Intelligent pH-catalytic-response micro-nano robot and assembling method and application thereof

An assembly method and robot technology, which can be applied in drug combinations, pharmaceutical formulations, organic active ingredients, etc., can solve problems that have not been reported in research, and achieve the effects of good targeting, fast speed, and high drug load

Active Publication Date: 2019-04-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, micro-nanorobots constructed from MOFs have attracted widespread attention, however, the study of designing a biological trigger to control micro-nanorobots has not been reported yet.

Method used

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  • Intelligent pH-catalytic-response micro-nano robot and assembling method and application thereof
  • Intelligent pH-catalytic-response micro-nano robot and assembling method and application thereof
  • Intelligent pH-catalytic-response micro-nano robot and assembling method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This embodiment provides a method for assembling an intelligent pH catalysis-responsive micro-nano robot, which includes the following steps:

[0027] Step 1. Succinylated β-lactoglobulin is modified to obtain succinylated β-lactoglobulin.

[0028] The specific process is as follows: first dissolve 100 mg of β-lactoglobulin in 10 mL of phosphate buffer (50 mM, pH=7.4). Then, with stirring at room temperature, 25 mg of succinic anhydride was slowly introduced. Sodium hydroxide solution (1M) was added to maintain the pH value at 7.5-8.5, and the solution was stirred for 1.5 h. Then the solution was transferred to a dialysis bag (5000-7500 NMWCO), and placed in a glass beaker filled with 1 L of ultrapure water (4°C), and dialyzed for 24 hours, during which the dialysate outside the bag was replaced every 3 hours. Finally, the resulting solution was transferred to a round-bottom flask to freeze-dry to obtain succinylated β-lactoglobulin, which was called succinylated β-la...

Embodiment 2

[0036] This example is an evaluation of the motion performance of the Cat-β@ZIF micro-nano robot prepared in Example 1.

[0037] In order to objectively evaluate the movement performance of the Cat-β@ZIF micro-nano robot prepared in Example 1, this example tested the autonomous movement of the Cat-β@ZIF micro-nano robot under different hydrogen peroxide concentrations and pH conditions. The real-time movement of individual microrobots in X and Y coordinates is recorded by utilizing Nanoparticle Tracking Analyzer (NTA) and provides individual particle movement analysis. And this data is further used to plot the mean square displacement (MSD) curve of the particle, and the diffusion coefficient of the robot particle is calculated with the obtained MSD value.

[0038] image 3 It is the motion performance analysis diagram of the Cat-β@ZIF micro-nano robot in the first embodiment of the present invention.

[0039] Such as image 3 as shown, image 3 (a) Diffusion coefficient m...

Embodiment 3

[0044] In this example, the Cat-β@ZIF micro-nano robot prepared in Example 1 is used as a carrier to load the main drug to prepare a tumor treatment drug. In this embodiment, the main drug is doxorubicin (DOX).

[0045] The preparation process of the tumor treatment drug is as follows:

[0046] Add 1mg DOX to 1mL Cat-β@ZIF micro-nano robot particle aqueous solution (1.724mM DOX), after stirring slowly for 1h, centrifuge to remove the supernatant, and then wash to obtain the tumor treatment drug, which is loaded with DOX's Cat-β@ZIF micro-nano robot.

[0047] The DOX concentration in the supernatant was calculated from the concentration-absorbance standard curve, and the DOX loading rate of the Cat-β@ZIF micro-nano robot was as high as 83%.

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Abstract

The invention provides an intelligent pH-catalytic-response micro-nano robot. The intelligent pH-catalytic-response micro-nano robot is characterized by comprising a porous skeleton internally and totally loaded with succinylation beta-lactoglobulin and catalase, wherein the succinylation beta-lactoglobulin is beta-lactoglobulin modified by succinylation, and the porous skeleton is a ZIF-L metal organic frame. The invention provides an assembling method for the intelligent pH-catalytic-response micro-nano robot, and the assembling method includes the following steps that 1, the beta-lactoglobulin is subjected to succinylation modification to obtain the succinylation beta-lactoglobulin; 2, the succinylation beta-lactoglobulin, the catalase and a 2-methylimidazole water solution are mixed, azinc ion solution is added, the mixture continues to stir for preset time, centrifugal separation is carried out, and the intelligent pH-catalytic-response micro-nano robot is obtained. The inventionprovides application of the intelligent pH-catalytic-response micro-nano robot as a carrier to preparing tumor treatment medicine.

Description

technical field [0001] The invention belongs to the field of artificial micro-nano robots, and in particular relates to an intelligent pH catalytic response type micro-nano robot, its assembly method and application. Background technique [0002] Micro-nano robots are micro-nano devices that convert other forms of energy into mechanical motion. Due to its small size, autonomous movement and strong loading capacity, it not only has great application potential in the fields of biosensing, microsurgery, medical imaging and environmental governance, but is also expected to be used for targeted delivery of drugs to achieve treatment of diseased cells. precision treatment. In recent years, a lot of research has been done on chemically self-propelled micro-nanorobots. Inspired by biomolecules and self-propelled microorganisms, artificial micro-nano robots can use catalytic reactions to provide energy for autonomous movement. At present, the motion control of chemical self-propel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/24A61K31/704A61P35/00
CPCA61K31/704A61K47/24A61K47/42A61P35/00
Inventor 孔彪曾洁张威赵东元
Owner FUDAN UNIV
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