Application of Wuwei Mountain Upi Tea Extract

An extract and skin tea technology, which is applied in the application field of Wuwei Mountain Wupi tea extract, can solve the problems of short-term effect, high cost, edema or blood stasis, etc.

Active Publication Date: 2022-01-28
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the aforementioned methods, the molecular structure of ingredients such as collagen and hyaluronic acid is too large to be effectively absorbed by the human body through oral or smeared administration, so the actual supplementary effect is limited; although botulinum toxin can reduce skin cells However, the effect is short-lived, regular injections are required, and the cost is high. It may also cause side effects such as swelling or bruising at the injection site, drooping eyelids or eyebrows, headaches, allergies, asymmetrical left and right faces, or unnatural expressions.

Method used

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  • Application of Wuwei Mountain Upi Tea Extract
  • Application of Wuwei Mountain Upi Tea Extract
  • Application of Wuwei Mountain Upi Tea Extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: The effect of Wuwei Mountain Wupi tea extract on enhancing the antioxidant capacity of cells

[0053] It is known that when the content of reactive oxygen species (Reactive Oxygen Species, ROS) in skin cells is too high, cell tissue will be destroyed and DNA will be damaged, leading to skin aging. In order to avoid the toxicity of ROS to skin cells, organisms will secrete enzymes such as superoxide dismutase (SOD) to decompose excessive oxidative substances in the body. The following experiments were carried out to confirm the effectiveness of the Wuwei Mountain Wupi tea extract of the present invention in inhibiting the generation of ROS in skin cells and improving the SOD activity of skin cells.

[0054] ROS content detection

[0055] Human dermal fibroblasts (CCD-966SK; purchased from ATCC) were cultured in MEM medium (Minimum essential medium, purchased from Gibco, product number: 61100-061) for 24 hours, after which the cells were divided into four grou...

Embodiment 2

[0076] Example 2: The effect of Wuwei Mountain Wupi tea extract on enhancing the expression of SOD2, CAT and MSH2 genes

[0077] Human dermal fibroblasts were cultured in MEM medium for 24 hours. Then, the cells were divided into six groups and subjected to the following treatments:

[0078] Control group: the cells were cultured in MEM medium for 6 hours.

[0079] UVA group: the cells were cultured in MEM medium for 6 hours, and then the cells were irradiated with UVA (15 J / cm2) for 6 hours.

[0080]Extract (1)-6hr group and extract (1)-24hr group: the cells were placed in the MEM medium containing 1 mg per milligram [Preparation Example] Wuwei Mountain Wupi tea extract provided by culturing for 6 hours respectively hours and 24 hours, and then irradiate the cells with UVA (15 J / cm2) for 6 hours.

[0081] Extract (2)-6hr group and extract (2)-24hr group: the cells were placed in the MEM medium containing 2 mg per milliliter of the Wuwei Mountain Wupi tea extract provided in...

Embodiment 3

[0084] Example 3: The effect of Wuwei Mountain Wupi tea extract on enhancing the expression of FLG, HAS3, GBA, Tgm1 and Keratin14 genes

[0085] Human epidermal primary keratinocytes (HPEK-50; purchased from CELLnTEC, product number: PR3D-HPEK-50) were cultured in a serum-free medium (Keratinocyte-SFM; purchased from Thermo, product number: 17005042) for 24 hours. hr, then, the cells were divided into two groups and subjected to the following treatments:

[0086] Control group: cells were cultured in Keratinocyte-SFM medium for 6 hours.

[0087] Extract group: the cells were cultured in Keratinocyte-SFM medium containing 0.03125 mg per ml of the Wuweishan wupi tea extract provided in [Preparation Example] for 6 hours.

[0088] Thereafter, the above-mentioned groups of cells were collected respectively, RNA extraction was carried out with RNA extraction kit (RNA Extraction Kit, purchased from Geneaid Company), and then reverse transcriptase ( I II Reverse Transcriptase, purc...

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Abstract

The application of Wuwei Mountain Wupi tea extract, including the use of Wuwei Mountain Wupi tea extract in moisturizing, whitening, firming skin, reducing skin fine lines, anti-aging skin, improving dry skin, promoting the formation of hyaluronic acid in the skin, helping Applications for maintaining skin health or preparing pharmaceutical compositions. The pharmaceutical composition can be used for resisting light damage, repairing skin tissue, preventing skin diseases and / or treating skin diseases, or for at least one of the following: preventing cardiovascular diseases, treating cardiovascular diseases, preventing diabetes, treating diabetes, preventing Neurodegenerative diseases and treating neurodegenerative diseases.

Description

technical field [0001] The present invention relates to the application of Wuwei Mountain Wupi tea (Pyrenaria buisanensis) extract, including the use of Wuwei Mountain Wupi tea extract in moisturizing, whitening, firming skin, reducing skin fine lines, anti-aging skin, improving dry skin, promoting skin Formation of hyaluronic acid and / or help to maintain skin health; Diabetes, treating diabetes, preventing neurodegenerative diseases and / or treating neurodegenerative diseases. Background technique [0002] The skin is the body's first line of defense against external harmful factors, and has important functions such as water retention, warmth retention and sensation. Aging, as well as factors such as ultraviolet light, radiation, and environmental pollution, may reduce the water content of the skin, cause the loss of collagen and elastin, and lead to aging phenomena such as wrinkles, sagging skin, dullness, and roughness. Damage the DNA in skin cells, accelerate the death ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61Q19/00A61Q19/02A61Q19/08A61K36/82A61P17/16A61P17/02A61P17/00A61P9/00A61P3/10A61P25/28A61P9/10A23L33/105
CPCA23L33/105A23V2002/00A61K8/9789A61K36/82A61K2800/92A61P3/10A61P9/00A61P9/10A61P17/00A61P17/02A61P17/16A61P25/28A61Q19/00A61Q19/02A61Q19/08A23V2200/318A23V2250/214A61K9/0014A61K9/0019A61K2236/33A61Q19/004A61Q19/007A61P17/18A61K9/0053
Inventor 林咏翔林于婷
Owner TCI CO LTD
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