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Methane dry fermentation compound bacteria

A technology of compound bacteria and dry fermentation, applied in the fields of fermentation, bacteria, biochemical equipment and methods, etc., can solve the problems of microbial compound bacteria that are not involved in dry biogas fermentation, and achieve remarkable effect, high microbial concentration, and enhanced stability. Effect

Inactive Publication Date: 2014-06-18
BIOGAS SCI RES INST MIN OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In the published literature, domestic researchers used anaerobic activated sludge, municipal sludge, and biogas residue from rural biogas tanks as inoculum in the research of biogas dry fermentation with organic waste, straw, and livestock manure as raw materials. However, none of them involved artificially constructed biogas dry fermentation microbial complexes

Method used

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  • Methane dry fermentation compound bacteria
  • Methane dry fermentation compound bacteria
  • Methane dry fermentation compound bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The mixed raw material of pig manure and rice straw powder with a mass ratio of 5:1 was used as the fermentation substrate. The cell number contents of 11 bacterial strains in the composite bacteria are respectively: Bacteroides cellulosilyticus accounted for 15%, Clostridium sufflavum accounted for 11%, Clostridium pasteurianum accounted for 15%, Desulfobulbus rhabdoformis Accounted for 16%, Syntrophomonas sapovorans 9% Syntrophomonas Wolfei accounted for 12%, Desulfovibrio desulfuricans Accounted for 16%, Methanospirillum hungatei Accounting for 0.5%, Methanocorpusculum parvum 1% Methanosarcina barkeri Accounted for 2.5%, Methanosaeta concilii Accounted for 3%. Dry fermentation after mixing the above-mentioned composite bacteria and fermentation substrate. After 60-90 days of fermentation, the indicators are shown in the table below:

[0049]

Embodiment 2

[0051] The mixed raw material of pig manure and rice straw powder with a mass ratio of 4:1 was used as the fermentation substrate. The cell number contents of the 11 strains in the composite bacteria are: Bacteroides cellulosilyticus Accounted for 17%, Clostridium sufflavum accounted for 12%, Clostridium pasteurianum accounted for 14%, Desulfobulbus rhabdoformis accounted for 15%, Syntrophomonas sapovorans 9% Syntrophomonas Wolfei accounted for 11%, Desulfovibrio desulfuricans accounted for 15%, Methanospirillum hungatei Accounting for 0.5%, Methanocorpusculum parvum Accounting for 1.5%, Methanosarcina barkeri Accounted for 2.5%, Methanosaeta concilii Accounted for 2.5%. Dry fermentation after mixing the above-mentioned composite bacteria and fermentation substrate. After 60-90 days of fermentation, the indicators are shown in the table below:

[0052]

Embodiment 3

[0054] The mixed raw material of pig manure and rice straw powder with a mass ratio of 3:1 was used as the fermentation substrate. The cell number contents of 11 bacterial strains in the composite bacteria are respectively: Bacteroides cellulosilyticus Accounted for 18%, Clostridium sufflavum accounted for 14%, Clostridium pasteurianum accounted for 14%, Desulfobulbus rhabdoformis accounted for 14%, Syntrophomonas sapovorans 9% Syntrophomonas Wolfei accounted for 10.5%, Desulfovibrio desulfuricans accounted for 15%, Methanospirillum hungatei Accounting for 0.5%, Methanocorpusculum parvum 1% Methanosarcina barkeri Accounting for 1.5%, Methanosaeta concilii Accounted for 2.5%. Dry fermentation after mixing the above-mentioned composite bacteria and fermentation substrate. After 60-90 days of fermentation, the indicators are shown in the table below:

[0055]

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Abstract

The invention discloses methane dry fermentation compound bacteria, which comprise 25% to 45% of cellulose decomposing bacteria, 9% to 15% of protein decomposing bacteria, 8% to 15% of fat decomposing bacteria, 8% to 12% of hydrogen-producing acetogenic bacteria, 18% to 31% of sulfate reducing bacteria and 2.5% to 9.5% of methanogenic archaea according to bacterium number percentages. The compound bacteria of microorganisms with a complete methane fermentation function are capable of processing methane dry fermentation raw materials with TS content as 20% to 30% and stable in methane production effect and apt to control methane production.

Description

technical field [0001] The invention relates to the utilization of organic waste and the development of renewable energy, in particular to a biogas dry fermentation composite bacterium, which belongs to the technical field of biological fermentation. Background technique [0002] In recent years, society's demand for energy has been increasing, fossil energy resources such as oil and coal have been decreasing, and prices have continued to rise, which has brought adverse effects on social and economic development. The development and utilization of renewable energy has attracted more and more attention from governments and scientific researchers in various countries. Biogas is a clean renewable energy that is inexhaustible. Organic matter such as straw, poultry manure, and domestic waste can be converted into biogas through anaerobic digestion, providing clean energy for humans. [0003] Microorganisms involved in biogas fermentation include fermentative bacteria, hydrogen-p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P39/00C12P5/02C02F11/04C12R1/145C12R1/01
CPCY02E50/343Y02E50/30
Inventor 尹小波徐彦胜李强马诗淳张云飞代丽蓉承磊
Owner BIOGAS SCI RES INST MIN OF AGRI
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