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A kind of bacterium h4c8 that reduces cadmium content in wheat and its application

A wheat and bacteria technology, applied in the direction of bacteria, application, and the restoration of polluted soil, can solve the problems of secondary pollution of soil ecological environment, changes in soil physical and chemical properties, and decline in microbial activity, so as to improve wheat quality and reduce cadmium content. , the effect of reducing the ratio

Active Publication Date: 2021-10-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the commonly used materials for stabilizing / passivating heavy metals mainly include lime, fly ash, organic materials, biochar, various phosphates, metal oxides, etc. At present, some studies have found that these materials often contain a certain amount of heavy metals, and they are added in large quantities for a long time Into the soil will inevitably lead to changes in soil physical and chemical properties, microbial activity decline and soil fertility degradation, causing secondary pollution of the soil ecological environment [Cao Xinde, Wei Xiaoxin, Dai Gelian, Yang Yongliang. Soil heavy metal complex pollution and its chemical passivation restoration Technology Research Progress. Journal of Environmental Engineering, 2011, 5(7): 1441-1453.]

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  • A kind of bacterium h4c8 that reduces cadmium content in wheat and its application
  • A kind of bacterium h4c8 that reduces cadmium content in wheat and its application
  • A kind of bacterium h4c8 that reduces cadmium content in wheat and its application

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Embodiment Construction

[0026] The present invention is described in further detail below:

[0027] 1. Screening and identification of strains

[0028] A kind of bacterial strain H4C8 resistant to heavy metal cadmium of the present invention is obtained from the contaminated soil in Xiangchang Town, Yuexi County, Anqing City, Anhui Province. In a bottle, 160r. min -1After fully shaking for 20 minutes, take 0.5mL of soil suspension and add it to 4.5mL of normal saline, and use the ten-fold gradient dilution method to successively dilute to 10 -2 、10 -3 and 10 -4 , respectively take 0.1ml and spread it on 100mg of cadmium. L -1 LB solid medium (peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar powder 20g, pH 7.0-7.2), cultured at 28°C for 72h, picked a single colony on the plate and streaked it on the LB solid medium After purification, it was stored at -70°C with 40% glycerol for future use. Inoculate the strain in LB liquid medium, 28°C 160r min -1 Shake culture for 24-36 ...

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Abstract

The invention discloses a bacterium H4C8 for reducing cadmium content in wheat and its application. The bacterial strain H4C8 is named as Serratia sp. Date: March 28, 2019, deposit number: CGMCC NO.17453, mainly used in the bacteria H4C8 that reduces the cadmium content in wheat to promote the growth of wheat and / or reduce the absorption of heavy metals in wheat. Bacteria H4C8 (Serratia) of the present invention can absorb the cadmium in the culture solution when growing in high-concentration cadmium-containing culture solution (50, 75, 100mg / L), and H4C8 can be inoculated in the cadmium-containing soil to promote the growth of wheat, Significantly reduce the cadmium content of wheat and the ratio of glutenin / prolamin, and improve the quality of wheat.

Description

technical field [0001] The invention belongs to the technical fields of agricultural technology and heavy metal polluted soil bioremediation, and specifically relates to a bacterium H4C8 for reducing cadmium content in wheat and an application thereof. Background technique [0002] Due to the rapid development of my country's industry and the extensive use of agricultural materials, there are varying degrees of cadmium pollution in my country's farmland soil. At present, the area of ​​cadmium-contaminated soil in my country has reached 2×10 5 km, accounting for 1 / 6 of the total cultivated land area, involving 25 areas in 11 provinces and cities, and some areas are seriously polluted. According to research, the current input of cadmium from various human sources has led to an average annual increase of 4 μg.kg of cadmium in the top layer soil (0-20 cm) of farmland in my country. -1 . [0003] Wheat is one of the important food crops in the world and in my country. Accordin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G22/20A01G7/06B09C1/10C12R1/425
CPCA01G7/06A01G22/20B09C1/10C12N1/205C12R2001/425
Inventor 孙乐妮侯雪婷郭迎雪杨恩东曹媛媛欧阳文凯杨章泽
Owner ANHUI AGRICULTURAL UNIVERSITY