Rapid field detection kit for sensitivity of tetranychidae type tiny pests and use method

A detection kit and micro-sized technology, which is applied in the field of field rapid detection kits for the sensitivity of spider mite micro-sized pests, can solve the problems of long time consumption, difficulty in obtaining and cultivating pests, and inability to meet the microscope use, etc., to achieve saving The effect of experiment cost

Inactive Publication Date: 2019-08-16
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to obtain and cultivate sensitive strains of most pests, so the method is limited to indoor detection, and it is difficult to apply in the field
[0006] To sum up, the problems existing in the existing technology are: the existing method has high requirements for professional skills, the experiment takes a long time, the collection work is difficult, it is not easy to operate in production practice, and the experimental results cannot be observed in a short period of time , unable to provide a basis for scientific medication in the field in a timely manner
[0007] Difficulties in solving the above technical problems: Grassroots scientific and technical personnel or farmers cannot meet the high requirements for professional skills and operations within a certain period of time, and field experiments cannot meet the use of larger instruments such as microscopes, and traditional collection methods cannot achieve rapid and efficient collection. accurate standard

Method used

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  • Rapid field detection kit for sensitivity of tetranychidae type tiny pests and use method
  • Rapid field detection kit for sensitivity of tetranychidae type tiny pests and use method
  • Rapid field detection kit for sensitivity of tetranychidae type tiny pests and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] 1. The source of the tested insects: a sensitive strain of Tetranychus cinnabarinus, which was obtained from indoor breeding for many years;

[0051] The Yunnan strain of Tetranychus cinnabarinus is collected from a rose garden in Yunnan that has not been sprayed for many years;

[0052] The resistant strain of Tetranychus cinnabarinus was obtained after several generations of indoor chemical screening (100.06 times of cyflumetate, 5.13w times of fenpropathrin);

[0053] They were all placed in the insect culture room of the Institute of Plant Protection, Southwest University, and raised with potted cowpea seedlings, and kept in a light incubator for a long time (temperature 26 ± 1 ° C, relative humidity 60%-80%, light L:D = 14:10h).

[0054] 2. Experimental drugs: fenpropathrin original drug (96%), cyflumetate original drug (97%)

[0055] 3. Experimental design

[0056] The 4-hour sensitive baseline (LC-P line) (LC-P line) of the sensitive strain of Tetranychus cinna...

Embodiment 1

[0089] On June 24, 2018, the sensitivity of Tetranychus cinnabarinus on cowpea in the Bishan District Experimental Base of Chongqing was detected with a field rapid detection kit, and the insects to be tested were inhaled into the drug film tube. The mortality rate was 97.2%. The test results showed that the Tetranychus cinnabarinus in this site is very sensitive to cyflumet, and the regular dose of cyflufen can be continued to control the Tetranychus cinnabarinus in this test base.

[0090] Bishan District has a large area of ​​citrus orchards, with a high level of control and a large amount of pesticides. However, the implementation site has not used cyflufenid to control Tetranychus cinnabarinus for two consecutive years. Sensitive, you can continue to use regular doses of cyflufen to control Tetranychus cinnabarinus in this test base.

Embodiment 2

[0092] On July 15, 2018, the sensitivity of Tetranychus cinnabarinus in the vegetable garden of Shizhu County, Chongqing City was tested with a field rapid detection kit. The mortality rate was 92.3%. The test results showed that the yellow apple aphid in this area was sensitive to fenpropathrin, and regular doses of fenpropathrin could be used to control Tetranychus cinnabarinus in this test base. In fact, because the overall dosage of pesticides in the test base was very low in 2018, Tetranychus cinnabarinus has a high sensitivity to various pesticides. The current situation of pesticide application in the test field in the same year has verified the accuracy and practicability of the diagnostic kit of the present invention.

[0093] The investigation at the implementation site found that the detection results of the diagnostic kit of this embodiment coincided with the frequency and intensity of the drug administration at the detection site, further verifying the accuracy an...

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Abstract

The invention belongs to the technical field of devices for eliminating harmful animals or harmful plants and discloses a rapid field detection kit for sensitivity of tetranychidae type tiny pests anda use method. 4-hour sensitive baseline (LC-P line) of a tetranychus cinnabarinus sensitive strain to fenpropathrin and cyflumetofen is obtained through bioassay with an indoor drug membrane method,the diagnostic dose (LC99) is obtained through calculation. The FRC (field recommendation concentration) of two miticides is searched; two agents are prepared, and diagnostic drug bottles with three concentrations including LC99, 2*LC99 and FRC are made for each agent; about 30 tetranychus cinnabarinus is picked, and the death rate condition is detected after 4 hours. Four tetranychus cinnabarinuspopulations are fed in an outdoor net house, each population is treated with the field recommendation dose, and field control effects are investigated after the agents are applied for 1 d, 10 d and 15 d. The kit agent use concentration is determined and detected according to the correlation between the diagnostic death rate in the drug bottles and the field control effects, and kit manufacture iscompleted.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of pest susceptibility, and in particular relates to a field rapid detection kit for the sensitivity of tiny spider mite pests and a use method. Background technique [0002] Currently, the closest prior art: [0003] Cinnabar spider mite (Tetranychus cinnabarinus) (Boisduval) belongs to Acaridae, Tetranychidae, also known as cotton red spider mite, red spider mite. It sucks juice from the adult mites on the back of leaves. After the leaves are damaged, the leaves appear grayish white or form thin yellow spots , A layer of thin wire mesh is formed on the stems and leaves during the blooming period, which makes the plant grow poorly, and in severe cases, the whole plant dies. Tetranychus cinnabarinus is the most widely distributed and most serious agricultural herbivorous mite in China. It seriously harms 113 species of plants in 32 families, including cotton, wheat, miscellaneous grains, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01M1/06A01M1/20
CPCA01M1/06A01M1/2011
Inventor 徐志峰何林胡缘刘杰张梦宇
Owner SOUTHWEST UNIVERSITY
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