31 results about "Fatty acid elongases" patented technology

The invention belongs to the technical field of genetic engineering and particularly relates to dsRNA of a migratory locust fatty acid elongase gene LmElo and a preparation method and application of the dsRNA. The preparation method of the dsRNA of the migratory locust fatty acid elongase gene LmElo comprises the following steps of a, designing an upstream primer sequence and a downstream primer sequence and synthesizing the upstream primer sequence and the downstream primer sequence; b, combining with upstream and downstream primers for designing the migratory locust fatty acid elongase geneLmElo, obtaining a full-length fragment of the fatty acid elongase gene through PCR amplification, purifying an obtained product, cloning and converting the product into escherichia coli, performing sequencing, conducting verification and obtaining the full-length nucleotide sequence of the gene, wherein the nucleotide sequence is shown as SEQ ID NO:1; c, designing upstream and downstream primersof the dsRNA on the basis of the sequence of the migratory locust fatty acid elongase gene which is shown as SEQ ID NO:1, wherein the sequences of the upstream and downstream primers are shown as SEQID NO:3 and SEQ ID NO:4 respectively, and the upstream primer and the downstream primer both carry a T7 promoter subsequence; d, carrying out transcriptive synthesis of the dsRNA.

The invention discloses a method for increasing the palmitoleic acid content of Saccharomyces cerevisiae. The present invention provides recombinant bacteria, in order to carry out the following 1) transformation in the oil-producing Saccharomyces cerevisiae to obtain the recombinant bacteria: 1) the recombinant bacteria obtained by improving the transcriptional activity of the OLE1 gene in the oil-producing Saccharomyces cerevisiae. In the present invention, in the Saccharomyces cerevisiae strain YS58 that produces more oil, the transcription factor Mga2 or the truncated Mga2 gene fragment is overexpressed, the expression level of the OLE1 gene regulating Δ9 desaturase is increased, and the content of palmitoleic acid in Saccharomyces cerevisiae is increased, Simultaneously express the exogenous fatty acid elongase genes KCS and FAE to construct Saccharomyces cerevisiae genetically engineered bacteria and increase the content of palmitoleic acid in Saccharomyces cerevisiae.

Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds or leaves.

Fatty acids, and methods for the production thereof, are provided. Transgenic organisms, microbes, plants, seeds, and cells useful in the production of fatty acids, along with related expression vectors, phages, plasmids, nucleic acids, and enzymes, are also provided. Methods for the production of fatty acids such as docosadienoic acid and docosatrienoic acid, involving the use of winter aconite (Eranthis hyemalis) EhELO1 elongase, are described in detail.