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Cunninghamia lanceolata phosphorus efficient utilization fine breed authentication method capable of applying arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi and identification method, which is applied in the field of identification of high-efficiency phosphorus utilization of Chinese fir, can solve the problems of large error and short simulation test time, achieves fast method, improves identification efficiency and reliability, and reduces metabolic consumption. Effect

Active Publication Date: 2019-08-30
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

However, due to the heterogeneity of soil environmental factors in the field, or the short duration of the indoor pot plant simulation test, the current evaluation results may have large errors, and long-term verification is still required before it can be popularized.

Method used

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  • Cunninghamia lanceolata phosphorus efficient utilization fine breed authentication method capable of applying arbuscular mycorrhizal fungi
  • Cunninghamia lanceolata phosphorus efficient utilization fine breed authentication method capable of applying arbuscular mycorrhizal fungi
  • Cunninghamia lanceolata phosphorus efficient utilization fine breed authentication method capable of applying arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Test location: in the greenhouse of Science and Technology Park of Fujian Agriculture and Forestry University.

[0037] Material preparation:

[0038] Pot planting device: A polyethylene material device with a pot mouth diameter of 20 cm, a pot bottom diameter of 16 cm, and a height of 20 cm was used for indoor pot experiments. 4 After soaking in the solution for 20 minutes, take it out, rinse it with clean water, dry it, and set it aside.

[0039]Participating clones of Chinese fir seedlings: No. 25 and No. 36 Chinese fir germplasm materials cultivated by the Chinese fir engineering technology research center of the State Forestry and Grassland Bureau were selected as the research objects. The test materials are annual seedlings, which grow vigorously. The average seedling height at the beginning of the test is 17.27±1.72 cm, and the average diameter is 2.85±0.52 mm. Before the Chinese fir seedlings were potted, the surface of the root system was fully disinfected wi...

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Abstract

The invention discloses a cunninghamia lanceolata phosphorus efficient utilization fine breed authentication method capable of applying arbuscular mycorrhizal fungi, and belongs to the technical fieldof the vegetative propagation of cunninghamia lanceolata. By use of the method, the indoor simulation of a low phosphorus stress condition is observed, and a cunninghamia lanceolate clone of which the root system cortical tissue cell is obviously dissolved and is infected and colonized by a arbuscular mycorrhizal fungus propagule is screened, i.e., the phosphorus efficient utilization fine breedis obtained. By use of the method, the cunninghamia lanceolata fine clone which efficiently utilizes soil phosphorus can be quickly and accurately screened, and the method is suitable for applying tocunninghamia lanceolata high density plantation standing forests.

Description

technical field [0001] The invention belongs to the technical field of Chinese fir asexual reproduction, and in particular relates to an identification method for Chinese fir high-efficiency phosphorus-utilizing varieties using arbuscular mycorrhizal fungi. Background technique [0002] Chinese fir( Cunninghamia lanceolata (Lamb.) Hook) is an excellent tree species for afforestation in the subtropical region of southern my country. Due to the serious phosphorus solidification and low mobility in red soil, the soil available phosphorus that trees can absorb and utilize is extremely scarce. Plants have evolved multiple mechanisms to adapt to low phosphorus stress in this environment, including mycorrhizal symbionts, root elongation and proliferation, morphophysiology, and changes in root architecture. It can be seen that if the genetic potential of plants can be fully tapped and the genotypes with high utilization efficiency of limited soil phosphorus resources can be screen...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/30G01N1/28G01N1/36
CPCG01N1/286G01N1/30G01N1/36G01N21/84G01N2001/2873G01N2021/8466
Inventor 吴鹏飞许静静吴文景邹显花马祥庆
Owner FUJIAN AGRI & FORESTRY UNIV
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