Gene for controlling tomato fruit shape and cloning method and application thereof
A cloning method and tomato technology, applied in the field of plants, can solve the problems that the genes of round, oblate and oval fruits have not been reported, and the regulatory genes of oblong fruits are poorly understood, etc. The effect of fruit demand
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[0038] The present invention is described with examples below, which are only used to explain the present invention, and are not intended to limit the scope of the present invention.
[0039] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention.
[0040] This embodiment provides a method for cloning a gene controlling tomato fruit shape, comprising the steps of:
[0041] S1, group building:
[0042] The female parent LA0963 (S.lycopersicum, mel mutant, quoted from the American Tomato Genetic Resource Center, the fruit phenotype is oval or square, such as figure 2 As shown in the c figure), the male parent LA1589 (S.pimpinellifolium, wild type, the fruit phenotype is a round fruit, as shown in figure 2 (shown in a) hybridization to get tomato LA0963×LA1589F 1 plant, its fruit shape is round, such as figure 2 Shown in Figure b.
[0043] Tomato...
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