Methylophaga strain for online monitoring of heavy metals and application thereof
A technology of methylphagocytic bacteria and heavy metals, applied in the field of microorganisms, can solve the problems that luminous bacteria cannot be monitored online, and achieve the effects of low cost, fast response, rapid response and early warning
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[0046] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.
[0047] 1. Strain screening
[0048] On April 17, April 22, and April 27, 2015, we retrieved three sediment samples (numbered as sediment sample 1 and sediment sample 2 respectively) from the mouth of the wandering river in Yantai City, Shandong Province. , sediment sample 3), and then screened to get a strain of Pb 2+ and Hg 2+ All have strong respiratory inhibition response bacteria, the detailed process of screening is as follows:
[0049] (1) Strain enrichment
[0050] Take 1g of sediment sample 1 and place it in an Erlenmeyer flask containing 100mL of NCM liquid medium, culture it in a constant temperature incubator at 30°C with 140rpm shaking for 5 days, then add 1g of sediment sample 2 to it, and continue culturing for 5 days in the same way. Afterwards, 1 g of sediment sample 3 was added thereto, and the culture was continued f...
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