Methylophaga strain for online monitoring of heavy metals and application thereof

A technology of methylphagocytic bacteria and heavy metals, applied in the field of microorganisms, can solve the problems that luminous bacteria cannot be monitored online, and achieve the effects of low cost, fast response, rapid response and early warning

Active Publication Date: 2019-10-08
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, microorganisms, as indicator organisms, have many advantages such as low culture cost, short reproduction cycle, instant response and sensitivity. However, most of the microorganisms currently used for heavy metal toxicity monitoring are luminescent bacteria. Bacteria cannot be monitored online, freeze-dried powder needs to be prepared, and long-term strain recovery is required, which has certain limitations in environmental heavy metal and toxicity monitoring

Method used

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  • Methylophaga strain for online monitoring of heavy metals and application thereof
  • Methylophaga strain for online monitoring of heavy metals and application thereof
  • Methylophaga strain for online monitoring of heavy metals and application thereof

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Embodiment Construction

[0046] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0047] 1. Strain screening

[0048] On April 17, April 22, and April 27, 2015, we retrieved three sediment samples (numbered as sediment sample 1 and sediment sample 2 respectively) from the mouth of the wandering river in Yantai City, Shandong Province. , sediment sample 3), and then screened to get a strain of Pb 2+ and Hg 2+ All have strong respiratory inhibition response bacteria, the detailed process of screening is as follows:

[0049] (1) Strain enrichment

[0050] Take 1g of sediment sample 1 and place it in an Erlenmeyer flask containing 100mL of NCM liquid medium, culture it in a constant temperature incubator at 30°C with 140rpm shaking for 5 days, then add 1g of sediment sample 2 to it, and continue culturing for 5 days in the same way. Afterwards, 1 g of sediment sample 3 was added thereto, and the culture was continued f...

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Abstract

The invention discloses a Methylophaga strain for online monitoring of heavy metals and application thereof, wherein the Methylophaga strain is selected from the estuary of Guangdang river in Yantai,Shandong, and is an obligate aerobic bacterium, aerobic respiration and oxygen consumption of which having good response sensibility to the two heavy metal ions, Pb2+ and Hg2+; Pb2+ and Hg2+ can inhibit strongly the respiratory action of the Methylophaga strain. The Methylophaga strain is collected under CGMCC No. 13058. The Methylophaga strain and the application thereof have the advantages thatquick response and early warning can be achieved for the heavy metal ions, Pb2+ and Hg2+, in drinking water, surface freshwater and sea water by monitoring changes in oxygen consumption rate, responding speed is high, and operating is simple; the Methylophaga strain can be cultured automatically with no need for the supply of mass bacteria lyophilized powder, and the cost is lower; the Methylophaga strain has an excellent application prospect in real-time online monitoring of heavy metals, research and development of warning technical instruments and other aspects.

Description

technical field [0001] The invention relates to a methylphagous bacterium and its application, in particular to a methylphagic bacterium capable of realizing heavy metal on-line monitoring and its application, and belongs to the technical field of microorganisms. Background technique [0002] In more than a century after the second industrial revolution, human industrial and agricultural production capacity has increased rapidly, and human living standards have been greatly improved. At the same time, there are serious environmental pollution problems. Among them, heavy metal pollution It has always been the focus of pollutants in water pollution. [0003] For nearly a century, human heavy metal poisoning incidents caused by eating fish, shrimp, crab shells enriched in heavy metals have been common. The earliest report was a cadmium poisoning incident (bone pain disease) caused by mine wastewater pollution in Japan in the 1930s . Similar incidents followed. In the 1950s, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/02G01N33/18C12R1/01
CPCC12Q1/02C12N1/205C12R2001/01G01N33/1866Y02A20/20
Inventor 陈令新王巧宁付龙文孙西艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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