Application of LINC02159 to diagnosis and treatment of pulmonary adenocarcinoma

A lung adenocarcinoma and reagent technology, applied in the field of biomedicine, can solve the problem of lncRNA not being discovered

Inactive Publication Date: 2019-08-06
中国人民解放军西部战区总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although lncRNAs play an important role in the development of lung adenocarcinoma, and some lncRNAs can be used as indicators for the diagnosis and prognosis of lung adenocarcinoma, there are still a large number of lncRNAs that have not been discovered, and further research on the expression and regulation of lncRNAs in lung adenocarcinoma is still needed Mechanisms in Tumor Development

Method used

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  • Application of LINC02159 to diagnosis and treatment of pulmonary adenocarcinoma
  • Application of LINC02159 to diagnosis and treatment of pulmonary adenocarcinoma
  • Application of LINC02159 to diagnosis and treatment of pulmonary adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Screening for Gene Markers Related to Lung Adenocarcinoma

[0060] 1. Sample collection

[0061] Surgical samples of 4 lung adenocarcinoma tissues and paired paracancerous tissues were collected respectively. All patients had not received chemotherapy, radiotherapy, targeted therapy, tumor immunotherapy and other treatments before surgery, and had no other tumor diseases or autoimmune diseases. And severe chronic diseases, all patients gave informed consent, and agreed by the organizational ethics committee.

[0062] 2. Preparation of RNA samples

[0063] Cut the tissue into pieces with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes; then add 200μl chloroform (chloroform), close the tube cap tightly, shake vigorously for 15s, and let stand at room temperature for 10min Finally, centrifuge at 11000rpm for 15min at 4°C; transfer the water sample layer to a new centrifuge tube, a...

Embodiment 2

[0074] Example 2 QPCR sequencing to verify the differential expression of LINC02159 gene

[0075] 1. Large-scale QPCR verification of the differential expression of the LINC02159 gene. According to the sample collection method in Example 1, 33 lung adenocarcinoma tissues and 33 corresponding cancer tissues were selected.

[0076] 2, RNA extraction step is the same as embodiment 1

[0077] 3. QPCR

[0078] 1) Reverse transcription reaction

[0079] The FastQ μant cDNA First Strand Synthesis Kit (Product No.: KR106) was used for reverse transcription of lncRNA, and the reaction system and reaction conditions are shown in Table 1.

[0080] Table 1 Reverse transcription reaction system and reaction conditions

[0081]

[0082] 2) Primer design

[0083] QPCR amplification primers were designed according to the coding sequences of the LINC02159 gene and the GAPDH gene in Genebank. Among them, the primers were designed for the common regions of different transcripts of LINC02...

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Abstract

The invention discloses an application of LINC02159 to diagnosis and treatment of pulmonary adenocarcinoma. Through high-flux sequencing and bioinformatics analysis, the expression up regulation of the LINC02159 in patients suffering from pulmonary adenocarcinoma is found for the first time, besides, through QPCR, the feasibility of the LINC02159 as a molecular marker to be applied to diagnosis ofthe pulmonary adenocarcinoma is subjected to large sample verification, and a new means is provided for accurate diagnosis and individualized treatment of the pulmonary adenocarcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of LINC02159 in the diagnosis and treatment of lung adenocarcinoma. Background technique [0002] Lung cancer is a worldwide health problem. In China, the morbidity and mortality of lung cancer have ranked first for many years, posing a huge threat to the health and life of the Chinese population and causing a huge social and economic burden. Non-small cell lung cancer accounts for about 85% of the total number of lung cancers. In terms of personalized treatment of non-small cell lung cancer, the current research on non-small cell lung cancer has gone deep into the gene level. Related molecular markers such as epidermal growth factor receptor, tumor The expression level of protein p53 may directly affect clinicians' choice of treatment options. With the refinement of non-small cell lung cancer treatment options and the continuous development of molecular targeted therapy, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/7105A61P35/00A61P11/00
CPCA61K31/7105A61K45/00A61P11/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 徐红玉张涛李华李东高辉苏晓妹程朋张伶
Owner 中国人民解放军西部战区总医院
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