A kind of dsRNA of migratory locust fatty acid elongase gene lmelo and its preparation method and application

A technology of fatty acid elongase and migratory locust, applied in the field of genetic engineering, can solve the problems of pest resistance, reducing control efficiency, destroying ecological environment, non-target biological hazards and the like

Active Publication Date: 2020-09-29
SHANXI UNIV
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Problems solved by technology

However, long-term use of chemical pesticides will not only cause pests to develop resistance and reduce control efficiency, but also damage the ecological environment and cause harm to other non-target organisms

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  • A kind of dsRNA of migratory locust fatty acid elongase gene lmelo and its preparation method and application
  • A kind of dsRNA of migratory locust fatty acid elongase gene lmelo and its preparation method and application
  • A kind of dsRNA of migratory locust fatty acid elongase gene lmelo and its preparation method and application

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Embodiment Construction

[0020] In order to further illustrate the technical solution of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0021] A dsRNA of migratory locust fatty acid elongase gene LmElo, the nucleotide sequence of the dsRNA is SEQ ID NO:2.

[0022] A method for preparing the dsRNA of migratory locust fatty acid elongase gene LmElo, comprising the following steps:

[0023] a, Based on the transcriptome database of migratory locusts, the fatty acid elongase gene of migratory locusts was searched using bioinformatics methods. After sequence analysis, splicing and comparison, the sequence of fatty acid elongase gene LmElo of migratory locusts was obtained, and primerpremier5.0 software was used Design upstream primer sequence and downstream primer sequence, and send to Sangon Bioengineering (Shanghai) Co., Ltd. for synthesis;

[0024] b. Select five-instar migratory locust nymphs that grow healt...

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Abstract

The invention belongs to the technical field of genetic engineering and particularly relates to dsRNA of a migratory locust fatty acid elongase gene LmElo and a preparation method and application of the dsRNA. The preparation method of the dsRNA of the migratory locust fatty acid elongase gene LmElo comprises the following steps of a, designing an upstream primer sequence and a downstream primer sequence and synthesizing the upstream primer sequence and the downstream primer sequence; b, combining with upstream and downstream primers for designing the migratory locust fatty acid elongase geneLmElo, obtaining a full-length fragment of the fatty acid elongase gene through PCR amplification, purifying an obtained product, cloning and converting the product into escherichia coli, performing sequencing, conducting verification and obtaining the full-length nucleotide sequence of the gene, wherein the nucleotide sequence is shown as SEQ ID NO:1; c, designing upstream and downstream primersof the dsRNA on the basis of the sequence of the migratory locust fatty acid elongase gene which is shown as SEQ ID NO:1, wherein the sequences of the upstream and downstream primers are shown as SEQID NO:3 and SEQ ID NO:4 respectively, and the upstream primer and the downstream primer both carry a T7 promoter subsequence; d, carrying out transcriptive synthesis of the dsRNA.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a dsRNA of migratory locust fatty acid elongase gene LmElo and a preparation method and application thereof. Background technique [0002] Migratory locust is a kind of agricultural pest with great harm. It has a wide distribution range, strong migration ability and great destructive power, so it causes serious harm to agriculture. At present, the method commonly used to control migratory locusts still relies on chemical control, that is, chemical insecticides. However, long-term use of chemical pesticides will not only cause pests to develop resistance and reduce control efficiency, but also damage the ecological environment and cause harm to other non-target organisms. Therefore, there is an urgent need to develop new pest control methods. [0003] The RNA interference (RNAi) technology, which won the Nobel Prize in 2006, is a specific post-transcripti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A01N57/16A01P7/04
CPCA01N57/16C12N15/113C12N2310/14C12N2330/50
Inventor 张建珍杨洋赵小明刘卫敏马恩波
Owner SHANXI UNIV
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