Application of rhizopus niger extracellular polysaccharide to preparation of medicines for treating induced colon cancer

Abstract

The invention provides an application of rhizopus niger extracellular polysaccharide to preparation of medicines for treating induced colon cancer. The rhizopus niger extracellular polysaccharide is obtained through further separation and purification on rhizopus nigricans of activating, fermentation culture, ethanol precipitation, deproteinization by a Sevage method, decolourization with macroporous resin and the like. The anti-tumor activity of the rhizopus niger extracellular polysaccharide is explored by establishing an AOM / DSS induced colon cancer mouse model and a DMH induced bandicoot colon cancer model through the rhizopus niger extracellular polysaccharide. The animal model experiment result indicates that the rhizopus niger extracellular polysaccharide can obviously restrain growth and development of tumors of an induced colon cancer mouse, and has significant prevention and treatment effects on the induced colon cancer. Besides, the rhizopus niger extracellular polysaccharide can increase the health state of mice, increase the survival rate of the mice and delay death.

Description

technical field

[0001] The invention relates to the application of Rhizopus niger exopolysaccharide in the preparation of medicines for treating induced colon cancer, and belongs to the technical field of medicine and health food. Background technique

[0002] Carcinoma refers to a malignant tumor originating from epithelial tissue. In recent years, with the improvement of people's living standards and changes in lifestyle, the incidence of cancer has gradually increased. Cancer has become the second leading cause of death in the world. Colon cancer is a malignant tumor of the digestive tract that occurs in the colon and is the most common type of malignant tumor. According to the 2015 Chinese cancer statistics, the incidence and mortality of colorectal cancer in my country rank among the top 5 among all malignant tumors, and are rising rapidly year by year, which has seriously endangered people's health.

[0003] At present, the main method of colon cancer treatment is ch...

Examples

Embodiment 1

[0047] Embodiment 1: the preparation of Rhizopus niger exopolysaccharide

[0048] (1) Preparation of fermentation broth: thaw Rhizopus niger strains stored in a -20°C refrigerator at room temperature and inoculate 5 μL on a PDA medium plate. After activating for 24 hours, use a hole puncher with an inner diameter of 1 cm to punch the edge of the colony The wells were then transferred to PDB medium, and cultured continuously for 10 days at 28° C. and 120 rpm / min to obtain a fermentation broth.

[0049] The components per liter of the PDA medium in the step (1) are as follows: 200 g of potatoes; 20 g of glucose; 15 g of agar; 1 L of distilled water.

[0050] The components per liter of the PDB medium in the step (1) are as follows: 200 g of potatoes; 20 g of glucose; 1 L of distilled water.

[0051] (2) Preparation of crude extract: select the method of suction filtration to remove mycelia and spores, and place the collected fermentation broth in a rotary evaporator for distillat...

Embodiment 2

[0056] Example 2: Effect of prophylactic administration of Rhizopus niger exopolysaccharide on AOM / DSS-induced colon cancer

[0057] experimental method:

[0058] Establishment of AOM / DSS-induced colon cancer model:

[0059] BALB / c male mice, weighing 18±2g / mouse, were randomly divided into 3 groups, 15 in each group.

[0060] Blank control group (Control), without any treatment, has been fed with ordinary drinking water until the end of the three cycles;

[0061] AOM / DSS model group: first fed with the same volume of sterilized normal saline for 7 consecutive days, then intraperitoneally injected 10 mg / KgWt with azomethane (AOM), and then treated with dextran sodium sulfate (DSS) (molecular weight: 36 , 000-50,000; Concentration: 2.5%) solution was drunk continuously for 7 days, followed by continuous feeding with ordinary drinking water for 14 days as a cycle, repeated three cycles, and the mice were killed after resting for 7 days in the last cycle;

[0062] AOM / DSS+EPS1...

Embodiment 3

[0075] Example 3: Effects of Rhizopus niger exopolysaccharide therapeutic administration on body weight and survival rate of AOM / DSS-induced colon cancer

[0076] experimental method:

[0077] BALB / c male mice, weighing 18±2g / mouse, were randomly divided into 3 groups, 20 in each group.

