Rapid quantitative test paper for type-O foot-and-mouth disease virus

A foot-and-mouth disease virus, quantitative detection technology, applied in the field of detection test paper, to achieve stable results, good specificity and repeatability, and high sensitivity

Inactive Publication Date: 2019-05-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Colloidal gold immunochromatographic test paper can only achieve qualitative or semi-quantitative detection through naked eye observation, so it is necessary to develop a rapid quantitative detection method for foot-and-mouth disease virus

Method used

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  • Rapid quantitative test paper for type-O foot-and-mouth disease virus
  • Rapid quantitative test paper for type-O foot-and-mouth disease virus
  • Rapid quantitative test paper for type-O foot-and-mouth disease virus

Examples

Experimental program
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Embodiment Construction

[0024] The following are specific embodiments of the present invention.

[0025] 1. Preparation of O-type foot-and-mouth disease virus VLPs

[0026] 1) Expression of recombinant VLPs protein

[0027] Inoculate competent cells BL21(DE3)-RIL expressing bacteria co-transformed with positive recombinant plasmids pSMK-VP0, pSMK-VP3 and pSMA-VP1 preserved by our laboratory into 10μg / mL Kanamyces at a ratio of 1:100 In fresh LB liquid medium sterilized by autoclaving, 50 μg / mL ampicillin and 25 μg / mL chloramphenicol, cultivate overnight at 37 °C on a shaker at 220 r / min. Inoculate the overnight cultured expression bacteria into fresh autoclaved LB liquid medium containing 10 μg / mL kanamycin, 50 μg / mL ampicillin and 25 μg / mL chloramphenicol, and place on a shaker at 37 °C and 220 r / min. Shake until the OD600 value of the bacterial solution is about 0.8. Add IPTG (isopropyl-β-D-thiogalactopyranoside) at a final concentration of 0.5mmol / L, and induce at 16°C for 16h to collect the ba...

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Abstract

The invention discloses rapid quantitative test paper for a type-O foot-and-mouth disease virus. The test paper comprises a bottom plate, a sample pad, a conjugate pad, an analysis membrane and a water absorption pad. The bottom plate is made of a waterproof material, and the sample pad, the conjugate pad, the analysis membrane formed by a nitrocellulose membrane and the water absorption pad madeof a water absorbing material are sequentially fixed to the bottom plate made of the waterproof material. A rare earth luminescent material and staphylococcus aureus A protein coupled conjugate is added to the conjugate pad, and the nitrocellulose membrane is provided with a detection line made of type-O foot-and-mouth disease virus-like particles and a control line made of rabbit IgG. By adoptionof the type-O foot-and-mouth disease virus-like particles as capture antigens, rapid quantitative testing of the type-O foot-and-mouth disease virus antibody level in serum can be realized, high immunogenicity is achieved while threats of virus shedding are avoided, and safety and harmlessness are guaranteed for operators and the environment.

Description

technical field [0001] The invention relates to a detection test paper for rapid and quantitative detection of O-type foot-and-mouth disease virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, febrile, highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV). There are 7 serotypes of foot-and-mouth disease virus, A, O, C (European type), SAT1, SAT2, SAT3 (African type) and Asia I (Asian type, etc.). In the long-term evolution process, many mutant strains have been produced. At present, there are more than 80 subtypes. The antigenicity of different subtypes or isolates within the same serotype is different to varying degrees, and the degree of serological cross-reactivity is also different. Not the same, this feature brings great difficulties to the diagnosis and control of foot-and-mouth disease. To fundamentally control the occurrence and prevalence of foot-and-mouth disease, we must first strengthen the research on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/533
Inventor 郭慧琛侯凤萍张韵孙世琪张禹白满元茹嘉喜靳野郭建宏殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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