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Method for quickly and quantitatively detecting sulfaquinoxaline residue

A sulfaquinoxaline, quantitative detection technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of inconvenient large-scale screening and self-inspection of farms, inability to meet detection needs, and high requirements for instruments and equipment, and achieves easy popularization and popularization. Low cost, high sensitivity effect

Inactive Publication Date: 2019-05-14
ZHENJIANG YITE BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional detection methods, such as microbiological methods, gas chromatography, etc., can no longer meet the detection needs
At present, the legal detection standard method in various countries is HPLC (High Pressure Liquid Chromatography), which has high sensitivity, but the pretreatment process is complicated, and the requirements for instruments and equipment are high, so it is not convenient for farms to use for large-scale screening and self-inspection.

Method used

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  • Method for quickly and quantitatively detecting sulfaquinoxaline residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Detection of the upper soil polluted by sulfaquinoxaline from a pesticide factory in the suburbs

[0037] Milk sample treatment: Take the non-polluted milk sample and the milk sample polluted by sulfaquinoxaline from the pesticide factory in the suburbs, air-dry them, grind them and pass them through a 100-mesh sieve to get the blank sample and contaminated milk samples.

[0038] The measurement steps are:

[0039] Step 1, prepare milk sample supernatants with different final concentrations of sulfaquinoxaline: take 6 50mL conical flasks, add 0.50g blank milk sample to each, add sulfaquinoxaline standard solution respectively according to the concentration gradient of 1.0 to make it The final concentration of sulfaquinoxaline in the blank milk sample is 0, 1.0, 2.0, 3.0, 4.0, 5.0g / kg soil respectively, then add 10mL deionized water respectively, shake the shaker for 30min, place it for 30min, and put it in a centrifuge at 6000r / kg Centrifuge for 10 min, take ...

Embodiment 2

[0043] Example 2 Determination of sulfaquinoxaline content in the drainage ditch sulfaquinoxaline polluted water of a pesticide factory in a county in eastern Henan

[0044] Step 1, prepare standard aqueous solutions with different final concentrations of sulfaquinoxaline: take 6 test tubes, add 10ml of deionized water to each, and add the standard solution of sulfaquinoxaline respectively according to a concentration gradient of 0.5 to make the final concentration of sulfaquinoxaline Standard aqueous solution of 0.0, 0.5, 1.0, 1.5, 2.0, 2.5 mg / L respectively;

[0045] Step 2, the making of standard curve: take the standard aqueous solution whose final concentration of sulfaquinoxaline is 0.0mg / L in step 1 as the blank control, measure the respective absorbance values ​​with a UV spectrophotometer, and the UV wavelength is 210nm, and repeat it three times , find the average value, take different final concentrations as the abscissa, and take the corresponding absorbance averag...

Embodiment 3

[0048] Determination of Sulfaquinoxaline Content in Chinese Cabbage Contaminated by Sulfaquinoxaline in East Vegetable Garden of Xushui Township in Example 3

[0049] Chinese cabbage sample processing: take non-polluted Chinese cabbage and Chinese cabbage contaminated with sulfaquinoxaline in Dongcai Garden of Xushui Township, cut into strips, squeeze the juice, dilute 10 times and centrifuge, take the supernatant to prepare blank Chinese cabbage supernatant and Contamination of human cabbage supernatant.

[0050] The measurement steps are:

[0051] Step 1, prepare human cabbage supernatants with different final concentrations of sulfaquinoxaline: take 6 test tubes, add 5ml of blank Chinese cabbage supernatant to each, add sulfaquinoxaline standard solution to make the final concentration according to the concentration gradient of 0.5 0, 0.1, 0.5, 1.0, 1.5, 2.0 mg / L, shake the oscillator for 5 minutes, and rest for 10 minutes to obtain 6 Chinese cabbage supernatants with diff...

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Abstract

The invention discloses a method for quickly and quantitatively detecting sulfaquinoxaline residue. The method comprising the following steps: establishing by utilizing the feature that sulfaquinoxaline aqueous solution shows the stable absorption peak under the ultraviolet specific wavelength, wherein the content of the sulfaquinoxaline and an optical absorbance value thereof is in direct proportion, and the direct proportion variation trend is presented in a coordinate system, establishing a standard curve according to the coordinate system, and solving a regression equation Y=aX+b(b=0), and substituting an optical absorbance average value measured through the to-be-detected sample into the regression equation to compute the sulfaquinoxaline content of the sample, thereby reaching an aim of quickly and quantitatively detecting the sulfaquinoxaline residue.

Description

technical field [0001] The invention belongs to the technical field of drug detection, in particular to a method for rapid and quantitative detection of sulfaquinoxaline residues. Background technique [0002] Sulfaquinoxaline (suIfaquinoxaline) is called SQ for short, and its relative molecular mass is 300.4. Because farms add SQ to the feed or drinking water of animals during the process of raising animals, resulting in a large amount of SQ residues in food of animal origin. After people eat these animal-derived foods, it may cause SQ to accumulate in the human body, thereby causing allergies, allergies and other reactions in people, and even serious teratogenic and carcinogenic effects. For this reason, countries all over the world have formulated relatively strict standards for veterinary drug residues. At present, the residue standard of sulfonamides in food of animal origin in China is 300ng / g, while the residue standard set by the European Union, Japan, the United S...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 洪霞杜霞张淑雅
Owner ZHENJIANG YITE BIOTECH DEV CO LTD
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