Transportation culturing device

A culture device and tube body technology, which is applied in biochemical cleaning devices, enzymology/microbiology devices, biochemical instruments, etc., can solve the problems of cross-contamination, low positive detection rate, inconvenient operation, etc., and achieve the enrichment efficiency. The effect of improving, preventing cross-contamination, and satisfying convenience

Active Publication Date: 2019-04-16
泰普生物科学(中国)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the companies that produce group B Streptococcus nucleic acid detection kits on the market include Boercheng, Shanghai Zhizhi and Antu, etc. None of the above companies are equipped with special transport media; and the B Streptococcus group nucleic acid detection kit (fluorescent PCR method) has been promoted in the market for many years, and has a place in the obstetrics and gynecology market, but when its first-generation sample preservation solution (model: GBS) is used on its consumables, due to the need Relying on the professional sampling of professionals, and the swabs need to be broken during the sampling process, which is inconvenient in operation; and if the samples are not properly stored or transported during the transportation process, bacteria cannot be enriched, resulting in positive results The detection rate is low; in addition, during the detection process, it is necessary to open the cap of the tube and use a pipette to draw liquid for detection, so there is a high probability of cross-contamination of samples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Please refer to Figure 1-7 , Embodiment 1 of the present invention is:

[0039] The transport culture device of this embodiment includes a first tube body 1, a second tube body 2, a first sealing cover body 3 and a second sealing cover body 4, and the first tube body 1 and the second tube body 2 are respectively A tubular structure with openings at both ends, the inner diameter of the first tubular body 1 is larger than the outer diameter of the second tubular body 2, and one end of the second tubular body 2 is sheathed on the second tubular body via one end of the first tubular body 1. A tube body 1 can be pushed and pulled along the length direction of the first tube body 1, the first sealing cover 3 is set on the other end of the first tube body 1, and the second sealing cover 4 is set on the other end of the first tube body 1 The other end of the second tube body 2; the length of the overall tube body structure formed by the first tube body 1 and the second tube b...

Embodiment 2

[0053] The above-mentioned sample preservation solution (model: GBS) stored and transported by the transport culture device of Example 1 was subjected to a bacteria enrichment experiment, so as to test and verify the storage performance of the transport culture device of the present invention.

[0054] For details, see the following instructions:

[0055] 1. Purpose of the experiment

[0056] 1.1 Determination of the bacterial enrichment effect of the sample preservation solution (model: GBS) at 37°C and room temperature

[0057] 2. Preparation before the experiment

[0058] 2.1GBS Quantitative Kit

[0059] 2.2 Sample preservation solution (model: GBS): The sample preservation solution in this experiment is the same batch number (batch number: 65170J, production date: 2017.10.23).

[0060] Table 1

[0061] mark number

Program concentration

Culture temperature

Preservation solution 1

102 copies / ml

37℃

Preservation solution 2

102 cop...

Embodiment 3

[0080] The growth curve and sample detection rate of the above-mentioned sample preservation solution (model: GBS) stored and transported by the transport culture device of Example 1 were verified, so as to test and verify the storage performance of the transport culture device of the present invention. For details, see the following instructions:

[0081] 1. Purpose of the experiment

[0082] 1.1 Measure the growth curve of the sample preservation solution (model: GBS);

[0083] 1.2 Take the company as a small unit, collect samples, operate according to the instructions of the sample preservation solution, and measure the detection rate.

[0084] 2. Preparation before the experiment

[0085] 2.1 GBS reaction solution (321707, 2 boxes from the warehouse)

[0086] 2.2 Sample preservation solution (model: GBS) (prepared in workshop, 30ml, batch number 631709)

[0087] 2.3 GBS original bacteria activation: preparation of Lim medium → add 3ml of Lim medium to a 5ml centrifuge ...

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Abstract

The invention relates to a transportation culturing device. The device comprises a first pipe body, a second pipe body, a first sealing cover body and a second sealing cover body, the first and secondpipe bodies are of tubular structures with openings formed in two ends respectively, the inner diameter of the first pipe body is greater than that of the second pipe body, one end of the first pipebody sleeves one end of the second pipe body, the second pipe body can be pushed and pulled in the length direction of the first pipe body, the first sealing cover body covers the other end of the first pipe body, and the second sealing cover body covers the other end of the second pipe body; the length of an integrated pipe body structure formed by the first and second pipe bodies is matched withthe length of a swab which is used for taking materials. The transportation culturing device can meet sealing and sterile requirements, so that it is ensured the culture medium is not leaked or contaminated by bacteria in the transporting and preserving process, the using convenience is met, and a non-professional worker can achieve professional material taking.

Description

technical field [0001] The invention relates to a sample collection and transport device, in particular to a transport and culture device. Background technique [0002] The main route of transmission of Group B Streptococcus is vertical transmission, and it is related to childbirth. If a pregnant woman is infected with Group B Streptococcus, the newborn may be infected with Group B Streptococcus during inhalation of infected amniotic fluid or passing through the birth canal. Neonatal infection with Group B Streptococcus can lead to sepsis, pneumonia, and meningitis, with high morbidity and mortality, and may also leave behind long-term pathological conditions such as deafness, visual impairment, developmental disabilities, and cerebral palsy. [0003] At present, the companies that produce group B Streptococcus nucleic acid detection kits on the market include Boercheng, Shanghai Zhizhi and Antu, etc. None of the above companies are equipped with special transport media; and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/26C12M1/00
CPCC12M23/08C12M23/38C12M33/02
Inventor 蔡晓沂林燕英姚雪
Owner 泰普生物科学(中国)有限公司
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