31 results about "Fatty acid elongases" patented technology

The present invention relates to a fungal C_16/18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C_16/18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C_16/18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.

This invention relates to seeds of plant, plants themselves and cells of such plants which comprise a heterologous gene coding for a plant (such as nasturtium (Tropaeolum majus) or Crambe abyssinica) fatty acid elongase (FAE) gene or allelic variant thereof, or combinations of one or both of these FAE genes with an Arabidopsis fatty acid elongase 1 (FAE1) gene, in co-transformation, in reading frame alignment with a promoter capable of increasing expression of said gene(s), when said transformed plant cell is in a seed, said plant cell or seed being capable of producing an increase in proportion of a very long chain monounsaturated or saturated fatty acids when compared with the proportions of said fatty acids in a control plant cell or seed lacking said heterologous FAE gene or genes. The invention also relates to combinations of these fatty acid elongase genes by traditional crossing, sufficient to increase the proportion of very long chain monounsaturated or saturated fatty acids in the seed oil of the progeny compared to the proportion of said fatty acids in either of the parental lines.

The invention belongs to the technical field of genetic engineering and particularly relates to dsRNA of a migratory locust fatty acid elongase gene LmElo and a preparation method and application of the dsRNA. The preparation method of the dsRNA of the migratory locust fatty acid elongase gene LmElo comprises the following steps of a, designing an upstream primer sequence and a downstream primer sequence and synthesizing the upstream primer sequence and the downstream primer sequence; b, combining with upstream and downstream primers for designing the migratory locust fatty acid elongase geneLmElo, obtaining a full-length fragment of the fatty acid elongase gene through PCR amplification, purifying an obtained product, cloning and converting the product into escherichia coli, performing sequencing, conducting verification and obtaining the full-length nucleotide sequence of the gene, wherein the nucleotide sequence is shown as SEQ ID NO:1; c, designing upstream and downstream primersof the dsRNA on the basis of the sequence of the migratory locust fatty acid elongase gene which is shown as SEQ ID NO:1, wherein the sequences of the upstream and downstream primers are shown as SEQID NO:3 and SEQ ID NO:4 respectively, and the upstream primer and the downstream primer both carry a T7 promoter subsequence; d, carrying out transcriptive synthesis of the dsRNA.

The invention provides a Brassica carinata A. braun fatty acid elongase and a coding gene thereof (BcFAE1). The gene is introduced in to yeast; and the erucic acid content in the recombinant yeast reaches 0.27+/-0.24%. The protein and the coding gene thereof have important theoretical and practical significance for the research of a genetic control mechanism of plant erucic acids, the increasement of the erucic acid content and the improvement of related traits, can play an important role in the genetic engineering improvement of the plant erucic acid genes, and have wide application prospects.

This invention relates to nucleic acid sequences coding for a Lunaria annua, Cardamine graeca or Teesdalia nudicaulis fatty acid elongase, yeast cells expressing the genes/enzymes, plants themselves and cells of such plants and seeds which contain a heterologous gene coding for a L. annua, C. graeca or T. nudicaulis fatty acid elongase gene, the plant or seed being capable of producing increased proportion of a very long chain monounsaturated fatty acid, especially nervonic acid and eicosenoic acid, beyond that of a control plant or seed lacking the heterologous FAE gene or genes.

The invention belongs to the field of dairy cattle breeding genetic engineering, and particularly relates to a dairy cattle miRNA gene bta-miRNA29d-3p, and functional verification of the gene shows that after the gene is over-expressed, a key gene of a fatty acid synthesis and metabolism pathway and a key gene of a triglyceride synthesis and metabolism pathway can be inhibited, and meanwhile, the content of triglyceride in cells is reduced; after the gene is interfered, the key gene of the fatty acid synthesis and metabolism pathway and the key gene of the triglyceride synthesis and metabolism pathway can be up-regulated, the content of the triglyceride in the cells is obviously up-regulated, and the bta-miRNA29d-3p directly acts on a fatty acid elongase ELOVL4 gene. Based on such technical effect, a foundation can be laid for regulation of the content of milk fat in the milk of dairy cattle and breeding of high-milk fat varieties.

The invention discloses a method for increasing the content of palmitoleic acid in saccharomyces cerevisiae. The invention provides a recombinant bacterium which is obtained by performing the following transformation in the oil-producing saccharomyces cerevisiae: 1) the recombinant bacterium is obtained by improving the transcriptional activity of an OLE1 gene in the oil-producing saccharomyces cerevisiae. In a saccharomyces cerevisiae strain YS58 which produces more oil, a transcription factor Mga2 or a truncated Mga2 gene segment is overexpressed, and the expression level of an OLE1 gene for regulating delta 9 desaturase is improved, so that the content of the palmitoleic acid in the saccharomyces cerevisiae is improved, exogenous fatty acid elongase genes KCS and FAE are expressed at the same time, a saccharomyces cerevisiae genetically engineered bacterium is constructed, and the content of the palmitoleic acid in the saccharomyces cerevisiae is increased.

The invention provides recombinant yarrowia lipolytica with high oleic acid yield as well as a construction method and application thereof, and belongs to the field of bioengineering. The recombinantyarrowia lipolytica is a yarrowia lipolytica XJ-9 strain, is deposited in the China Center for Type Culture Collection (CCTCC), and has a deposit number of CCTCC NO: M 2020707. According to the yarrowia lipolytica XJ-9 strain, oleate desaturase is knocked out, endogenous acetyl coenzyme A carboxylase, diacylglycerol acyltransferase and fatty acid elongase 1 are overexpressed, heterologous fatty acid elongase 2 and stearoyl coenzyme A desaturase are overexpressed, and experiments prove that the recombinant yarrowia lipolytica can be efficiently fermented to produce oleic acid, and efficient synthesis of plant-derived natural product oleic acid in yarrowia lipolytica is realized.

The invention provides a brassicatournefortii gouan fatty acid elongase and a coding gene thereof (BtFAE1). The gene is introduced in to yeast; and the erucic acid content in the recombinant yeast reaches 2.33+/-0.38%. The protein and the coding gene thereof have important theoretical and practical significance for the research of a genetic control mechanism of plant erucic acids, the increasement of the erucic acid content and the improvement of related traits, can play an important role in the genetic engineering improvement of the plant erucic acid genes, and have wide application prospects.

The invention provides a fatty acid elongase derived from Crambe filiformis and a coding gene thereof CfFAE1. When the gene is introduced into yeast, the recombinant yeast erucic acid content is up to 0.76+/-0.14%. The protein and the coding gene thereof provided by the invention are of great theoretical and practical importance in the research on a plant erucic acid gene regulation mechanism, the increase of plant erucic acid content and the improvement of correlated characters, and can play important roles in the gene engineering improvement of the plant erucic acid gene, thereby having wide application prospects.

The invention discloses a rainbow trout fatty acid elongase gene named as an OmElo gene. The nucleotide sequence of the rainbow trout fatty acid elongase gene is SEQ ID No.1. The OmElo gene is the novel elongase gene obtained after a source gene is optimized and designed according to the preference of encoding gene expression sequential codons in silkworm genome sequential data. The amino acid sequence of the protein encoded by guidance of the OmElo gene is SEQ ID No.2. The invention further provides a recombinant expression vector comprising the OmElo gene and a construction method thereof. A promoter of an expression cassette of the recombinant expression vector is the BmNPV very-early promoter IE1. The recombinant expression vector comprising OmElo gene can be applied to obtaining a silkworm transgenic strain with the high content of cis-11,14,17-epoxyeicosatrienoic acid. The invention further provides a method for improving the content of the cis-11,14,17-epoxyeicosatrienoic acid in silkworm tissue, the rainbow trout fatty acid elongase OmElo gene can be guided into a silkworm embryo, and screening is performed to obtain the target silkworm transgenic strain.

The present disclosure provides methods and compositions for modifying fatty acids in Camelina sativa oil. Fatty Acid Desaturase 2 (FAD2), Fatty Acid Desaturase 3 (FAD3), and/or Fatty Acid Elongase 1 (FAE1) genes regulate fatty acid composition in camelina oil.

The invention provides a thlaspiarvense L. fatty acid elongase and a coding gene thereof (TaFAE1). The gene is introduced in to yeast; and the erucic acid content in the recombinant yeast reaches 0.43+/-0.15%. The protein and the coding gene thereof have important theoretical and practical significance for the research of a genetic control mechanism of plant erucic acids, the increasement of the erucic acid content and the improvement of related traits, can play an important role in the genetic engineering improvement of the plant erucic acid genes, and have wide application prospects.

The invention provides an application of a fatty acid elongase gene in yeast synthesis of nervonic acid. Specifically, the invention provides an engineering bacterium for producing nervonic acid, a gene expression cassette is integrated in a genome of the engineering bacterium, and the gene expression cassette expresses a protein coded by a 3-ketoester acyl-CoA synthase (KCS) gene. The engineering bacterium disclosed by the invention can obviously improve the yield of nervonic acid.

Fatty acids, and methods for the production thereof, are provided. Transgenic organisms, microbes, plants, seeds, and cells useful in the production of fatty acids, along with related expression vectors, phages, plasmids, nucleic acids, and enzymes, are also provided. Methods for the production of fatty acids such as docosadienoic acid and docosatrienoic acid, involving the use of winter aconite (Eranthis hyemalis) EhELO1 elongase, are described in detail.