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Method and system for studying biological cells

A cell and cell body technology, applied in the field of biological cell research, can solve problems such as low signal-to-noise ratio, small sample size, and slow detection

Active Publication Date: 2019-08-16
卢米科思CA控股有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, all of these techniques suffer from low signal-to-noise ratios, slow detection, small sample sizes, and / or only local and indirect interactions with the cell membrane of the probed cell

Method used

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  • Method and system for studying biological cells
  • Method and system for studying biological cells
  • Method and system for studying biological cells

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Embodiment Construction

[0086] Note that the drawings are schematic and are not necessarily drawn to scale, and details that are not necessary for understanding the present invention may be omitted. Unless otherwise stated, the terms "upward", "downward", "below", "above" The etc. refer to the embodiments indicated in the drawings. In addition, elements that are at least substantially the same or perform at least substantially the same function are represented by the same numbers, which facilitates personalization with letter suffixes.

[0087] figure 1 Is a schematic diagram of an embodiment of the manipulation system 1 according to the concept of the present disclosure, figure 2 Is a cross-sectional view of the sample holder, Figure 2A Yes figure 2 The details of the sample holder are shown in "IIA".

[0088] The system 1 includes a sample container 3, and the sample container 3 includes an accommodating space 5 for accommodating a sample 7, and the sample 7 contains one or more biological cell bod...

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PUM

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Abstract

A method of manipulating and / or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on thesample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Description

Technical field [0001] The present disclosure relates to methods and systems for studying biological cells. Background technique [0002] Biological cells have outer membranes. This membrane is the interface between the cell and its environment. Therefore, the membrane provides a platform for multiple processes involving membrane embedding of biomolecules, allowing the biomolecules to physically contact the binding partners in the extracellular space. [0003] Therefore, a variety of techniques have been developed for studying cellular and subcellular processes through interaction with cell membranes, and some techniques have been designed to quantify cell surface components to obtain specific cell types (for example, with or without invasion). Breast cancer cells). Most technologies require physical contact with cells. However, a variety of force field technologies have recently been developed, but these technologies often require foreign objects to adhere to cells and / or damag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N29/22B01L3/00G01N21/03G01N21/64G01N29/24G01N29/34G01N29/036G01N21/17
CPCG01N15/10G01N21/64G01N29/036G01N2015/1006G01N29/222G01N21/0332B01L2200/0652G01N2021/177G01N2291/02466G01N2291/02475G01N2291/0255G01N2291/0256G01N2291/0257G01N2291/0423G01N2291/0426G01N21/6458B01L3/50273B01L3/502761B01L2400/0436G01N29/2437G01N29/2462G01N29/346G01N29/348G01N2291/014G01N2291/02809B01L2300/163B01L7/00B01L2300/0663B01L2300/1822G01N15/1429
Inventor A·坎德利F·奥斯瓦德G·西特尔
Owner 卢米科思CA控股有限公司
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