A method for simultaneously determining the content of multiple chemical components in Sanjiapi medicinal material
A technology of chemical composition and Sanjiapi, which is applied in the direction of measuring devices, material separation, scientific instruments, etc., can solve the problems of inability to guarantee the safety and effectiveness of clinical medication, difficulty in controlling the quality of medicinal materials and their formulations, and restrictions on Sanjiapi. Problems such as the development and utilization of medicinal materials, to achieve the effect of improving safety and stability, high accuracy, and simple detection methods
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[0037] A method for simultaneously determining the contents of multiple chemical components in Sanjiapi medicinal materials, the method is to simultaneously measure chlorogenic acid, teretin, and isochlorogenic acid A contained in Sanjiapi medicinal materials under the same chromatographic condition. And 4 kinds of chemical components of isochlorogenic acid C carry out liquid chromatography content determination, and concrete method comprises the following steps:
[0038] (1) Chromatographic conditions:
[0039] Phenomenex Gemini C18 chromatographic column, 250 mm×4.6mm, 5 μm, mobile phase is acetonitrile-0.1% phosphoric acid aqueous solution by volume, acetonitrile is used as mobile phase A, and phosphoric acid aqueous solution with 0.1% volume percentage is used as For mobile phase B, perform gradient elution according to the gradient elution method in Table 1: injection volume: 5 μL; flow rate: 1 mL / min; column temperature: 30 °C; detection wavelength: 325 nm;
[0040] ...
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