Method for measuring cefalexin concentration in plasma through hygroplasm combination

A technology of cephalexin and liquid mass spectrometry, which is applied in the field of medicine, can solve the problems of cumbersome production process and high production cost of immunochromatographic detection test strips or test boxes, and achieve simple pretreatment method, simple operation and fast method Effect

Inactive Publication Date: 2019-04-26
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the detection speed of immunoassay technology is fast, the production process of the immunochromatographic detection test strips or test kits applied to this technology is cumbersome and the production cost is high

Method used

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  • Method for measuring cefalexin concentration in plasma through hygroplasm combination
  • Method for measuring cefalexin concentration in plasma through hygroplasm combination
  • Method for measuring cefalexin concentration in plasma through hygroplasm combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Example: Human K 2 Determination of Cefalexin Concentration in EDTA Plasma

[0034] 1. Experimental materials and analytical equipment

[0035] Cephalexin (analyte): China National Institutes for Food and Drug Control or equivalent or higher grade standard

[0036] Cefalexin-d5 (internal standard): Toronto Research Chemicals or equivalent or higher grade standard

[0037] The reagents used are shown in Table 1 below:

[0038] Table 1 Reagent Details

[0039] Reagent name

level

manufacturer

Acetonitrile

HPLC

J. T. Baker

Ammonium acetate

HPLC

J. T. Baker

formic acid

ACS

Adamas

[0040] Note: Reagents of the same grade or higher can also be used

[0041] The analytical equipment used is shown in Table 2 below:

[0042] Table 2 Details of equipment used

[0043]

[0044]

[0045] The same LC / MS / MS system can also be used.

[0046] 2. Liquid conditions

[0047] 1. Liquid chromatography conditio...

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Abstract

The invention discloses a method for measuring cefalexin concentration in plasma through hygroplasm combination. A hygroplasm combination system is adopted for measurement. The method comprises the steps that firstly, a to-be-measured sample is taken, a certain quantity of mixed organic solvent is added for conducting extraction twice, and after pretreatment, through chromatographic column separation, a mass spectrometry detector is used for detection. The method is quick to use, accurate, high in sensitivity and easy and convenient to operate, and the basis is provided for plasma concentration measurement of cefalexin. The linear range of a plasma standard curve is within 0.1-50 mug / mL, the within-run precision and between-run precision RSD are smaller than + / -15%, and the method is suitable for measurement of cefalexin concentration in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining the concentration of cephalexin in blood plasma by liquid mass spectrometry. Background technique [0002] Bacterial infection is an acute systemic infection caused by pathogenic bacteria or opportunistic pathogenic bacteria invading the blood circulation to grow and reproduce, producing toxins and other metabolites. Clinically, it is characterized by chills, high fever, rash, arthralgia, and hepatosplenomegaly. Some may have septic shock and migratory lesions. [0003] Cefalexin is a semi-synthetic oral cephalosporin antibiotic listed by Eli Lilly Company in the U.S. in 1970. It inhibits the synthesis of cell walls, causing the cell contents to expand to burst and dissolve, thereby achieving a bactericidal effect. This product is a broad-spectrum antibiotic, which has antibacterial effect on Gram-positive bacteria and Gram-negative bacteri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/34
CPCG01N30/34G01N30/88G01N2030/045G01N2030/342G01N2030/8822
Inventor 黄靖智
Owner 徐州立兴佳正医药科技有限公司
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