0001] The invention relates to a preparation method of a biogas dry fermentation composite bacterial agent, belonging to the technical fields of organic waste utilization and renewable energy production. Background technique [0002] In recent years, society's demand for energy has been increasing, fossil energy resources such as petroleum and coal have been decreasing, and prices have continued to rise, which has brought adverse effects on people's production and life. workers' attention. Biogas is clean and renewable energy, which is inexhaustible. Organic matter such as straw, poultry manure, and urban organic waste can be converted into biogas through anaerobic digestion, providing clean energy for humans. [0003] Microorganisms involved in biogas fermentation include fermentative bacteria, hydrogen-producing acetogenic bacteria, and methanogenic archaea. First, fermentative bacteria degrade cellulose substances into organic acids such as acetic acid...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01C12R1/145
Inventor尹小波李强徐彦胜马诗淳张云飞代丽蓉承磊
OwnerBIOGAS SCI RES INST MIN OF AGRI