Fast-growing eucalyptus leaf extract and its preparation method and anti-HIV application

Abstract

The invention discloses a fast-growing eucalyptus leaf extract, which is obtained by ultrasonically extracting the fast-growing eucalyptus leaves with a methanol aqueous solution with a volume concentration of 90%, and then separating them by a preparative high-efficiency liquid phase. The inventors used the in vitro cell TZM-bl-HIV-IIIB infection system to conduct research on anti-HIV-1 activity, and found that the cytotoxicity of Su Liuhao is very low, but it has obvious inhibitory effect on the replication of HIV-1. Using the TOA (Time of Addition) method to study the inhibitory mechanism of Su Liuhao on the HIV virus, it was found that the mode of action of Su Liuhao in inhibiting the virus is very similar to that of the clinical drug Enfuvirtide, and it may be a virus entry inhibitor. So far, there are few in vivo and in vitro research reports on the components of fast-growing eucalyptus leaves inhibiting HIV-1 replication. The present invention firstly proposes and confirms that the fast-growing eucalyptus leaves extract has good anti-HIV-1 activity, which provides a new method for the development of clinical AIDS drugs. choose.

Description

technical field [0001] The invention belongs to the technical field of fast-growing eucalyptus application and HIV treatment, and in particular relates to a fast-growing eucalyptus leaf extract and its preparation method and anti-HIV application. Background technique [0002] Human immunodeficiency virus (Human immunodeficiency virus, HIV) can cause AIDS (Acquired immunode-ficiency syndrome, AIDS). -1 replicates and reduces the AIDS mortality rate, but it cannot completely clear the HIV-1 virus in the patient's body, and finding new drugs to treat AIDS has become an inevitable trend. Active ingredients from natural products have the advantages of low toxicity, high efficiency and low cost, and are the source of anti-HIV-1 drug screening. my country is rich in medicinal plant resources, and many natural plant extracts have anti-HIV-1 activity, such as plants of the Polygonaceae genus, plants of the genus Sanbo, and plants of the genus Lemonia. The research on the anti-HIV-1...

AI Technical Summary

Problems solved by technology

[0002] Human immunodeficiency virus (Human immunodeficiency virus, HIV) can cause AIDS (Acquired immunode-ficiency syndrome, AIDS). -1 replicates and reduces the AIDS mortality rate, but it cannot completely clear the HIV-1 virus in the patient's body, and finding new drugs to treat AIDS has become an inevitable trend

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