Microfluidic chip and analysis system

Abstract

Microfluidic chip and analysis system, the microfluidic chip includes sample injection hole, sample injection chamber, gas outlet, sample enrichment chamber, waste liquid chamber, dilution and lysis chamber, siphon pipe, sample injection chamber flow pipe, lysis chamber flow pipe , gas circulation pipeline, sample output pipeline, reagent distribution pipeline, gas outlet pipeline and PCR amplification chamber; the dilution and lysis chambers are connected to each PCR amplification chamber and the second waste liquid chamber through siphon pipes in sequence; the gas outlet is also connected to a The waste liquid chamber and the position where the waste liquid chamber communicates with the air outlet is farther away from the rotation center than the air outlet. Sample enrichment, lysis, dilution after lysis, equal volume distribution, and multi-chamber PCR amplification can all be realized sequentially, which can realize the molecular diagnosis function without nucleic acid purification. On the one hand, the position of the gas outlet can be adjusted according to the demand, on the other hand By designing the siphon pipeline, the relative relationship between capillary force and centrifugal force is cleverly used, and a regulating valve is formed to control the flow of liquid into the reagent distribution pipeline.

Description

technical field [0001] This application relates to the field of centrifugal microfluidics, in particular to microfluidic chips and analysis systems. Background technique [0002] Microfluidics refers to the manipulation of liquids on the submillimeter scale, where the submillimeter scale is generally several microns to hundreds of microns. Microfluidic technology integrates the functions of the entire laboratory, including sampling, dilution, reaction, separation, detection, etc., on a small chip, so it is also called Lab-on-a-Chip. This chip is generally composed of various liquid reservoirs and interconnected microchannel networks, which can shorten sample processing time and achieve maximum utilization efficiency of reagent consumables through precise control of liquid flow. Microfluidic systems are devices that manipulate liquids on the submillimeter scale. Centrifugal microfluidics belongs to a branch of microfluidics, which refers to driving the flow of liquid by rot...

AI Technical Summary

Problems solved by technology

This laboratory needs to implement sample processing, nucleic acid extraction, and PCR amplification partition operations, and must have a good ventilation system. The cost of laboratory construction is high, and often only large medical institutions have the financial resources to build it.

On the other hand, laboratory operators have to hold certificates to work, which also greatly increases labor costs

At the same time, too much manual intervention will inevitably lead to human error

These issues have greatly raised the barriers to technical adoption of PCR-based molecular diagnostics

[0004] Moreover, in the current molecular diagnostic laboratory mode, multi-sample and multi-test project operations are completed in a centralized experimental site, and the process quality control requirements are high.

Moreover, the current molecular diagnostic laboratory mode is generally a multi-sample single-indicator detection mode, and the detection indicators are limited, so it is impossible to screen single-sample multi-indicator infectious pathogens

In addition, although the advantages of molecular diagnostic technology are obvious, molecular diagnostics are also expensive due to the cumbersome steps, time-consuming process, and the need for professionals to operate, and the construction cost of clinical molecular diagnostic laboratories is generally high.

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