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A kind of method for identifying the variety of camellia flower color bud change

An identification method and flower color technology, applied in the biological field, can solve problems such as no related reports

Active Publication Date: 2022-05-27
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, molecular marker technology is mainly used in the identification of bud mutation varieties, such as the application of ISSR or SSR in apples, pears and other fruit trees to identify their bud mutations, and the use of flower color and anthocyanin components and contents in petals to identify camellia color bud mutation varieties There are no related reports

Method used

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  • A kind of method for identifying the variety of camellia flower color bud change
  • A kind of method for identifying the variety of camellia flower color bud change
  • A kind of method for identifying the variety of camellia flower color bud change

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Experimental program
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Effect test

Embodiment 1

[0050] 1 material

[0051] The fresh flowers of camellia cultivar 'Hong Furong' and its bud varieties 'Pink Furong' and 'Bai Furong' were collected to determine the flower color. The pink part of the petals of 'Pan Furong' was cut, and the white part of the petals of 'Bai Furong' was cut. Determination of flower color and qualitative and quantitative analysis. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming period of the crown periphery were selected from each plant.

[0052] 2 Color determination

[0053] The color measurement was carried out according to the method described above.

[0054] The color measurement data of camellia ‘Hong Furong’ and its bud varieties are shown in Table 2.

[0055] Table 2 Flower color data of camellia ‘Hong Furong’ and its budding varieties

[0056] CIE L * a * b * color system

suit L *

a *

b *

C*

h

'Red Hibiscus' red 54.99±...

Embodiment 2

[0068] 1 material

[0069] The fresh flowers of camellia cultivar 'Hongchang'ecai' and its budding varieties 'Panchang'ecai' and 'Baichang'ecai' were collected to determine their flower color. Among them, 'Panchang'ecai' was cut from the pink part of the petals, and 'Baichang'ecai' was cut from the petals. Flower color determination and qualitative and quantitative analysis were performed on the white part of the petals. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming period of the crown periphery were selected from each plant.

[0070] 2 Color determination

[0071] The color measurement was carried out according to the method described above.

[0072] The color measurement data of camellia 'Hongchang'ecai' and its budding varieties are shown in Table 5, CIE L * a * b * In the system, from the red 'red chang'e color' to the pink 'pink chang'e color' and the white 'white chang'e color', with the color of the petal...

Embodiment 3

[0083] 1 material

[0084] The fresh flowers of camellia cultivar 'Hongwubao' and its budding varieties 'Fenwubao' and 'Baiwubao' were collected to determine their flower color. The pink part of the petals was cut from 'Fanwubao', and the pink part of the petals of 'Baiwubao' was cut. Flower color determination and qualitative and quantitative analysis were performed on the white part of the petals. For each material, 5 plants with the same growth condition were selected, and 3 flowers in the blooming period of the crown periphery were selected from each plant.

[0085] 2 Color determination

[0086] The color measurement was carried out according to the method described above.

[0087] The color measurement data of camellia ‘Hongwubao’ and its bud varieties are shown in Table 8, CIE L * a * b * In the system, from the red 'Red Wubao' to the pink 'Pink Wubao' and the white 'White Wubao', the L* value gradually increased as the petals became lighter, and the 'White Wuba...

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Abstract

The invention discloses a method for identifying varieties of camellia flower color buds, using high-performance liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry to detect the red petals and pink color of camellia varieties. The composition and content of anthocyanins in the petals of white bud varieties were studied, combined with the analysis of flower color phenotypes, the effective identification of camellia color bud changes was carried out, the relationship between camellia flower color bud changes and anthocyanins was clarified, and the color bud change of camellias was revealed. Formed material basis, thus providing a scientific basis for camellia color bud breeding.

Description

technical field [0001] The invention relates to a method for identifying camellia flower color and bud change by utilizing flower color and anthocyanin components and contents in petals, and belongs to the field of biotechnology. Background technique [0002] Camellia ( Camellia japonica ) varieties bloom in winter and spring, with high ornamental value and strong adaptability, are important woody flowers for landscaping and potted plants, and are widely used at home and abroad. Camellia flower color variation is rich, its original flower color is red, through bud transformation can produce pink, white and multi-color varieties, such as camellia 'Red Furong', 'Hong Chang'e Cai' and 'Hong Wubao' through bud transformation to red. Corresponding pink and white varieties were bred from the flower varieties. Plant anthocyanins mainly include flavonoids, carotenoids and alkaloids. The anthocyanin components of camellia plants are mainly flavonoids, including anthocyanins in red ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02A01G7/00
CPCG01N30/02A01G7/00
Inventor 李辛雷王洁殷恒福范正琪李纪元
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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