Method for extracting high-montmorillonite lignin by using salvia sclarea L., product obtained through method and response surface optimization technology used in method

A technology of salvia miltiorrhiza and hazy lignin, applied in the direction of organic chemistry, etc., can solve problems such as difficulty in examining the interaction between multiple response values ​​and factors, optimization of the extraction process of salvia miltiorrhiza, and inability to examine the interaction of various factors, etc. Achieve the effect of stable and feasible extraction process, optimized reflux extraction process, and easy operation

Active Publication Date: 2019-10-01
HETIAN UYGHUR PHARMA
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Problems solved by technology

[0003] At present, the optimization methods of extraction process are mostly orthogonal design method and uniform design method. The disadvantage of this type of method is that it cannot examine the interaction between various factors, and it is also difficult to examine the interaction between multiple response values ​​and factors. relation
However, there is no relevant report on the optimization of the extraction process of Danshen miltiorrhiza by using response surface methodology.

Method used

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  • Method for extracting high-montmorillonite lignin by using salvia sclarea L., product obtained through method and response surface optimization technology used in method
  • Method for extracting high-montmorillonite lignin by using salvia sclarea L., product obtained through method and response surface optimization technology used in method
  • Method for extracting high-montmorillonite lignin by using salvia sclarea L., product obtained through method and response surface optimization technology used in method

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Experimental program
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preparation example Construction

[0048] 1.2.1 Preparation of the test solution

[0049] The dried Salvia miltiorrhiza was crushed and passed through a No. 4 sieve to obtain the sample powder. Accurately weigh 1.0000g of the sample powder, put it in a 50ml round bottom flask, add an appropriate amount of ethanol solution, and shake well. Heating to reflux for extraction after weighing. After making up the weight, filter to obtain the test solution.

[0050] 1.2.2 Preparation of reference solution

[0051] Accurately weigh 10.0 mg of high-grade lignin, place it in a 5ml volumetric flask, add ethanol to dissolve, shake well, and obtain the reference substance solution with a concentration of 2.0mg / ml.

[0052] 1.2.3 Chromatographic conditions

[0053] The chromatographic conditions for the determination of high hazy lignin content are: chromatographic column BDS Hypersil C18 (250mm×4.6mm, 5 μm), mobile phase: acetonitrile-0.02% potassium dihydrogen phosphate solution (26-74), detection wavelength 289nm, colu...

Embodiment 1

[0068] Embodiment 1 A kind of response surface method optimizes the extraction process of high hazy lignin in Danshen miltiorrhiza, comprising the following steps:

[0069] (1) After drying the sample of Salvia miltiorrhiza for testing, pulverize it, and pass through a No. 4 sieve to obtain the sample powder;

[0070] (2) The extraction process of high smear lignin in the sample powder is carried out pre-experimentation, and the factors affecting the extraction rate of high smear lignin are ethanol aqueous solution concentration, extraction time, liquid-to-solid ratio and extraction times; and according to the single Factor multi-level experiment, screened out the parameter ranges of ethanol aqueous solution concentration, extraction time, liquid-solid ratio and extraction times;

[0071] (2.1) Ethanol aqueous solution volume concentration parameter range selection

[0072] The single factor experiment of volume concentration of ethanol aqueous solution was carried out at 5 l...

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Abstract

The invention relates to a method for extracting high-montmorillonite lignin by using salvia sclarea L., a product obtained through the method and a response surface optimization technology used in the method. When a single-factor experiment is carried out, comprehensive evaluation of the weight of an extract and the content of the main effective component high-montmorillonite lignin of the salviasclarea L. serve as indexes, and influences of factors such as extraction time, extraction frequency, ethanol concentration and liquid-material ratio are inspected. According to the method, the extraction time, the ethanol concentration and the liquid-material ratio are selected, the content of the high-montmorillonite lignin serves as an index, a Box-Benhnken center combination principle is usedfor a three-factor three-level test, the high-montmorillonite lignin content obtained under the extraction conditions is 0.72 mg / ml, and the difference between the content and the content, predictedby a mode, of 0.73 mg / ml is small. It indicates that the high-montmorillonite lignin extraction process is stable and feasible after response surface method optimization, and the method can be popularized in industrial application.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for extracting high smear lignin by using Salvia miltiorrhiza, the obtained high smear lignin product and the response surface optimization process used. Background technique [0002] Luobufu Kebiri Pill is a commonly used national medicine prescription. It has the functions of warming and tonifying the brain and kidney, nourishing the heart and replenishing the essence, and can be used to treat impotence, premature ejaculation, physical weakness and emaciation, depression and neurasthenia. Luobufu Kebiri Pill is the leading product of Hotan Uygur Pharmaceutical Co., Ltd., and has been registered and approved by CFDA. As one of the main raw materials of Luobufu Kebiri Pills, Salvia miltiorrhiza (scientific name: Salvia sclarea L., also known as red Baiheman, Kizili Baiheman), is a plant of the genus Salvia genus Labiatae. Perennial herba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 庞克坚杨新洲万定荣吴志豪
Owner HETIAN UYGHUR PHARMA
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