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Cystic echinococcosis diagnostic kit and application thereof

A diagnostic kit and kit technology, applied in biological testing, material testing products, measuring devices, etc., can solve problems such as protein insolubility, and achieve the effects of high sensitivity, strong specificity, and simple operation.

Active Publication Date: 2019-09-20
CHINA ANIMAL DISEASE CONTROL CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Jiang Li et al. used the pET32a vector, pET28a vector and pGEX4T-1 vector to express the AgB1 and AgB2 subunit genes cloned from the isolates of Echinococcus granulosus (Xinjiang source and Gansu source) in China. The results showed that AgB1 could not be expressed in the pET28a vector. Expressed, but can be expressed in pGEX4T-1 and pET32a, but the expressed proteins are all insoluble

Method used

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  • Cystic echinococcosis diagnostic kit and application thereof
  • Cystic echinococcosis diagnostic kit and application thereof
  • Cystic echinococcosis diagnostic kit and application thereof

Examples

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Embodiment 1

[0042] Example 1. Time-resolved fluorescent immunoassay (TRFIA) detection of antibodies against Echinococcus granulosus infection

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Abstract

The invention discloses a cystic echinococcosis diagnostic kit and application thereof. The kit comprises a coating antigen composed of an eB2-3 conjugate and / or an eB3-3 conjugate; the eB2-3 conjugate represents a complete antigen obtained by coupling of eB2-3 with carrier protein; the eB3-3 conjugate represents a complete antigen obtained by coupling of eB2-3 with carrier protein; eB2-3 represents polypeptide shown by the sequence 1; and eVP1-2 represents polypeptide shown by the sequence 2. The kit is high in specificity, sensitivity and accuracy, simple and rapid in operation, and suitable for rapidly screening and detecting a lot of serum antibody infected by echinococcus granulosus by veterinary departments and entry-exit inspection and quarantine bureaus in all levels.

Description

technical field [0001] The invention relates to a diagnostic kit for cystic echinococcosis and its application. Background technique [0002] Hydatid disease is the abbreviation of echinococcosis, which is a zoonotic parasitic disease caused by the larvae of Echinococcus parasitic in humans or cattle and sheep. Echinococcosis is mainly transmitted by canine animals such as dogs and foxes, and is divided into granular echinococcosis, multilocular echinococcosis, oligoarticular echinococcosis and echinococcosis fowleri. Cystic echinococcosis (echinococcosis granulosus) caused by larvae of Echinococcus granulosus and alveolar echinococcosis (multilocular echinococcosis) caused by larvae of Echinococcus granulosus mainly exist in my country. Atrial echinococcosis is a serious hazard and has a high fatality rate, also known as "worm cancer". [0003] Echinococcus granulosus is also known as Echinococcus granulosus. Adults parasitize canine carnivores, while larvae (echinococcus...

Claims

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Application Information

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IPC IPC(8): G01N33/58
CPCG01N33/581G01N33/582G01N2333/43543
Inventor 孙雨王传彬宋晓晖肖颖赵晓春杨林蒋菲王睿男曹丽萍央珍王美君白崇生李硕李晓霞刘林青曾邦权王文肖开提·阿不都克里木邹联斌韦正吉刘健鹏林汉亮扎西卓玛徐琦苏晓慧刘玉良毕一鸣马英亢文华秦菊薛文马晓燕任娟李舵杨天意孙航
Owner CHINA ANIMAL DISEASE CONTROL CENT
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