Microscope focusing method, device, terminal equipment and storage medium

Abstract

The present invention is applicable to the field of microscopic imaging technology, and provides a focusing method, device, terminal equipment and storage medium for a microscope. The method includes: controlling the stage to move along the direction or Move in a direction away from the objective lens, collect a set of first images of the sample on the stage during the movement of the stage, and record the position information of the stage when collecting each first image; acquire the position information of each first image Sharpness value, and according to the sharpness value and position information of each first image, generate the first focus curve; determine whether the position information corresponding to the peak of the first focus curve is within the maximum or minimum value of the first displacement range. Set the offset within; if not, control the stage to move to the position represented by the position information corresponding to the peak. It solves the problem that the focusing of the fluorescence microscope takes a lot of time and reduces the imaging quality, realizes shortening the focusing time, and ensures the quality of microscopic imaging.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to a microscopic focusing method, device, terminal equipment and storage medium. Background technique [0002] Fluorescence microscopy imaging has important applications in the field of biomedical research, and the autofocus method of fluorescence microscopy is the key to fluorescence microscopy imaging. In order to ensure the successful focusing of the fluorescence microscope, the existing autofocus method needs to ensure that the stage must pass through the focal point when the in-focus image is collected. In order to ensure that the stage passes through the focal point, it is necessary to set a larger movement range of the stage, but it takes a lot of time to focus the fluorescence microscope, and the fluorescent sample will be quenched with time, which will eventually reduce the imaging quality . Contents of the invention [0003] In view of this, the...

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Problems solved by technology

[0003] In view of this, the embodiment of the present invention provides a microscope focusing method, device, terminal equipment and storage medium to solve the problem in the prior art that the focusing of a fluorescence microscope takes a lot of time and reduces the imaging quality

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