A kind of antigen for detecting invasive candidiasis albicans and use thereof

A kind of Candida albicans, invasive technology, applied in the field of biomedicine, can solve the difficult and accurate diagnosis of invasive Candida albicans infection and other problems

Active Publication Date: 2022-04-12
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide an antigen for detecting invasive candidiasis and its application, and the antigen for detecting invasive candidiasis and its application will solve the extremely difficult problem in the prior art. Technical Issues in Accurate Diagnosis of Invasive Candida Albicans Infection

Method used

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  • A kind of antigen for detecting invasive candidiasis albicans and use thereof
  • A kind of antigen for detecting invasive candidiasis albicans and use thereof
  • A kind of antigen for detecting invasive candidiasis albicans and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 sequence selection

[0015] Using NCBI ( http: / / www.ncbi.nlm.nih.gov ) integrated protein search function, using "HWP1" as the search field, searched for the amino acid sequence of hyphal wall protein 1 (Candida albicans), excluded interference items, and selected HWP1 with GenBank accession No. AAC96368 as the analysis sequence (the full length of the sequence is 634aa), The sequence is shown as SEQ ID NO.1:

[0016] Using the NCBI amino acid sequence BLAST function, the selected sequences were compared online.

[0017] Such as figure 1 Sequence comparison results show that: the query sequence has 100% homology with the complete sequence included in NCBI, and also has 100% homology with the included non-full-length sequences within their respective lengths, indicating that the selected HWP1 protein primary structure amino acid Sequences are representative and sequences are available.

Embodiment 2B

[0018] Example 2 B cell epitope prediction

[0019] Comprehensive comparison of multiple antigen prediction analysis software [Godkin A J, Smith KJ, Willis A, et al. Naturally processed HLA class II peptides reveal highly conserved immunogenic flanking region sequence preferences that reflect antinprocessing rather than peptide-MHC interactions. J Immumol, 2001, 166( 11):6720-6727][Nielsen M, Lund O.NN-align-A neural network-based alignment algorithm for MHC class II peptide binding prediction.BMC Bioinformatics.2009,18;10:296.][Nielsen M,Lundegaard C , Lund O. Prediction of MHC class II binding affinity using SMM-align, a novel stabilization matrix alignment method. BMC Bioinformatics. 2007, 8:238.].

[0020] B cell epitope prediction adopts web server PREDICTED ANTIGENIC PEPTIDES, Bcepred( http: / / www.imtech.res.in / raghava / bcipep ), the parameters are set as follows: Immunogenicity="Immunodominant"; Mesure of Neutralization="YES"; Pathogen Group="Fungi" /

[0021] After a c...

Embodiment 3

[0024]Example 3 Th cell epitope prediction

[0025] MHC class II molecule Th antigen epitope prediction adopts the network server NetMHCII( http: / / www.cbs.dtu.dk / services / NetMHCII ) for online analysis [Saha.S, Raghava GP.BcePred: Prediction of Continuous B-Cell Epitopes in Antigenic Sequences Using recurrent neural network. Proteins. 2006, 65(1): 40-48.].

[0026] Select six epitopes HLA-DRB1*0101, HLA-DRB1*0301, HLA-DRB1*0401, HLA-DRB1* on the human leukocyte antigen (human leukocyte antigen, HLA) DR loci, which are widely representative 0701, HLA-DRB1*1101, and HLA-DRB1*1501 are used as binding sites, and every 15 amino acid residues are used as walking units to submit query tasks, download the Ecexl worksheet, analyze and verify that it may become a wide range of MHC II antigens HWP1 amino acid sequence of the epitope. Diagram of parameter settings for online prediction of Th cell epitope figure 2 , image 3 shown.

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Abstract

The present invention provides an antigen for detecting invasive candidiasis, the amino acid sequence of which is shown in SEQ ID NO.4. The present invention also provides an antibody against the above-mentioned antigen for detecting invasive candidiasis. The present invention provides the use of the above antigen in the preparation of a reagent for detecting invasive candidiasis albicans. Validated in clinically diagnosed patients, the sensitivity of mimetic peptide a was 70.59%; the specificity was 85%. The antigens of the present invention can therefore be used for early detection of invasive candidiasis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an antigen and its application, in particular to an antigen for detecting invasive candidiasis albicans and its application. Background technique [0002] In recent years, with the widespread use of corticosteroids, immunosuppressants and antibiotics, invasive fungal disease (IFD) has shown a rising trend, and the incidence of infection is getting higher and higher, ranking among the highest infection rates in immunosuppressed patients. First place [Nielsen M, Lundegaard C, Blicher T, Peters B, Sette A, Justesen S, Buus S, Lund O. Quantitative predictions of peptide binding to any HLA-DR molecule of known sequence: NetMHCIIpan.PLoS Comput Biol, 2008, 4(7):1000-1107.] [Singh H, Raghava GP. ProPred: prediction of HLA-DR binding sites. Bioninformatics, 2001, 17(12): 1236-1237.], in IFD, yeast infection accounts for the absolute Most of them, and Candida albicans is the most common yeast inf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/40C07K16/14G01N33/569
CPCC07K14/40C07K16/14G01N33/56961
Inventor 吴学玲孙晓远
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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