Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Eucalyputs grandis E.urophylla leaf extract as well as preparation method and anti-HIV application thereof

A technology of leaf extract and fast-growing eucalyptus, which is applied in the field of fast-growing eucalyptus and HIV treatment, and can solve problems such as incomplete removal

Inactive Publication Date: 2019-09-27
GUANGXI MEDICAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Human immunodeficiency virus (Human immunodeficiency virus, HIV) can cause AIDS (Acquired immunode-ficiency syndrome, AIDS). -1 replicates and reduces the AIDS mortality rate, but it cannot completely clear the HIV-1 virus in the patient's body, and finding new drugs to treat AIDS has become an inevitable trend

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Eucalyputs grandis E.urophylla leaf extract as well as preparation method and anti-HIV application thereof
  • Eucalyputs grandis E.urophylla leaf extract as well as preparation method and anti-HIV application thereof
  • Eucalyputs grandis E.urophylla leaf extract as well as preparation method and anti-HIV application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] One, the preparation of fast-growing eucalyptus leaf extract

[0026] 1. Source of fast-growing eucalyptus leaves

[0027] The fast-growing eucalyptus leaves were collected from the eucalypt forests in the suburbs of Nanning, Guangxi. The collection time was spring. The leaves in the middle of the fast-growing eucalyptus trees were selected to be 3 years old. The leaf size was controlled to be 8-17cm long and 3-7cm wide.

[0028] 2. Ultrasonic extraction

[0029] Fresh fast-growing eucalyptus leaves are air-dried and pulverized, add methanol aqueous solution with a volume concentration of 90% according to the weight-to-volume ratio of 50:1, ultrasonically extract at 80°C for 60 minutes, and after standing for 2 days, ultrasonically extract again at room temperature for 120 minutes, and then use a vacuum pump to filter The filtrate was collected, and the filtrate was lyophilized in a lyophilizer to obtain a lyophilized powder.

[0030] 3. Preparative high performance l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention dislcoses an eucalyputs grandis E.urophylla leaf extract. The extract is obtained after eucalyputs grandis E.urophylla leaves are subjected to ultrasonic extraction with a methanol aqueous solution with volume concentration of 90% and through preparation type high performance liquid separation. An in-vitro cell TZM-bl-HIV-IIIB infection system is used for research of anti-HIV-1 activity, which finds that the eucalyputs grandis E.urophylla leaf extract has low cytotoxicity but has an obvious inhibition function on replication of HIV-1. A TOA (time of addition) method is adopted to study the inhibition mechanism of the eucalyputs grandis E.urophylla leaf extract for HIV, which finds that the eucalyputs grandis E.urophylla leaf extract has the virus inhibition acting manner very similar to clinical medicine enfuvirtide and is a virus entry inhibitor. So far, in-vivo and in-vitro study reports about HIV-1 duplication inhibition components of eucalyputs grandis E.urophylla leaves are seldom, the invention proposes and verifies that the eucalyputs grandis E.urophylla leaf extract has good anti-HIV-1 activity for the first time, and a new chose is provided for developing drugs for clinic treatment of AIDS (acquired immune deficiency syndrome).

Description

technical field [0001] The invention belongs to the technical field of fast-growing eucalyptus application and HIV treatment, and in particular relates to a fast-growing eucalyptus leaf extract and its preparation method and anti-HIV application. Background technique [0002] Human immunodeficiency virus (Human immunodeficiency virus, HIV) can cause AIDS (Acquired immunode-ficiency syndrome, AIDS). -1 replicates and reduces the AIDS mortality rate, but it cannot completely clear the HIV-1 virus in the patient's body, and finding new drugs to treat AIDS has become an inevitable trend. Active ingredients from natural products have the advantages of low toxicity, high efficiency and low cost, and are the source of anti-HIV-1 drug screening. my country is rich in medicinal plant resources, and many natural plant extracts have anti-HIV-1 activity, such as plants of the Polygonaceae genus, plants of the genus Sanbo, and plants of the genus Lemonia. The research on the anti-HIV-1...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61P31/18A23L33/105A61K127/00
CPCA23V2002/00A61K36/61A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A23L33/105A61P31/18A23V2200/30A23V2250/21
Inventor 臧宁余军周金爱刘欣覃凤翔梁浩叶力吴睿
Owner GUANGXI MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com