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Application of tannic acid in calibration solution for electrospray ionization mass spectrum analysis

A technique of electrospray ionization and mass spectrometry, applied in the field of analytical chemistry mass spectrometry, can solve problems such as damage to the mass spectrometer detector, shorten the service life of the mass spectrometer, etc., and achieve the effects of simple preparation and accurate mass calibration

Active Publication Date: 2019-05-14
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, long-term use of high-concentration strong alkali as a calibration solution will damage the detector of the mass spectrometer and shorten the service life of the mass spectrometer

Method used

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  • Application of tannic acid in calibration solution for electrospray ionization mass spectrum analysis
  • Application of tannic acid in calibration solution for electrospray ionization mass spectrum analysis
  • Application of tannic acid in calibration solution for electrospray ionization mass spectrum analysis

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Experimental program
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Effect test

Embodiment 1

[0022] The solvent used for the sodium formate calibration solution in embodiment 1 is: to V 异丙醇 :V 水 =The mixed solvent formed after adding anhydrous formic acid to the solvent of 1:1, the formic acid content in this mixed solvent is 0.2v / v%;

[0023] N-benzylhexadecanamide was purchased from Wuhan Huashite Industrial Biotechnology Development Co., Ltd., analytically pure. β-cyclodextrin was purchased from Sigma Aldrich, analytically pure. Mono-[6-(diethylenetriamine)-6-deoxy]-β-cyclodextrin was purchased from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd.

[0024] The solvents used for N-benzylhexadecanamide solution, β-cyclodextrin solution and mono-[6-(diethylenetriamine)-6-deoxy]-β-cyclodextrin solution are: 乙醇 :V 水 =The mixed solvent formed after adding anhydrous formic acid to the solvent of 1:1, the formic acid content in this mixed solvent is 0.05v / v%.

[0025] The mass spectrometry conditions when using electrospray ionization mass spectrometry in Examples 1 a...

Embodiment 2

[0032] Example 2 Carry out electrospray ionization mass spectrometry analysis with tannic acid ethanol solution as calibration solution

[0033] In view of the advantages of the mass spectrogram of tannic acid obtained by positive ion scanning mass spectrometry in Example 1, the ethanol solution of tannic acid prepared in Example 1 was used as a calibration solution, and the concentration was analyzed by electrospray ionization mass spectrometry in positive ion mode. 1.0×10 -3 mol L -1 N-benzylhexadecanamide solution, the concentration is 1.0×10 -3 mol L -1 β-cyclodextrin solution and concentration of 1.0 x 10 -3 mol L -1 Mono-[6-(diethylenetriamine)-6-deoxy]--β-cyclodextrin solution. As a control, the above-mentioned N-benzylhexadecanamide solution, β-cyclodextrin solution and mono-[6-(diethylenetriamine)-6-deoxygenate were analyzed with the sodium formate calibration solution in Example 1. ]-β--cyclodextrin solution, the result is as Figure 2-4 shown.

[0034] Respe...

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Abstract

The present invention belongs to the mass spectrum analysis field in analytical chemistry, and specifically discloses an application of tannic acid in a calibration solution for electrospray ionization mass spectrum analysis. The present invention uses the tannic acid solution as an calibration solution for electrospray ionization mass spectrum analysis, and quickly realizes the accurate calibration of the molecular weight of a compound. The mass calibration is more accurate. Tannic acid is almost harmless to a mass spectrograph, especially a good result is obtained when tannic acid is used asthe calibration solution for electrospray ionization mass spectrometric detection of N-benzyl hexadecanamide, beta-cyclodextrin and mono-[6-(diethylenetriamino)-6-deoxy]-beta-cyclodextrin.

Description

technical field [0001] The invention relates to the application of tannic acid in electrospray ionization mass spectrometry calibration solution, and belongs to the field of analytical chemistry mass spectrometry. Background technique [0002] The rapid development of science and technology has put forward higher requirements for the accuracy of analysis and identification results, and the accurate detection of molecular weight plays a pivotal role in the analysis and identification of compounds. [0003] As an instrument that can give the molecular weight of a compound, a mass spectrometer plays a vital role in the fields of analysis, identification, and scientific research. Mass calibration is an indispensable part of mass spectrometry analysis of compounds, especially unknown compounds. [0004] Mass spectrometry commonly used calibration mass substances include sodium formate calibration solution, sodium trifluoroacetate calibration solution, polyvinyl alcohol, etc. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J49/00G01N27/62
Inventor 陈芮
Owner YUNNAN NORMAL UNIV
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