A kind of bacterium h4c8 that reduces cadmium content in wheat and its application
A wheat and bacteria technology, applied in the direction of bacteria, application, and the restoration of polluted soil, can solve the problems of secondary pollution of soil ecological environment, changes in soil physical and chemical properties, and decline in microbial activity, so as to improve wheat quality and reduce cadmium content. , the effect of reducing the ratio
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[0026] The present invention is described in further detail below:
[0027] 1. Screening and identification of strains
[0028] A kind of bacterial strain H4C8 resistant to heavy metal cadmium of the present invention is obtained from the contaminated soil in Xiangchang Town, Yuexi County, Anqing City, Anhui Province. In a bottle, 160r. min -1After fully shaking for 20 minutes, take 0.5mL of soil suspension and add it to 4.5mL of normal saline, and use the ten-fold gradient dilution method to successively dilute to 10 -2 、10 -3 and 10 -4 , respectively take 0.1ml and spread it on 100mg of cadmium. L -1 LB solid medium (peptone 10g, yeast powder 5g, sodium chloride 5g, water 1000mL, agar powder 20g, pH 7.0-7.2), cultured at 28°C for 72h, picked a single colony on the plate and streaked it on the LB solid medium After purification, it was stored at -70°C with 40% glycerol for future use. Inoculate the strain in LB liquid medium, 28°C 160r min -1 Shake culture for 24-36 ...
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