Disease-resistant gene and protein for controlling plant evasion aphid resistance character and application thereof
A disease-resistant gene and protein technology, applied in the field of bioengineering, can solve the problems of no disease-resistant gene and no positioning
Active Publication Date: 2019-07-12
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology
[0011] At present, although the genetic analysis of the aphid resistance traits derived from Shouxing peach has preliminarily determined the position of the aphid resistance gene derived from Shouxing peach, it has not been accurately located, nor has the disease resistance that controls the aphid resistance traits of plants been found. Gene
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Embodiment 1
[0050] The nucleotide sequence of the new disease-resistant gene controlling the traits of aphid resistance and avoidance of plants in this example is shown in SEQ ID NO.2.
[0051] Example 1 of the protein controlling plant avoidance and aphid resistance traits
[0052] The amino acid sequence of the protein controlling aphid-resistance traits of plants in this example is shown in SEQ ID NO.3.
[0053] Example 1 of the Application of the Disease Resistance Gene Controlling Plant Avoidance and Aphid Resistant Characters in the Screening of Aphid Resistance Individual Plants
[0054] In this embodiment, the disease-resistant gene is used as a molecular marker to detect whether the disease-resistant gene exists, and a single plant with the disease-resistant gene can be determined to be an aphid-resistant individual plant, thereby accurately screening out an aphid-resistant individual plant.
[0055] The ways to detect the disease-resistant gene include: designing specific prime...
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The invention relates to a disease-resistant gene and protein for controlling plant evasion aphid resistance character and an application thereof, and belongs to the technical field of biological engineering. By the adoption of the disease-resistant gene and protein for controlling the plant evasion aphid resistance character and the application thereof, a candidate gene is boldly predicted according to a gene function and a re-sequencing result, a specific upstream primer is designed by inserting a 20bp sequence at 400bp upstream of an initiation codon of the disease-resistant gene (ppa000596m), a downstream primer is designed by a downstream conservative region of a termination codon of the ppa000596m gene, and the disease-resistant gene which is completely absent in a peach genome is obtained by amplification. The existence of the gene is coseparated from the aphid resistance character, and an expression mode of a new gene is further analyzed, the result shows that the expression ofthe gene in a group is consistent with the aphid resistance of a single plant, and the new gene is a new disease-resistant gene for controlling peach tree evation aphid resistance character. The geneand the encoded protein thereof can be applied to multiple aspects such as aphid-resistant single plant screening, plant aphid-resistant character enhancement, peach aphid-resistant variety breedingand the like.
Description
technical field [0001] The invention relates to a disease-resistant gene and protein for controlling aphid-resistance traits of plants and an application thereof, belonging to the technical field of bioengineering. Background technique [0002] Peach is an important economic tree species in my country. According to the statistics of the Food and Agriculture Organization of the United Nations, in 2014, my country’s peach planting area was 728,400 hectares, with a total output of 12.45 million tons, accounting for 48.7% and 54.6% of the world’s total respectively, ranking first in the world. Aphids are one of the main pests that affect the growth of peach trees. There are 3 species of aphids that harm peach trees, green peach aphid, green peach aphid, and green peach aphid. It is also a piercing-sucking pest widely distributed in the world. Aphids use piercing-sucking mouthparts to suck juice from young plant tissues, causing plant nutrition loss, stunted growth, growth defor...
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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8286
Inventor 潘磊王志强牛良樊美丽鲁振华曾文芳崔国朝
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI