Scattered light and fluorescent light bimodal flow imaging system

Abstract

The invention provides a scattered light and fluorescent light bimodal flow imaging system. The system comprises a multicolor orthogonal lighting unit, a multichannel microimaging unit and a feeding tube, wherein the multicolor orthogonal lighting unit comprises an excitation light source and a beam shaping module, the multichannel microimaging unit comprises an objective lens, a multispectral image divider and multiple cameras, and the feeding tube is coaxial with the objective lens. The excitation light source can emit exciting light with two or more wavelengths, and the exciting light perpendicularly illuminates the feeding tube at the position overlapping with a focal plane of the objective lens after being shaped by the beam shaping module. The objective lens collects scattered lightand fluorescent light which are emitted by bio-particles, and the multispectral image divider projects the scattered light and the fluorescent light to the different cameras for imaging through a scattering channel and one or more fluorescent light channels according to a specific spectral passband. According to the whole system, the principle is based on a lateral scattered light and fluorescentlight emission microimaging technology of light-sheet lighting, a more accurate plankton observation result can be obtained, and the system can adapt to a wider-spectrum water plankton species range and meet the online measurement demand on non-living particulate matter.

Description

technical field [0001] The invention belongs to the optical instrument technology in the field of biology and environment, and in particular relates to a novel flow imaging system. Background technique [0002] Plankton is the basic component of aquatic ecosystems and plays an important role in the material cycle and energy flow of the entire food chain. Understanding the physiology, ecology, diversity, and processes of plankton is a fundamental requirement for research on marine resources, biodiversity, and ecosystem responses to climate change. However, although the existing plankton observation technology and observation platform have been developed to a certain extent, the detection throughput, specificity and accuracy of the identification and quantification technology are still seriously insufficient, which is the current and future related scientific research and water environment monitoring needs. One of the main challenges faced. [0003] In addition to the vastne...

AI Technical Summary

Problems solved by technology

However, light sheet fluorescence flow imaging technology still has many limitations in solving the problem of plankton observation

First of all, at present, this technology can only rely on the autofluorescence of certain pigments in plankton as an imaging contrast mechanism, so it is difficult to achieve accurate imaging measurements of targets or parts that cannot emit autofluorescence, which limits its application to most zooplankton and zooplankton. Prospects for Bacteria

Secondly, many phytoplankton, especially micro and small eukaryotic cells above 20 μm, usually have transparent structures such as cell walls and flagella without fluorescent pigments. These morphological structures are often unique characteristics of specific phytoplankton species; these characteristics cannot be observed Difficulty not only for subsequent identification but also errors in cell size and / or count measurements

Finally, the development of various molecular techniques can not only fluorescently label plankton without autofluorescent pigments, but also greatly increase its detection specificity; however, the existing light-sheet fluorescence imaging flow cytometry has a single excitation wavelength and spectral imaging Insufficient channels to meet the demand for multispectral imaging detection of more different fluorescent labels in a wider spectral range

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