Application of xanthohumol and derivatives thereof in preventing or treating neurogenerative disease

A neurodegenerative, xanthohumol technology used in the field of medicine

Inactive Publication Date: 2019-07-09
深圳夏浠湾医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of xanthohumol and its derivatives in the prevention or treatment of neurodegenerative diseases has not been reported

Method used

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  • Application of xanthohumol and derivatives thereof in preventing or treating neurogenerative disease
  • Application of xanthohumol and derivatives thereof in preventing or treating neurogenerative disease
  • Application of xanthohumol and derivatives thereof in preventing or treating neurogenerative disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Effects of xanthohumol and its derivatives on the viability of N2a cells.

[0028] method:

[0029] Take out N2a / WT cells (wild type) from liquid nitrogen and immediately soak them in a 37°C water bath, centrifuge at 600r / min for 5min after melting, absorb the supernatant, add 1mL sterile PBS to wash, centrifuge again, and remove the supernatant , add 1mL of culture medium, inoculate into a petri dish after pipetting evenly, and place at 37°C, 5% CO 2 Cultivate for 24 hours under the environment; when the cells adhere to the wall and grow to 60%, digest with 0.25% trypsin for 2 minutes, remove the supernatant, add an appropriate amount of medium and pipette evenly, with 1×10 4 / ml density was seeded on a 96-well plate, and the cells in the logarithmic growth phase were used for the experiment.

[0030] Take another 96-well plate, add 100 μl DMEM medium to each well, and add 1×10 4 N2a / WT cells were inoculated at a density of 1 / ml and placed at 37°C, 5% CO 2 When cul...

Embodiment 2

[0039] Effects of Xanthohumol and Its Derivatives on Inhibiting Aβ Aggregation

[0040] Alzheimer's disease (AD) is a neurodegenerative disease accompanied by cognitive impairment in the whole brain, including decline in memory, sense of direction, judgment and reasoning ability. How to effectively inhibit the production of Aβ aggregation and neurotoxicity is the best treatment One of the possible effective ways of AD, the following study on the effect of xanthohumol and its derivatives on the inhibition of Aβ aggregation.

[0041] method:

[0042] Thaw N2a / WT cells and N2a / APP cells from liquid nitrogen quickly in a 37°C water bath, place them in a 6cm culture dish after thawing, and use 10mL medium (DMEM containing 10% FBS and 1% double antibody Medium), placed in CO 2 Cell incubator (5% CO 2 , 37°C) for 24 hours, and replace the fresh medium every 24 hours; when the cells adhere to the wall and grow to more than 70%, digest with 1mL 0.25% trypsin for 1min, absorb the try...

Embodiment 3

[0057] CDK (Cycle Dependent Kinase) is a family of serine / threonine kinases that regulate the progression of the cell cycle after being activated by cyclins, while CDK-5 does not participate in the regulation of the cell cycle, and its expression is distributed in tissues throughout the body, especially in the nerves The most abundant system, CDK-5 may be an upstream sensor of neuronal death. CDK-5 is anchored to the membrane under the signal action of p35, p39 and other regulatory subunits, phosphorylates membrane substrates, and plays a role in neuron development, migration, and neuron development by balancing pro-apoptotic and pro-survival signal transduction pathways. important role in survival. Under the stimulation of Aβ, p35 can be cleaved into p25 through the action of calpain. In this example, a method similar to that of Example 2 is used to carry out Xn via p25 / p35 inhibits the phosphorylation of tau protein, so as to investigate the potential activity of Xn in the...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to application of xanthohumol and derivatives thereof in preparing drug for preventing or treating neurogenerative disease, especially for Alzheimer's disease. The inventor verifies that xanthohumol and the derivatives can remarkably lower Abeta1-40/42 concentration to inhibit Abeta gathering, can inhibit Tau protein phosphorylation by inhibiting CDK-5 activity and can serve as drug for treating potential neurogenerative disease like Alzheimer's disease to be further developed and utilized through Alzheimer's disease transgenic cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the use of xanthohumol and its derivatives in preventing or treating neurodegenerative diseases. Background technique [0002] Xanthohumol is a prenyl flavonoid compound, which is a unique substance in hops. At present, xanthohumol is mainly extracted from hops. For example, it can be obtained by macroporous resin adsorption method, organic solvent extraction method, Ultrasonic technology extraction method, chromatographic extraction method and their combination are extracted from hops. Xanthohumol has unique health attributes. At present, the pharmacological activity of xanthohumol has been studied. Its main function is anti-cancer effect, mainly It is manifested in achieving the preventive effect by regulating the pathway of tumor formation and inhibiting the proliferation of cancer cells; at the same time, it has also been found that xanthohumol has the effect of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/11A61K31/222A61P25/28A61P25/16
CPCA61K31/11A61K31/222
Inventor 夏俊霞齐菲张在军王亮
Owner 深圳夏浠湾医药科技有限公司
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