Rapid quantitative test paper for type-O foot-and-mouth disease virus
A foot-and-mouth disease virus, quantitative detection technology, applied in the field of detection test paper, to achieve stable results, good specificity and repeatability, and high sensitivity
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[0024] The following are specific embodiments of the present invention.
[0025] 1. Preparation of O-type foot-and-mouth disease virus VLPs
[0026] 1) Expression of recombinant VLPs protein
[0027] Inoculate competent cells BL21(DE3)-RIL expressing bacteria co-transformed with positive recombinant plasmids pSMK-VP0, pSMK-VP3 and pSMA-VP1 preserved by our laboratory into 10μg / mL Kanamyces at a ratio of 1:100 In fresh LB liquid medium sterilized by autoclaving, 50 μg / mL ampicillin and 25 μg / mL chloramphenicol, cultivate overnight at 37 °C on a shaker at 220 r / min. Inoculate the overnight cultured expression bacteria into fresh autoclaved LB liquid medium containing 10 μg / mL kanamycin, 50 μg / mL ampicillin and 25 μg / mL chloramphenicol, and place on a shaker at 37 °C and 220 r / min. Shake until the OD600 value of the bacterial solution is about 0.8. Add IPTG (isopropyl-β-D-thiogalactopyranoside) at a final concentration of 0.5mmol / L, and induce at 16°C for 16h to collect the ba...
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