Preparation method of molecularly imprinted ratio fluorescence probe and method for detecting oxytetracycline by means of same

A ratiometric fluorescent probe, molecular imprinting technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measurement devices, etc., can solve the problems of high cost, long detection time, oxytetracycline susceptible to environmental interference, etc., and achieve environmental protection of materials. , short detection time, anti-interference and high selectivity

Active Publication Date: 2019-07-12
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of high cost of the synthetic method of ratiometric fluorescent material and the problem of being susceptible to environmental interference and long detection time in the detection process of oxytetracycline, and to provide a method for preparing a molecularly imprinted ratiometric fluorescent probe and using it to detect oxytetracycline prime method

Method used

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  • Preparation method of molecularly imprinted ratio fluorescence probe and method for detecting oxytetracycline by means of same
  • Preparation method of molecularly imprinted ratio fluorescence probe and method for detecting oxytetracycline by means of same
  • Preparation method of molecularly imprinted ratio fluorescence probe and method for detecting oxytetracycline by means of same

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specific Embodiment approach 1

[0017] Embodiment 1: This embodiment is a method for preparing a molecularly imprinted ratiometric fluorescent probe, which is specifically prepared according to the following steps:

[0018] 1. Preparation of carbon quantum dots: Add passion fruit peel and urea into high-purity water, then place them in a microwave digestion tank, start the microwave digestion instrument, set the reaction time to 25min-35min, and the temperature to 180°C-220°C, the reaction is complete Finally, use a disposable syringe and a microporous filter membrane to filter to obtain a carbon quantum dot solution. The carbon quantum dot solution is removed by rotary evaporation at a temperature of 40°C to 70°C to remove the solvent, and then vacuum dried at a temperature of 50°C to 70°C for 20 hours. ~30h, obtain carbon quantum dot solid product; The mass ratio of urea described in step 1 and passion fruit peel is 1:(5~7); The mass ratio of passion fruit peel described in step 1 and high-purity water is 1...

specific Embodiment approach 2

[0021] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the pore size of the microporous filtration membrane in Step 1 is 0.22 μm. Others are the same as the first embodiment.

specific Embodiment approach 3

[0022] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is: in step 2, stir and mix with magnetic force at a stirring speed of 200 rpm to 300 rpm for 20 min to 30 min at room temperature. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a preparation method of a molecularly imprinted ratio fluorescence probe and a method for detecting oxytetracycline by means of the same. The invention aims to solve the problem that a synthetic method of a ratio fluorescence material is high in cost, is easily interfered by the environment in the oxytetracycline detection process and is long in detection time. The preparation method comprises the following steps: 1, preparing carbon quantum dots; 2, preparing a mesoporous molecularly imprinted polymer; and 3, preparing the molecularly imprinted ratio fluorescence probe. The method for detecting oxytetracycline comprises the following steps: 1, preparing a dispersion liquid; 2, determining a quenching constant Ksv; and 3, detecting a to-be-detected sample, and calculating concentration Qu of oxytetracycline in the to-be-detected sample according to a Stern-Volmer equation. The preparation method has the advantages that the preparation method is short in detection time, has a good recovery rate and reproductivity and has relatively high selectivity to oxytetracycline drugs. The preparation method provided by the invention is primarily used for preparing themolecularly imprinted ratio fluorescence probe and detecting oxytetracycline.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted ratio fluorescent probe and a method for detecting oxytetracycline. Background technique [0002] Oxytetracycline is a tetracycline antibiotic with good antibacterial and bactericidal effects. As a broad-spectrum antibiotic, it is widely used in aquaculture and animal husbandry. However, because oxytetracycline is widely used and abused, the content of oxytetracycline in some animal foods, ground water and production wastewater often exceeds the standard. Low concentrations of such drugs remain in animal foods, causing various bacteria to develop drug resistance and seriously threatening human health. The existing detection method of oxytetracycline is mainly high performance liquid chromatography, which has high equipment cost, complicated pretreatment and long detection period. In recent years, some researchers have also used fluorescence analysis to detect oxytetracycline. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/85G01N21/64
CPCC09K11/06C09K11/7732G01N21/6428G01N2021/6432
Inventor 陈立钢孙雪孙晓琪
Owner NORTHEAST FORESTRY UNIVERSITY
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