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Intelligent-sampling pauci-molecular genetic sequence detection method and device

A technology of sequence detection and number of molecules, which is applied in the field of genetic sequence detection of a small number of molecules by intelligent sampling, can solve the problems of inconvenient upgrade and correction, biased errors, etc., achieve good application adaptability and input sensitivity, reduce system costs, Avoid the effects of the PCR process

Inactive Publication Date: 2019-05-31
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current genetic sequence detection process requires doubling of the original molecule and other preparatory processes, bias errors caused by DNA chain amplification, and inconvenient upgrades and corrections

Method used

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  • Intelligent-sampling pauci-molecular genetic sequence detection method and device
  • Intelligent-sampling pauci-molecular genetic sequence detection method and device
  • Intelligent-sampling pauci-molecular genetic sequence detection method and device

Examples

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example 1

[0054] List the parameters of this embodiment 1 processing data here:

[0055] Step (1) The number of crystallite particles is 2000-10000 per square micron, and the distance between crystallite particles is greater than 0.5nm and less than 15nm;

[0056] Step (2) using a strong electric field to drive a weak magnetic field to focus, and controlling the DNA chain of the genetic information carrier to flow along the direction of the small hole at a speed of 5 microns to 5 mm per second;

[0057] Step (3) adopts common desktop computer, and the throughput of data is between 1M / s to 8M / s;

[0058] Step (4) by controlling the flow rate, the total curvature of the genetic information carrier deoxyribonucleic acid molecule is within 40 degrees;

[0059] Step (5) Obtain the system response matrix by means of experiment and simulation. After 5 independent tests, the error rate is required to be below one part per million;

[0060] Step (6) adopts an analytical method for solving large-...

example 2

[0062] The parameters for processing data in this application example 2 are listed here:

[0063] Step (1) The number of crystallite particles is 200-50000 per square micron, and the distance between crystallite particles is greater than 0.1nm and less than 15nm;

[0064] Step (2) using a strong electric field to drive a weak magnetic field to focus, and controlling the DNA chain of the genetic information carrier to flow along the direction of the small hole at a speed of 50 microns to 10 mm per second;

[0065] Step (3) using an ordinary smart phone as a data access carrier, which can be published on the Internet, and the data throughput is between 100k / s and 800k / s;

[0066] Step (4) by controlling the flow rate, the total curvature of the genetic information carrier deoxyribonucleic acid molecule is within 30 degrees;

[0067] Step (5) Obtain the system response matrix by means of experiment and simulation. After 500 independent tests, the error rate is required to be bel...

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Abstract

The invention discloses an intelligent-sampling pauci-molecular genetic sequence detection method and device, belonging to the field of genetic information carrier deoxyribonucleic acid detection. Theintelligent-sampling pauci-molecular genetic sequence detection method includes the following steps: S1, epitaxially growing scattered microcrystalline particles on a substrate of a scintillation crystal, attaching a polyethylene film to the substrate of the scintillation crystal, and epitaxially growing a diode array on the polyethylene film; S2, directing the genetic information carrier deoxyribonucleic acid molecule into the gap between the polyethylene film and the substrate of the scintillation crystal; S3, acquiring electron distribution by the diode array, and outputting the result toa computer; S4, reconstructing position information; S5, testing system response matrix; and S6, calculating a vector consisting of nucleotide species. The intelligent-sampling pauci-molecular geneticsequence detection method and device have better application adaptability and input sensitivity, can reduce the system cost, and have good modular features.

Description

technical field [0001] The invention belongs to the fields of genetic information carrier deoxyribonucleic acid detection, digital sampling technology, biological information and biomedical detection, and in particular relates to an intelligent sampling method and device for detecting a small number of genetic sequences. Background technique [0002] Genetic information carrier deoxyribonucleic acid chain sequence detection refers to the analysis of the base sequence of a specific genetic information carrier deoxyribonucleic acid chain fragment, that is, the arrangement of adenine, thymine, cytosine and guanine. The rapid detection method of genetic information carrier deoxyribonucleic acid chain sequence has greatly promoted the research and discovery of biology and medicine. [0003] In basic biological research, and in numerous application fields, such as diagnosis, biotechnology, forensic biology, and biosystematics, the knowledge of the DNA chain sequence of the genetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/00
Inventor 邓玉姗邓宏晟赵欣邓贞宙刘志涛段志文韩春雷
Owner NANCHANG UNIV
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