Automated device and method for detecting mycotoxins
An automated equipment and mycotoxin technology, applied in the field of analytical chemistry detection, can solve the problems of limiting food safety control timeliness, complex operation, high labor intensity, etc., and achieve the effects of reducing solvent replacement time, high detection sensitivity, and improving work efficiency
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[0038] Example 1: An automated equipment for detecting mycotoxins
[0039] (1) The composition of an automated equipment for detecting mycotoxins
[0040] Such as figure 1 As shown, an automated device for detecting mycotoxins includes: a control circuit system, a sample pre-processing system, a fluorescence detection system, and a data processing system.
[0041] The control circuit system includes a control circuit X and a power module Y. The sample pretreatment system includes low-pressure quaternary mixer A, pre-treatment solvent storage tanks A1-A4, first high-pressure constant flow pump B1, ternary switching valve C, first syringe pump D1, second syringe pump D2, and homogenizer E. Reactor F, two-position six-way switching valve G, trapping column I, waste bottle J, second high pressure constant flow pump B2 and elution solvent storage tank A5, sample bottle K, mobile phase storage tank A6, No. Three high pressure constant flow pump B3, autosampler H and chromatographic colum...
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[0049] Example 2 The automated equipment of the present invention is used for the detection of aflatoxin in the sample to be tested
[0050] Put the required solvents in the pretreatment solvent storage tanks A1-A4, the elution solvent storage tank A5 and the mobile phase storage tank A6, A1: methanol aqueous solution; A2: water; A5: methanol; A6: mobile phase.
[0051] The specific operations are as follows:
[0052] 1. Weigh 25g of the sample to be tested and place it in the homogenizer E, suck 125mL of the A1 solution in the homogenizer E through the first high-pressure constant flow pump B1 and the ternary switching valve C, and homogenize and stir for 2 minutes;
[0053] 2. Through the first syringe pump D1, draw 15mL of the sample extract in the homogenizer E and filter it in the reactor F, and then through the first high-pressure constant flow pump B1 and the three-way switching valve C to quantitatively suck the solution in 30mL A2 in the reaction In the reactor F, after react...
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