A method for assembling nano-gold cubes to form dimer structures using DNA origami templates based on surface-enhanced Raman effects

A surface-enhanced Raman and nano-gold technology, which is applied in the field of DNA nanometers, can solve the problems of assembly neglect and achieve rich diversity, precise spatial addressability, and enhanced plasmonic effects

Active Publication Date: 2022-03-04
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among various plasmonic metal particles, the assembly of non-spherical anisotropic nanoparticles with angularity has been relatively neglected.

Method used

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  • A method for assembling nano-gold cubes to form dimer structures using DNA origami templates based on surface-enhanced Raman effects
  • A method for assembling nano-gold cubes to form dimer structures using DNA origami templates based on surface-enhanced Raman effects
  • A method for assembling nano-gold cubes to form dimer structures using DNA origami templates based on surface-enhanced Raman effects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of triangular DNA origami with specific sites

[0032] Such as figure 1 Shown: Using phage M13mp18 single-stranded DNA as the backbone strand, it anneals with more than two hundred short scaffold strands and dozens of capture strands to form a triangular DNA origami. Its specific operation is:

[0033] (1) Mix M13mp18 bacteriophage circular single-stranded DNA, unmodified staple strand, and end-modified capture strand (Capture DNA) at a molar ratio of 1:10:10, that is, the added volume is 2.5 μL, 5 μL, 5 μL, then 10 μL 10×TAE-Mg 2+ Buffer (Mg 2+ concentration 12.5 mol / L), add ultrapure water to a final volume of 100 μL, shake well.

[0034] (2) Place the mixed solution in step (1) in a PCR instrument and anneal at a rate of 0.1°C / 10 s from 95°C to 20°C. After the reaction, use a 100 kDa ultrafiltration tube to centrifuge to remove excess staple chains. 4 ℃ for later use.

[0035] Among them, the sequence of the capture strand (Capture DNA) m...

Embodiment 2

[0039] Embodiment 2: Preparation of nano gold cubes modified by thiol DNA

[0040] In a certain buffer environment, thiol DNA single strands were added to modify the surface of the nano-gold cube, and the assembly was completed by adding salt (NaCl) aging to obtain a nano-gold cube covered with thiol DNA. Its specific operation is:

[0041] (1) Take 200 μL of 10 nm gold particles in a 1.5 mL centrifuge tube, centrifuge at 12000 rpm for 15 min, remove 120 μL of supernatant, add 10 μL of thiol DNA and 10 μL of 5×TBE (89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.0) buffer solution, shake to mix.

[0042] (2) Place the sample in step (1) in a mixer, and incubate at 37°C and 300 rpm for 4-6 hours.

[0043] (3) Add 10 μL of 3M NaCl solution to the sample incubated in step (2). Pay attention to adding 4 times, each adding 2.5 μL of 3 M NaCl solution at intervals of 30 minutes. Incubate overnight at 37°C. The purpose of adding salt (NaCl) step by step is to enable the assembly of ...

Embodiment 3

[0045] Example 3: Preparation of three dimer configurations

[0046] Such as figure 1 As shown, the thiol DNA-modified nano gold cube prepared in Example 2 was mixed with the triangular DNA origami prepared in Example 1, annealed and assembled. Its specific operation is:

[0047] The DNA origami and nano-gold cubes modified with sulfhydryl DNA were uniformly mixed in 1×TAE-Mg at a molar ratio of 1:2. 2+ In the buffer, the sample was placed in a PCR machine and annealed for 11 h. The annealing program was to cool from 45 °C to 20 °C at a rate of -0.1 °C / 10 s, and cycled for 4 cycles to completely hybridize the DNA origami and the nano-gold cube. The capture chains at different positions were designed on the surface, and finally assembled to form three kinds of nano-gold cubic dimers: face-face (FTF), face-edge (FTS) and edge-edge (STS).

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Abstract

The invention discloses a method for assembling nano-gold cubes to form a dimer structure based on surface-enhanced Raman effect DNA origami templates. The method specifically includes the preparation of triangular DNA origami with specific sites; Preparation; Preparation of three dimer configurations; Efficient and precise assembly of structures characterized by agarose gel electrophoresis and transmission electron microscopy and surface-enhanced Raman scattering of assembled structures detected by scanning electron microscopy and dark-field microscopy co-localization . This method solves the problems of low assembly repeatability and poor structural stability. The establishment of this method also provides a new idea for the construction of nano-optical materials, which is of great significance to the research of nano-photonics.

Description

technical field [0001] The invention belongs to the field of DNA nanotechnology, and in particular relates to a method for assembling nano-gold cubes to form a dimer structure based on a surface-enhanced Raman effect-based DNA origami template. Background technique [0002] When some molecules are adsorbed on the surface of a specific substance (such as Au, Ag or Cu), the Raman spectrum signal intensity of the molecules will increase significantly. This Raman scattering enhancement phenomenon is called surface-enhanced Raman scattering effect. , in the past decade, surface-enhanced Raman scattering technology has been widely used in many fields such as surface adsorption, electrochemistry, catalytic reaction, biochemical sensor, biomedical detection, trace detection and analysis, etc. Strong light field enhancement and effective light collection effectively solve the problem of low sensitivity of Raman spectroscopy. However, this technique has the problems of poor reproduci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65C12P19/34
CPCG01N21/658C12P19/34
Inventor 汪联辉腾楠晁洁
Owner NANJING UNIV OF POSTS & TELECOMM
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