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Rapid detection box and method for rapidly detecting medicinal materials using the same

A detection box, fast technology, applied in the fields of medicine, medical detection, and food, can solve problems such as catheter blockage, achieve high safety, improve efficiency and accuracy, and fast detection speed

Active Publication Date: 2022-02-01
BEIJING INST FOR DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet in the method that the researcher of this application is used for the aflatoxin in the rapid detection medicinal material by using this rapid detection box, but faces new problem that needs to be solved, for example, when using some extraction solvents, it is easy to cause catheter blockage

Method used

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  • Rapid detection box and method for rapidly detecting medicinal materials using the same
  • Rapid detection box and method for rapidly detecting medicinal materials using the same
  • Rapid detection box and method for rapidly detecting medicinal materials using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment 1: the method for detecting the total amount of aflatoxin in medicinal materials

[0103] (1) provide colloidal gold test paper card for detecting aflatoxin (this embodiment directly applies the commercialized ROSA FAST5 aflatoxin quantitative rapid detection strip, article number LF-AFQ-FAST5-100K, brand bioshome); the LF-AFQ-FAST5-100K includes: sample pad, nitrocellulose membrane (NC membrane), absorbent pad and PVC back lining; the nitrocellulose membrane is adhered to the PVC backing, and the sample pad and the absorbent pad are respectively adhered to the two ends of the nitrocellulose membrane; the sample pad, detection line T1, detection line T2, quality control line are distributed on the nitrocellulose membrane in sequence. line, absorbent pad;

[0104] (2) Sample extraction: Take 1 gram by weight of the powder of the medicinal material to be tested or its decoction pieces, add 5 ml of acetonitrile by volume, ultrasonically oscillate for 4 minutes...

Embodiment 2

[0118] Embodiment 2: detect the method for aflatoxin total amount in medicinal material

[0119] (1) Provide colloidal gold test strips for the detection of aflatoxins;

[0120] (2) Sample extraction: Take 1 gram by weight of the powder of the medicinal material or its decoction pieces to be tested, add 1 ml of acetonitrile by volume, oscillate ultrasonically for 5 minutes, centrifuge at 2500 rpm for 10 minutes, take an appropriate amount of supernatant, and add to 50 times the amount of dilution buffer solution (a phosphate buffer solution with a phosphate ion concentration of 10 mmol / L and a pH value of 6.5) to obtain a sample extract, to be detected;

[0121] (3) Sample determination: pipette 100 μl of the sample extract obtained in step (2), point it on the colloidal gold test paper card (the part of the sample pad), incubate at a constant temperature of 45°C for 4 minutes, and use a reader to measure the reading of the test strip , and calculate the aflatoxin content in ...

Embodiment 3

[0123] Embodiment 3: the method for detecting the total amount of aflatoxin in medicinal materials

[0124] (1) Provide colloidal gold test strips for the detection of aflatoxins;

[0125] (2) Sample extraction: Take 1 gram by weight of the powder of the medicinal material to be tested or its decoction pieces, add 8 ml of acetonitrile by volume, oscillate ultrasonically for 2 minutes, and centrifuge at 5000 rpm for 2 minutes, take an appropriate amount of supernatant, add to 5 times the amount of dilution buffer solution (a phosphate buffer solution with a phosphate ion concentration of 25mmol / L and a pH value of 7.5) to obtain a sample extract, to be detected;

[0126] (3) Sample determination: pipette 300 μl of the sample extract obtained in step (2), point it on the colloidal gold test paper card (the part of the sample pad), incubate at a constant temperature of 45°C for 6 minutes, and use a reader to read the test strip. , and calculate the aflatoxin content in medicinal...

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Abstract

The application relates to a rapid detection box and a method for using it to quickly detect medicinal materials, including a box body and a box cover connected by a first hinge, and matching locks; four corners of the bottom surface of the detection box are respectively provided with feet; the box body and the inner side of the box cover are provided with a storage board, and a number of test article limit slots are arranged in the storage board; a second hinge is also provided on the box cover to connect the operating table that can be opened and retracted, and it is in a horizontal state when it is opened; The inside of the body and the box cover is also provided with hinged support rods connected to each other, so that the box cover and the box body placed horizontally are basically vertical. It also relates to a method for the rapid detection box to detect medicinal materials, especially aflatoxin therein. The application of the rapid detection box combined with the detection method has the advantages of fast detection speed, high sensitivity, high safety, low cost and low professional requirements for operators, etc. It can be applied to the residue detection in the laboratory and on-site, which is conducive to improving food and drug safety. Efficiency and accuracy of inspection work.

Description

technical field [0001] This application belongs to the technical field of medicine, food, etc., especially the detection technology field of medicine, food, etc., and relates to a rapid detection of related substances in materials (such as medicine, food, etc., especially Chinese medicinal materials), such as the detection of Rapid test kit for aflatoxin. Background technique [0002] Medicine and food safety testing are playing an increasingly important role in ensuring food safety. In the existing technology, the detection of medicine and food safety mainly relies on laboratory analysis and detection technology of large instruments such as mass spectrometry and chromatography, but it has the disadvantages of large size and inconvenient portability, making it difficult to monitor food in a timely, fast, anytime, anywhere Safety status, not suitable for screening of a large number of samples. [0003] In order to facilitate the real-time detection of medicines and foods on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/58G01N33/558G01N1/28
CPCG01N33/58G01N33/558G01N1/28
Inventor 傅欣彤范妙璇杨文良郭洪祝陈有根陈思妮梅婕魏叶
Owner BEIJING INST FOR DRUG CONTROL