[0078] Blank control group (Control): same as pre-administration

[0079] AOM / DSS model group: Inject 10 mg / KgWt intraperitoneally with azomethane (AOM), and drink continuously with dextran sodium sulfate (DSS) (molecular weight: 36,000-50,000; concentration: 2.5%) solution one week later 7 days, followed by continuous feeding with the same volume of sterilized saline for 14 days as a cycle, repeated three cycles, and the mice were killed after a 7-day rest in the last cycle;

[0080] AOM / DSS+EPS1-1 direct administration group: AOM, DSS treatment scheme is the same as group A; then feed EPS1-1 (180mg / kg) until the end of the third cycle.

[0081] During the 12 weeks of the experiment, obse...

technical field

[0001] The invention relates to the application of Rhizopus niger exopolysaccharide in the preparation of medicines for treating induced colon cancer, and belongs to the technical field of medicine and health food. Background technique

[0002] Carcinoma refers to a malignant tumor originating from epithelial tissue. In recent years, with the improvement of people's living standards and changes in lifestyle, the incidence of cancer has gradually increased. Cancer has become the second leading cause of death in the world. Colon cancer is a malignant tumor of the digestive tract that occurs in the colon and is the most common type of malignant tumor. According to the 2015 Chinese cancer statistics, the incidence and mortality of colorectal cancer in my country rank among the top 5 among all malignant tumors, and are rising rapidly year by year, which has seriously endangered people's health.

[0003] At present, the main method of colon cancer treatment is ch...

Examples

Embodiment 1

[0047] Embodiment 1: the preparation of Rhizopus niger exopolysaccharide

[0048] (1) Preparation of fermentation broth: thaw Rhizopus niger strains stored in a -20°C refrigerator at room temperature and inoculate 5 μL on a PDA medium plate. After activating for 24 hours, use a hole puncher with an inner diameter of 1 cm to punch the edge of the colony The wells were then transferred to PDB medium, and cultured continuously for 10 days at 28° C. and 120 rpm / min to obtain a fermentation broth.

[0049] The components per liter of the PDA medium in the step (1) are as follows: 200 g of potatoes; 20 g of glucose; 15 g of agar; 1 L of distilled water.

[0050] The components per liter of the PDB medium in the step (1) are as follows: 200 g of potatoes; 20 g of glucose; 1 L of distilled water.

[0051] (2) Preparation of crude extract: select the method of suction filtration to remove mycelia and spores, and place the collected fermentation broth in a rotary evaporator for distillat...

Embodiment 2

[0056] Example 2: Effect of prophylactic administration of Rhizopus niger exopolysaccharide on AOM / DSS-induced colon cancer

[0057] experimental method:

[0058] Establishment of AOM / DSS-induced colon cancer model:

[0059] BALB / c male mice, weighing 18±2g / mouse, were randomly divided into 3 groups, 15 in each group.

[0060] Blank control group (Control), without any treatment, has been fed with ordinary drinking water until the end of the three cycles;

[0061] AOM / DSS model group: first fed with the same volume of sterilized normal saline for 7 consecutive days, then intraperitoneally injected 10 mg / KgWt with azomethane (AOM), and then treated with dextran sodium sulfate (DSS) (molecular weight: 36 , 000-50,000; Concentration: 2.5%) solution was drunk continuously for 7 days, followed by continuous feeding with ordinary drinking water for 14 days as a cycle, repeated three cycles, and the mice were killed after resting for 7 days in the last cycle;

[0062] AOM / DSS+EPS1...

Embodiment 3

[0075] Example 3: Effects of Rhizopus niger exopolysaccharide therapeutic administration on body weight and survival rate of AOM / DSS-induced colon cancer

[0076] experimental method:

[0077] BALB / c male mice, weighing 18±2g / mouse, were randomly divided into 3 groups, 20 in each group.

[0078] Blank control group (Control): same as pre-administration

[0079] AOM / DSS model group: Inject 10 mg / KgWt intraperitoneally with azomethane (AOM), and drink continuously with dextran sodium sulfate (DSS) (molecular weight: 36,000-50,000; concentration: 2.5%) solution one week later 7 days, followed by continuous feeding with the same volume of sterilized saline for 14 days as a cycle, repeated three cycles, and the mice were killed after a 7-day rest in the last cycle;

[0080] AOM / DSS+EPS1-1 direct administration group: AOM, DSS treatment scheme is the same as group A; then feed EPS1-1 (180mg / kg) until the end of the third cycle.

[0081] During the 12 weeks of the experiment, obse...

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