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A method for producing low-viscosity Paenibacillus polymyxa fermentation broth

The technology of polymyxa spore and fermentation liquid is applied to the fermentation medium of Paenibacillus polymyxa to produce low-viscosity Paenibacillus polymyxa fermentation liquid, which can solve the problem of increasing production cycle and cost, increasing the difficulty of processing fermentation liquid, Pseudoplasticity and other problems, to achieve the effect of reducing production cycle and cost, simplifying post-processing separation and purification process, and improving fermentation level

Active Publication Date: 2021-11-26
WUHAN KERNEL BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because Paenibacillus polymyxa will secrete a large amount of viscous substances during the fermentation process, it has the characteristics of pseudoplasticity and high viscosity, and has good stability to heat and acid and alkali. These characteristics increase the difficulty of processing the fermentation broth. It is necessary to adjust the pH, heat, add filter aids and other methods to the fermentation broth to separate and purify the high-quality Paenibacillus polymyxa powder, which increases the production cycle and cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The strain used to produce Paenibacillus polymyxa fermentation broth comes from Wuhan Kono Biotechnology Co., Ltd., named Paenibacillus polymyxa (BaciIllus polymyxa) KN-03, and the preservation number is CCTCC NO: M 2012077. One has been disclosed in the patent document of CN 104611254A, but the embodiments of the present invention are not limited to KN-03.

[0013] 1. Strain activation and seed cultivation

[0014] First, the KN-03 strain is subjected to strain activation and seed cultivation according to a conventional method to obtain a seed liquid, and the specific steps are as follows:

[0015] 1) Strain activation: Pick a ring of Paenibacillus polymyxa KN-03 and spread it on the slant medium, and place the inoculated slant in a water-proof constant temperature incubator at 30°C for 72 hours.

[0016] Slant medium: 20g glucose, 200g peeled potatoes (boil in tap water for 30min), 15g agar powder, 1000ml tap water, natural pH, mix the above materials and sterilize a...

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Abstract

The invention discloses a method for producing a low-viscosity Paenibacillus polymyxa fermentation liquid, which belongs to the field of microbial fermentation. The method is to add 0.15 to 1% of L-Tianmen by weight to the fermentation medium of Paenibacillus polymyxa Partic acid achieved. The invention not only increases the number of viable spores in the fermentation liquid, improves the existing fermentation level, but also reduces the viscosity of the fermentation liquid, simplifies the post-treatment separation and purification process, thereby reducing the production cycle and cost of the Paenibacillus polymyxa powder , has significant economic benefits.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to a method for producing a low-viscosity Paenibacillus polymyxa fermentation liquid, and also relates to a fermentation medium of Paenibacillus polymyxa. Background technique [0002] Paenibacillus polymyxa can produce various antibacterial substances such as peptides, proteins, nucleosides, pyrazines and phenols, and can prevent and control various plant diseases caused by pathogenic bacteria, pathogenic fungi and root-knot nematodes. In addition, Paenibacillus polymyxa has the ability to move and colonize from the soil to the roots of plants, thereby competing with pathogenic bacteria for space and nutrients. Plants provide nutrients. Therefore, Paenibacillus polymyxa is widely used in agricultural production, and the demand for Paenibacillus polymyxa is also increasing year by year. [0003] Currently commercially available Paenibacillus polymyxa is mainly a wettable powder...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/12
CPCC12N1/20
Inventor 潘渝周莉刘守德万俊李青刘华梅程治国
Owner WUHAN KERNEL BIO-TECH CO LTD