Liquorice homogeneous polysaccharide GUP and preparation method and application thereof

A technology of licorice and polysaccharide, applied in the directions of bone diseases, drug combination, etc., can solve the problems of lowering uric acid and the like that have not been seen in licorice polysaccharide, and achieve the effects of safe use, simple preparation process and wide application prospect.

Inactive Publication Date: 2019-10-08
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Liquorice homogeneous polysaccharide GUP and preparation method and application thereof
  • Liquorice homogeneous polysaccharide GUP and preparation method and application thereof
  • Liquorice homogeneous polysaccharide GUP and preparation method and application thereof

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Embodiment 1

[0035]Take the crushed licorice, add 20 times the weight of petroleum ether to degrease, heat to 70°C, reflux extraction once, 3h, filter, and use dregs for later use; add 95% ethanol with 20 times the weight of dregs to decolorize, heat to 80 ℃, reflux extraction once, 3h, filter, and the dregs for later use; add 10 times of single distilled water to the obtained dregs, heat to 95°C, reflux extraction 3 times, 2h each time, to obtain a water extract. Concentrate the water extract under reduced pressure to 10% of the original volume, add 4 times the volume of 95% ethanol to the concentrated solution for precipitation, add water to dissolve the precipitated part, and obtain a supernatant. Add Sevag reagent (n-butanol:chloroform=1:5v / v) to the supernatant according to 4 times the volume, stir the mixture vigorously for 20min, centrifuge at 10000r / min for 25min in a high-speed centrifuge, and the junction of the water layer and the reagent layer will form The gel-like substance i...

Embodiment 2

[0085] Take the crushed licorice, add 15 times the weight of petroleum ether to degrease, heat to 65°C, extract twice, each time for 2 hours, filter, and use the dregs for later use; add 95% ethanol 15 times the weight of the dregs for decolorization, and heat to 75°C, extract twice, each time for 2 hours, filter, and use the dregs for later use; add 15 times of single distilled water to the obtained dregs, heat to 90°C, extract twice, each time for 2.5h, to obtain a water extract. Concentrate the water extract under reduced pressure to 10% of the original volume, add 4 times the volume of 95% ethanol to the concentrated solution for precipitation, add water to dissolve the precipitated part, and obtain a supernatant. Add Sevag reagent (n-butanol:chloroform=1:5v / v) to the supernatant according to 4 times the volume, stir the mixed solvent vigorously for 30min, centrifuge at 8000r / min for 15min in a high-speed centrifuge, and the junction of the water layer and the reagent layer...

Embodiment 3

[0089] Take the crushed licorice, add 10 times the weight of petroleum ether to degrease, heat to 75°C, extract 3 times, each time for 1.5h, filter, and use the dregs for later use; add 95% ethanol 10 times the weight of the dregs to decolorize, heat to 85°C, extract 3 times, each time for 1.5h, filter, and use the dregs for later use; add 20 times of single distilled water to the obtained dregs, heat to 90°C, extract 3 times, each time for 1.5h, to obtain a water extract . Concentrate the water extract under reduced pressure to 10% of the original volume, add 4 times the volume of 95% ethanol to the concentrated solution for precipitation, add water to dissolve the precipitated part, and obtain a supernatant. Add Sevag reagent (n-butanol:chloroform=1:5v / v) to the supernatant according to 4 times the volume, stir the mixed solvent vigorously for 40min, centrifuge at 8000r / min for 10min in a high-speed centrifuge, and the junction of the water layer and the reagent layer will f...

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Abstract

The invention discloses a preparation method and application of liquorice homogeneous polysaccharide GUP. The homogeneous polysaccharide GUP is obtained by water extraction and alcohol precipitation after licorice is degreased and decolorized by petroleum ether and 95% ethanol and purified by an anion exchange chromatography column and a dextran gel chromatography column after protein removal by aSevag method, the total sugar content of the GUP is 98.39%, the protein content is 0.09%, the glucuronic acid content is 1.66%, the molecular weight is 17416Da, the monosaccharide composition is mannose: rhamnose: glucose: galactose: arabinose = 0.75:5.98:213.54:4.76:15. 51, is alpha-type pyranose, and is not provided with a space triple helix structure. The liquorice homogeneous polysaccharide GUP has the good effect for lowering uric acid and inhibiting xanthine oxidase, and has the potential to be used for preparation of anti-hyperuricemia drugs.

Description

technical field [0001] The invention relates to a licorice homogeneous polysaccharide, a preparation method thereof and an application in preparing uric acid-lowering medicines. Background technique [0002] Licorice, as one of the most commonly used Chinese medicines, has the reputation of the king of Chinese herbal medicines. It has anti-viral, anti-ulcer, anti-diabetic, anti-inflammatory, anti-oxidative, anti-malarial, anti-fungal, anti-bacterial and other activities, and has been used for analgesia, Relief in the treatment of respiratory diseases, gastritis, inflammatory diseases, skin diseases and liver diseases. Modern studies have shown that terpenes, flavonoids and polysaccharides are the main active ingredients in licorice. [0003] Glycyrrhiza polysaccharides have been reported to have various physiological activities, such as antitumor effect, antiviral effect, immune regulation, phagocytosis, anti-complement effect, etc. Because of its wide range of physiologic...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P19/06
CPCA61K31/715A61P19/06C08B37/0003C08B37/006
Inventor 徐希明王启龙余江南郭敏
Owner JIANGSU UNIV
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