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Method for measuring relative contents of anti-interference glycosylated hemoglobin homologs

A technology of relative content of glycosylated hemoglobin, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as interference of abnormal LHbA1c content, high HbA1c measurement, instability, etc.

Inactive Publication Date: 2019-10-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under many conditions, HbA0 can be rapidly converted to labile HbA1c (LHbA1c), resulting in significantly higher HbA1c measurements based on equation (3)
For example, normal adults generally contain 1%-2% LHbA1c, but the relative content of HbA1c is overestimated by about 3.5% under the condition of abnormal increase of 5% LHbA1c, causing obvious positive measurement bias, and equation (3) cannot solve the relative content of LHbA1c Interferenced by abnormal hemoglobin or abnormal hemoglobin content, for example, in the presence of 50% HbE, the current LHbA1c determination method underestimates the actual relative LHbA1c content by 50%, causing a large negative measurement bias

Method used

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  • Method for measuring relative contents of anti-interference glycosylated hemoglobin homologs
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  • Method for measuring relative contents of anti-interference glycosylated hemoglobin homologs

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Embodiment 1

[0036] An anti-interference method for determining the relative content of glycosylated hemoglobin homologues, which uses HPLC technology to separate a certain glycosylated hemoglobin to obtain the characteristic peaks of HbA0, HbA1c and LHbA1c, calculates its relative content in total hemoglobin, and then uses the measurement equation (4) and equation (5) Calculate the relative content of HbA1c and LHbA1c respectively. According to the data provided in Figure 2 in the document A Diabetic Case with Hemoglobin J-Meerut and LowHbA1C Levels, the values ​​of HbA1c(%) and LHbA1c(%) calculated by this method are:

[0037]

[0038]

Embodiment 2

[0040] An anti-interference method for determining the relative content of glycosylated hemoglobin homologues, using capillary electrophoresis to separate a certain glycosylated hemoglobin to obtain the characteristic bands of HbA0, HbA1c and LHbA1c, calculate their relative content in total hemoglobin, and then use the measurement equation (4) and Equation (5) calculates the relative content of HbA1c and LHbA1c respectively. According to Figure 2B in the document Evaluation of the Sebia CAPILLARYS 2 FlexPiercing for the Measurement of HbA1c on Venous and Capillary Blood Samples, the values ​​of HbA1c (%) and LHbA1c (%) calculated by this measurement method are respectively:

[0041]

[0042]

Embodiment 3

[0044]An anti-interference method for determining the relative content of glycosylated hemoglobin homologues, using mass spectrometry to separate a certain hemoglobin to obtain the characteristic peaks of HbA0, HbA1c and LHbA1c, and calculate their relative content in total hemoglobin, and then use the measurement equation (4) to calculate Relative content of HbA1c. In the document "Exploring the Application of Mass Spectrometry MRM Method for the Accurate Determination of Glycated Hemoglobin", the mass-to-charge ratio (m / z) of the characteristic peak of HbA0 (LHbA1c is completely converted into HbA0 during the sample pretreatment process) is 348.2→110 and the m / z of the characteristic peak of HbA1c / z is 429.2→245. According to the area of ​​characteristic peaks and the established standard curve, calculate the concentration of HbA0 (including LHbA1c) h and HbA1c, and finally use the equation (4) to calculate the relative content of HbA1c.

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Abstract

The invention relates to a method for measuring relative contents of anti-interference glycosylated hemoglobin homologs, and aims at respectively measuring the relative contents of HbA1c and LHbA1c according to a ratio of [HbA1c] / ([HbA0]+[HbA1c]+[LHbA1c]) to [LHbA1c] / ([HbA0]+[HbA1c]+[LHbA1c]) on the basis of co-separately detecting the hemoglobin homologs HbA0, GbA1c and LHbA1c. Compared with theexisting glycosylated hemoglobin homolog relative content measurement methods, the method for measuring the relative contents of the anti-interference glycosylated hemoglobin homologs is capable of resisting the influences of various abnormal hemoglobin, hemoglobin relative contents and blood glucose abnormal fluctuation, and objectively and correctly reflecting the relative contents of the glycosylated hemoglobin in the blood.

Description

technical field [0001] The invention relates to the field of measurement and measurement of glycated hemoglobin content, in particular to an anti-interference method for measuring relative content of glycated hemoglobin homologues. Background technique [0002] Glycosylated hemoglobin (GHb) is an important form of hemoglobin, mainly including HbA1a, HbA1b, HbA1c and Labile HbA1c (LHbA1c). Among them, the level of HbA1c can reflect an important indicator of the average blood sugar concentration of the human body in the past 125 days, and it is also a key parameter for blood sugar control, while the level of LHbA1c can reflect the average blood sugar concentration of the human body in the past 2-6 hours, which reflects the short-term Important parameters of blood sugar. [0003] At present, the detection techniques of glycosylated hemoglobin mainly include boric acid affinity chromatography, antigen-antibody immunology method, high performance liquid chromatography (HPLC), ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/447G01N27/62G01N33/72
CPCG01N30/02G01N27/447G01N27/62G01N33/723
Inventor 曹成喜田佑吏查根晗刘伟文王宇兴李红根
Owner SHANGHAI JIAO TONG UNIV