Microbial immobilization agent for increasing available nitrogen of tea garden soil and preparation method thereof

A microbial immobilization and soil technology, applied in the direction of immobilization on or in inorganic carriers, on/in organic carriers, organic fertilizers, etc., can solve the practical application effect of nitrogen-limiting transformation bacteria, nitrogen transformation Bacterial growth stress, easy loss of microorganisms and other problems, to achieve the effect of improving stress resistance, increasing biological density, and achieving remarkable results

Inactive Publication Date: 2019-05-31
TEA RES INST ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical application, since the tea garden soil environment is rich in high active aluminum, it will cause growth stress to most nitrogen transforming bacteria, and at the same t

Method used

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  • Microbial immobilization agent for increasing available nitrogen of tea garden soil and preparation method thereof
  • Microbial immobilization agent for increasing available nitrogen of tea garden soil and preparation method thereof
  • Microbial immobilization agent for increasing available nitrogen of tea garden soil and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0025] The preparation steps of the microorganism immobilizing agent used in embodiment 1 are as follows:

[0026] 1) Acquisition of nitrogen-converting bacteria: Take 10 g of soil sample and put it into an Erlenmeyer flask, add 90 mL of sterile water to the Erlenmeyer flask for dilution, and the sterile water contains 100 mM A l3+ , and shake overnight to obtain a soil suspension. Use a 1mL sterile pipette to draw the above soil suspension into a 9mL sterile water test tube for dilution, and dilute in this way to obtain 10 2 、10 3 and 10 4 double dilution. Take 100 μL of the above 10 2 、10 3 and 10 4 Spread the two-fold dilution on casein solid medium, culture at 28°C for 3 days, isolate and purify the strains with better growth, transfer them to Asobi nitrogen-free medium, and culture at 28°C for 3 days, select the strains with better growth strains are nitrogen transforming bacteria.

[0027] 2) Cultivation of nitrogen-converting bacteria: inoculate the nitrogen-con...

Embodiment 2

[0040] The preparation steps of the microorganism immobilizing agent used in embodiment 2 are as follows:

[0041] 1) Acquisition of nitrogen-converting bacteria: Take 10 g of soil sample and put it into an Erlenmeyer flask, add 90 mL of sterile water to the Erlenmeyer flask for dilution, and the sterile water contains 100 mM A l3+ , and shake overnight to obtain a soil suspension. Use a 1mL sterile pipette to draw the above soil suspension into a 9mL sterile water test tube for dilution, and dilute in this way to obtain 10 2 、10 3 and 10 4 double dilution. Take 100 μL of the above 10 2 、10 3 and 10 4 Spread the two-fold dilution on casein solid medium, culture at 28°C for 4 days, isolate and purify the strains that grow better, transfer them to Asobi nitrogen-free medium, and culture at 28°C for 4 days, select the strain that grows better strains are nitrogen transforming bacteria.

[0042] 2) Cultivation of nitrogen-converting bacteria: inoculate the nitrogen-convert...

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Abstract

The invention belongs to the technical field of agricultural intensive production and particularly relates to a microbial immobilization agent capable of improving available nitrogen of tea garden soil and a preparation method thereof. The microbial immobilization agent comprises nitrogen transformation bacteria, an adsorption material and an embedding agent, wherein the mass ratio of the nitrogentransformation bacteria to the adsorption material to the embedding agent is 8: 1: 400 in sequence, and the microbial immobilization agent contains 1.0*107-1.0*108 cfu/g of viable bacteria. The microbial immobilization agent can effectively convert nitrogen in soil and even air into effective nitrogen which can be absorbed by tea trees, so that the application amount of chemical fertilizers in tea gardens can be effectively reduced, and the quality of tea leaves can be ensured. The invention further aims to provide a preparation method of the microbial immobilization agent so as to conveniently and efficiently realize accurate preparation of the microbial immobilization agent. The preparation method comprises the steps of obtaining nitrogen transformation bacteria, culturing the nitrogentransformation bacteria, preparing an embedding agent and preparing a microbial immobilization agent.

Description

technical field [0001] The invention belongs to the technical field of agricultural intensive production, and in particular relates to a microorganism immobilizing agent capable of increasing available nitrogen in tea garden soil and a preparation method thereof. Background technique [0002] Tea tree is one of the main leaf-using economic crops in my country, and the annual leaf harvesting will take away a large amount of nitrogen-containing nutrients. Due to the low nutrient content of organic fertilizers, there are few effective nutrients in the short term, and it is difficult to meet the needs of crop growth in the current season. Therefore, in order to maintain production, tea farmers can only apply a large amount of chemical nitrogen fertilizer to the tea garden every year. However, the long-term application of chemical fertilizers in tea gardens can easily cause soil compaction, increase in soil-borne diseases, decline in tea quality, and aggravation of agricultural ...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/04C05G3/04C05F11/08C05G3/80
Inventor 罗毅王烨军孙宇龙夏先江张永利苏有健廖珺
Owner TEA RES INST ANHUI ACAD OF AGRI SCI
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