Method for preparing novel larval and juvenile fish specimens

A production method and technology of larvae, which are applied in the field of making new larvae specimens, can solve the problems of inability to observe the important characteristics of sarcomeres of larvae, difficulty in morphological identification of resource larvae, and inability to carry out accurate identification, so as to prevent The effect of calcium ion loss, stable results, and not easy to deteriorate

Inactive Publication Date: 2019-08-06
中国长江三峡集团有限公司中华鲟研究所
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Problems solved by technology

Among them, after alcohol fixation, the tissues were dehydrated, and the larvae and juveniles were severely deformed, making accurate identification impossible; formalin would cause protein deformation, and important features such as the sarcomeres of larvae and juveniles could not be observed. a certain degree of difficulty

Method used

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  • Method for preparing novel larval and juvenile fish specimens
  • Method for preparing novel larval and juvenile fish specimens

Examples

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Embodiment 1

[0033] (1) Preserve and fix 2 larvae that have been cultured for about 1 week (development to the swim bladder inflation stage), and soak them in 10-15% formalin for 2 days;

[0034] (2) Rinse the fixed fish body specimen with clear water for 12-24 hours, dehydrate in 50% ethanol for 1 day, and then dehydrate in 95% ethanol for 1 day;

[0035] (3) Neutralize with saturated sodium borate for 0.5 days to prevent loss of calcium ions.

[0036] (4) with mass concentration of 3% hydrogen peroxide (H 2 o 2 ) Bleach for 1-2 hours to remove epidermal pigment, and wash in trypsin digestion solution, wherein the volume ratio of trypsin digestion solution is saturated sodium borate:water=7:13;

[0037] (5) The specimens were placed in centrifuge tubes and stored away from light, and centrifuged at room temperature at 4000r / min for 3-6 hours to stain until the bone tissue was stained purple. The mass ratio of the staining solution was potassium hydroxide: alizarin: water =1:0.01:100; ...

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Abstract

The invention relates to a method for preparing novel larval and juvenile fish specimens. The method includes preserving and immobilizing larval and juvenile fish, dehydrating the larval and juvenilefish, putting the dehydrated larval and juvenile fish into a saturated sodium borate solution for neutralization, soaking the larval and juvenile fish in a bleaching solution, cleaning the larval andjuvenile fish in trypsin digestive liquid, then adding a staining solution to stain the larval and juvenile fish until the bone tissue is purple-red, washing the fish with clear water to remove the staining solution on the surface, cleaning the fish in trypsin digestive liquid, replacing the trypsin digestive liquid one time a day until the trypsin digestive liquid is clear, and soaking the fish in gradient transparent liquid to prepare the novel larval and juvenile fish specimens. The staining solution is adopted for bone staining of the staining solution in an early stage so as to recognizesarcomeres of fish in the early stage, thus achieving rapid identification for larval fish.

Description

technical field [0001] The invention relates to the field of research on the early life history of fish, in particular to a preparation method of a novel larvae specimen. Background technique [0002] Early fish resource survey is an important method for fish ecology and conservation biology research, which is based on fish eggs and fry in the early life history stage. The investigation and evaluation of early resources through egg collection can not only understand the change characteristics of the scale and age structure of fish breeding populations, but also grasp the change trend of fish population size and the dynamic law of the community, which is an important factor for the development and utilization of fishery resources and the development and utilization of fish resources. An important basis for the protection of natural resources. The identification of species in the early developmental stages of fish is a meticulous and tedious work, and it is also an important ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 郜星晨姜伟胡亚成黄安阳
Owner 中国长江三峡集团有限公司中华鲟研究所
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