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A kind of method for producing propionic acid by fermentation with mixed bacteria system

A system, propionic acid technology, applied in the field of microorganisms, can solve the problems of high input-output ratio, can not meet the needs of industrialization, limited strain sources, etc., and achieve the effect of low input and output, which is conducive to the implementation of industrialization

Active Publication Date: 2020-04-17
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this technical solution has reached a relatively high level in terms of propionic acid concentration and purity, the propionic acid bacterial strain it adopts is a preserved special bacterial strain, and it is necessary to add yeast separately to avoid the introduction of other miscellaneous bacteria. Influenced by the mixed bacteria system, not only the source of strains is limited, but also the cost is relatively high; in addition, the product is only propionic acid, which has a high input-output ratio and low industrial value
[0006] Large-scale industrialization requires raw materials with wider sources and higher cost performance, but the existing technology obviously cannot meet the needs of industrialization, and can only be theoretical research at the laboratory stage

Method used

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  • A kind of method for producing propionic acid by fermentation with mixed bacteria system
  • A kind of method for producing propionic acid by fermentation with mixed bacteria system

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Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1: fermentation method of the present invention (first round of fermentation)

[0043] The medium composition that this embodiment adopts is:

[0044] NH 4 Cl, 530mg / L; KH 2 PO 4 2H 2 O, 200mg / L; Na 2 SO 4 , 40mg / L; KCl, 50mg / L; CaCl 2 , 10mg / L; MgCl 2 ·6H 2 O, 70 mg / L; MnCl 2 4H 2 O, 0.8mg / L; CoCl 2 2H 2 O, 1.2mg / L; FeSO 4 ·7H 2 O, 3.2mg / L; AlCl 3 , 0.5mg / L; NaMO 4 2H 2 O, 1 mg / L; H 3 BO 3 , 0.2mg / L; NiCl 2 ·6H 2 O, 0.5mg / L; CuCl 2 2H 2 O, 1.1mg / L; ZnSO 4 2H 2 O, 3.2mg / L; EDTA (Na + type), 3.0mg / L.

[0045] The present embodiment anaerobic sludge flora composition:

[0046] Methanosaeta 8%, Methanobacterium 0.4%, Dethiosulfovibrionaceae 9.1%, Anaerolinaceae 7.1%, Streptococcaceae 6.9%, Syntrophobacteraceae 4.2%, Cloacamonaceae 3.7%, Syntrophorhabdaceae 3.5%, SB-1 3.4%, Geobacteraceae 3.1%, Clostridiaceae 2.5%, Porphyromonadaceae 1.9%, Pseudomonadaceae 1.7%, Syntrophomonadaceae 1.6%, [Tissierell...

Embodiment 2

[0056] Embodiment 2: fermentation method (second round of fermentation) of the present invention

[0057] The solid matter containing the flora of Example 1 and the anaerobic fermentation medium (same as Example 1) are used to form a fermentation system, the pH of the fermentation system is adjusted to 7.0-7.3, the temperature is 35-37°C, and the fermentation system is fed every day Add 5 g of glycerol to the fermentation system to make the concentration of organic matter between 0.1-5 g / L, and gradually increase the concentration of ammonium ions in the fermentation system at a rate of 0.3 g / (L·d) until the concentration of ammonium ions in the fermentation system is 1g / L, the process time is generally 7-20 days.

[0058] After the ammonium ion concentration in the fermentation system is stabilized at 1g / L, keep the ion concentration constant, and continue to add organic glycerol to the reaction system in the sequential batch mode to maintain the organic concentration between...

Embodiment 3

[0062] Embodiment 3: fermentation method of the present invention (first round+second round of fermentation)

[0063] According to the method of embodiment 1 and embodiment 2, with being different from the anaerobic sludge in the anaerobic fermentation tank of another sewage treatment plant of embodiment 1 as starting material, flora composition is as follows:

[0064] Methanosaeta 10%, Methanobacterium 2.5%, Anaerolinaceae 15.5%, Streptococcaceae 12.8%, Cloacamonaceae 6.5%, Thermotogaceae 6.2%, Dethiosulfovibrionaceae 4.2%, Ruminococcaceae 3.6%, Enterobacteriaceae 2.7%, Clostridiaceae 2.5%, Peptococcaceae 2.3%, Syntrophomonadaceae 1.9%, Porphyromonadaceae 1.7%, Geobacteraceae 1.7%, Gracilibacteraceae 1.6%, Synergistaceae 0.9%, Pseudomonadaceae 0.6%, Sphaerochaetaceae 0.5%, other 22.3% (content less than 0.5% and unclassified flora not listed).

[0065] After the first round of fermentation, it was tested that the purity of prop...

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Abstract

The invention relates to the technical field of microbes, and discloses a method for producing propionic acid by mixed bacteria system fermentation. The method comprises the following steps: carrying out sequencing batch anaerobic fermentation on a fermentation system composed of Methanogenic archaea / propionic-acid-producing bacteria-containing anaerobic sludge and an anaerobic fermentation culture medium, wherein in the fermentation process, the ammonium ion concentration in the fermentation system is enhanced to 1-2 g / L and kept constant, and methane is collected; and after the fermentation finishes, collecting the fermentation liquid, extracting the propionic acid, and adding the residual flora-containing solid into the anaerobic fermentation culture medium for the next fermentation round. The ammonium ion concentration is regulated to control the flora distribution of the Methanogenic archaea / propionic-acid-producing bacteria-containing anaerobic sludge, so that the fermentation environment is beneficial to the proliferation of the propionic-acid-producing bacteria; and the method can obtain a large amount of methane in the process of producing high-concentration high-purity propionic acid, has the advantages of unrestricted material sources and lower input-output ratio, and is beneficial to industrialized implementation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for producing propionic acid by fermentation in a mixed bacteria system. Background technique [0002] Propionic acid and propionate are very important chemicals that can be widely used as raw materials in food, feed, and pharmaceutical industries. With the continuous development of these industries, the demand for propionic acid is also increasing, but the amount of propionic acid produced in China is far from enough, and it needs to rely on a large amount of imports every year. Therefore, it seems necessary to develop propionic acid production and develop new propionic acid production processes. very urgent. [0003] Propionic acid is mainly derived from chemical synthesis and microbial fermentation. In industry, it is mainly synthesized through aldehyde liquefaction obtained from fossil raw materials such as carbonylation of ethylene and carbon monoxide, propa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/52C12P5/02C12R1/01C12R1/145
CPCC12P5/023C12P7/52C12P39/00Y02E50/30
Inventor 曾建雄王婷陈赟
Owner UNIV OF SCI & TECH OF CHINA
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