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Preparation method of ferrocene-based covalent organic framework modified electrode and method for electrochemical detection of troponin

A covalent organic framework and modified electrode technology, applied in the field of electrochemical sensing, can solve the problems of difficult to meet the requirements of high sensitivity and high selectivity detection of markers, complex components, low content, etc., to achieve easy modification and adsorption, Clear pore size distribution and large specific surface area

Active Publication Date: 2021-10-26
MINNAN NORMAL UNIV
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Problems solved by technology

However, due to the low content of cardiac biomarkers in actual blood samples of patients, especially patients with early cardiovascular and cerebrovascular diseases, and the complex components of actual blood samples, it is difficult for these detection methods to meet the requirements of highly sensitive and selective detection of markers. Its detection sensitivity is low and its selectivity is poor

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  • Preparation method of ferrocene-based covalent organic framework modified electrode and method for electrochemical detection of troponin
  • Preparation method of ferrocene-based covalent organic framework modified electrode and method for electrochemical detection of troponin
  • Preparation method of ferrocene-based covalent organic framework modified electrode and method for electrochemical detection of troponin

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[0030] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] The technical solution of the present invention will be further described below through specific embodiments, and the electrochemical experiment is carried out at a CHI650C electrochemical workstation (Shanghai Chenhua Instrument Co., Ltd.).

[0032] Preparation of ferrocene-based covalent organic framework modified electrodes:

[0033] A) Design a Tro4 single-stranded probe according to the claims, which can specifically bind to target troponin, and the Tro4 single-stranded probe probe sequence of the Tro4 single-stranded probe is: 5'-CGTG CAGT ACGC CAAC CTTT CTCA TGCGCTGCCCCTCTTA-3';

[0034] B) Gold electrode pretreatment: Polish the gold electrode with aluminum powder with a particle size of 1.0 μm, 0.3 μm and 0.05 μm in sequence, and then place the gold electrode in deionized water, ethanol and ethanol solution with a volume rati...

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Abstract

Preparation method of ferrocene-based covalent organic framework modified electrode and method for electrochemically detecting troponin , electrochemistry and computer technology, have achieved good results in the analysis and evaluation indicators such as detection limit. It shows the incomparable advantages of other methods in the rapid, accurate and efficient analysis of cardiac markers and the development of equipment miniaturization. At the same time, the electrochemical aptamer sensor has the advantages of fast detection speed, high sensitivity, strong specificity, simplicity and portability. Due to its relatively low cost-effectiveness and miniaturization, it has shown a good application prospect in the field of troponin detection.

Description

technical field [0001] The invention relates to the field of electrochemical sensing, in particular to a method for preparing a ferrocene-based covalent organic framework modified electrode and a method for electrochemically detecting troponin. Background technique [0002] Cardiac troponin (cTn) is a regulatory protein of muscle tissue contraction, located on the thin myofilament of contractile protein, and plays an important regulatory role in the process of muscle contraction and relaxation. A large number of studies in recent years have shown that troponin is closely related to the occurrence of acute myocardial infarction (AMI), and can be used as a specific biomarker of AMI. Therefore, it is of great significance to establish a rapid, accurate and sensitive troponin detection technology for the early warning and treatment of AMI. [0003] At present, the main cardiac markers (troponin, etc.) detection technologies mainly include dry chemical method, immunoassay (immun...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48G01N33/68
CPCG01N27/3275G01N27/48G01N33/6887
Inventor 高凤汪庆祥宋娟
Owner MINNAN NORMAL UNIV
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