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Method for detecting various angiotensin II receptor blocker type hypotensive drugs in urine samples

A detection method and urine sample technology, which are applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome detection of sartan antihypertensive drugs, large amount of detection, cumbersome detection, etc., and achieve a simple and accurate detection method. High and simple detection method effect

Inactive Publication Date: 2019-05-24
苏州旭辉检测有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the detection of sartan antihypertensive drugs has always been cumbersome. The current detection method can only detect one antihypertensive drug at a time, resulting in a large amount of detection and cumbersome detection.

Method used

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  • Method for detecting various angiotensin II receptor blocker type hypotensive drugs in urine samples
  • Method for detecting various angiotensin II receptor blocker type hypotensive drugs in urine samples
  • Method for detecting various angiotensin II receptor blocker type hypotensive drugs in urine samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 provides a detection method of 6 kinds of sartan antihypertensive drugs in a urine sample, the detection method is as follows:

[0067] (1) Pretreatment of urine samples

[0068] Standard solution preparation: Altisartan / candesartan / irbesartan / losartan / olmesartan / telmisartan were dissolved in methanol to a drug concentration of 2.5 mg under ultrasonic conditions / mL, and then diluted to 0.5 μg / mL with methanol, as a standard solution.

[0069] Blank control preparation: Take 10 μL of methanol and add it to 190 μL blank urine sample, vortex for 1 min to prepare a blank control sample, take 100 μL of blank sample, add 200 μL of 2% formic acid aqueous solution, vortex for 1 min, then add 800 μL Methanol, vortexed for about 1 min, and then centrifuged for 5 min at a centrifugation speed of 13,000 rpm and a centrifugation temperature of 4°C, and then took 200 μL of the supernatant and injected it as a blank sample.

[0070] Urine sample preparation: Take 10 μL o...

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PUM

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Abstract

The invention discloses a method for detecting various angiotensin II receptor blocker type hypotensive drugs in urine samples. The method is characterized in that the angiotensin II receptor blockertype hypotensive drugs in urine are detected by means of liquid chromatograph-mass spectrograph joint use. The method includes steps of urine sample pretreatment, liquid chromatograph-mass spectrograph parameter optimization and urine sample detection. The defect that a detection method in the prior art fails to simultaneously detect the various angiotensin II receptor blocker type hypotensive drugs in the urine samples is overcome, and the simple and convenience detection method is provided for detecting various angiotensin II receptor blocker type hypotensive drugs in the urine samples. Thedetection method is convenient and simple in operation and high in accuracy, and whether the various angiotensin II receptor blocker type hypotensive drugs exist in urine or not can be judged simultaneously.

Description

technical field [0001] The invention relates to the field of urine sample detection, in particular to a method for detecting multiple sartan antihypertensive drugs in a urine sample. Background technique [0002] Sartan antihypertensive drugs are short for angiotensin II receptor antagonists. Angiotensin II has strong physiological activities, including: 1. Direct contraction of vascular smooth muscle; 2. Stimulation of the adrenal cortex to secrete aldosterone, which increases water and sodium retention; 3. Maintenance of sympathetic nerve activity. Sartans can block the combination of all tensin II produced by various pathways and its special receptors, thereby weakening or blocking its pressor effect. [0003] Sartan drugs have good organ protection effects on the heart, brain and kidney. A series of large-scale clinical trials have proved that when patients with heart failure, diabetes, kidney disease and left ventricular hypertrophy take this drug, it can not only redu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 李小川张枢黄晓林李玉杰王林花刘健袁小青聂巧刘达石海兵
Owner 苏州旭辉检测有限公司
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