A method for simultaneously determining the content of multiple chemical components in Sanjiapi medicinal material

A technology of chemical composition and Sanjiapi, which is applied in the direction of measuring devices, material separation, scientific instruments, etc., can solve the problems of inability to guarantee the safety and effectiveness of clinical medication, difficulty in controlling the quality of medicinal materials and their formulations, and restrictions on Sanjiapi. Problems such as the development and utilization of medicinal materials, to achieve the effect of improving safety and stability, high accuracy, and simple detection methods

Active Publication Date: 2022-03-11
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

At present, there are no research reports on the content determination methods of various chemical components contained in Sanjiapi medicinal materials, which limits the further development and utilization of Sanjiapi medicinal materials
In addition, there is no quantitative detection of its medicinal ingredients, it is difficult to control the quality of medicinal materials and their prescriptions, and it is impossible to guarantee the safety and effectiveness of clinical medication

Method used

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  • A method for simultaneously determining the content of multiple chemical components in Sanjiapi medicinal material
  • A method for simultaneously determining the content of multiple chemical components in Sanjiapi medicinal material
  • A method for simultaneously determining the content of multiple chemical components in Sanjiapi medicinal material

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Embodiment 1

[0037] A method for simultaneously determining the contents of multiple chemical components in Sanjiapi medicinal materials, the method is to simultaneously measure chlorogenic acid, teretin, and isochlorogenic acid A contained in Sanjiapi medicinal materials under the same chromatographic condition. And 4 kinds of chemical components of isochlorogenic acid C carry out liquid chromatography content determination, and concrete method comprises the following steps:

[0038] (1) Chromatographic conditions:

[0039] Phenomenex Gemini C18 chromatographic column, 250 mm×4.6mm, 5 μm, mobile phase is acetonitrile-0.1% phosphoric acid aqueous solution by volume, acetonitrile is used as mobile phase A, and phosphoric acid aqueous solution with 0.1% volume percentage is used as For mobile phase B, perform gradient elution according to the gradient elution method in Table 1: injection volume: 5 μL; flow rate: 1 mL / min; column temperature: 30 °C; detection wavelength: 325 nm;

[0040] ...

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Abstract

The invention belongs to the technical field of detection of traditional Chinese medicinal materials, and in particular relates to a method for simultaneously determining the contents of various chemical components in Sanjiapi medicinal materials, including (1) chromatographic conditions, (2) preparation of a reference solution, and (3) preparation of a test solution Preparation (4) Determination and other steps, the present invention provides a method for simultaneously determining the contents of various chemical components in Sanjiapi medicinal materials, which can simultaneously determine chlorogenic acid, auretin, and isochlorogenic acid A in Sanjiapi medicinal materials As well as the content of the four chemical components of isochlorogenic acid C, the detection method is simple, high in accuracy, good in stability and good in reproducibility, and can more comprehensively and accurately evaluate the quality of Sanjiapi, which is beneficial to the investigation of the medicinal material. Effective quality control can provide a basis for comprehensive evaluation and control of the quality of Sanjiapi medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicinal materials, and in particular relates to a method for simultaneously measuring the contents of various chemical components in Sanjiapi medicinal materials. Background technique [0002] Sanjiapi, also known as Baiyugen, Cisanjia, Fengdangyu, etc., is the root or root bark of Araliaceae plant Baizhu. The Latin name of the plant is: Acanthopanax trifoliatus (L.) Merr. [ Zanthaxylum trifoliatum L. ]. Bougainvillea is a climbing shrub, 1-7m high, thin and weak branches, old branches grayish white, new branches brownish yellow, sparsely downward needles, spines hooked at the apex, and flat at the base. Alternate leaves, thorns or no thorns, leaves oval-ovate to oval-oblong, drupes berry-shaped, oblate, black when mature; flowering period from August to November, fruit period from September to December. Sanjiapi is mainly produced in Guangdong, Guangxi, Yunnan, Sich...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 蒋林农毅清周雅琪李芳婵郭勇秀随家宁魏博伟
Owner GUANGXI UNIV OF CHINESE MEDICINE
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