Methods for extracting exosomes and exosome proteins

Abstract

The invention discloses a method for extracting exosomes and exosome proteins from human body fluid or cell culture fluid. The method for extracting exosomes and exosome proteins provided by the present invention includes: adding extraction materials to the liquid to be extracted containing exosomes to extract exosomes; the extraction materials are metal oxides or modified with the metal oxides The functional material; the metal oxide can double coordinate with the phosphate ion; the liquid to be extracted is the body fluid from the human body, the dilution of the body fluid or the culture product of cells. Experiments have proved that the exosome extraction method of the present invention can achieve efficient extraction of exosomes in human body fluids or cell culture fluids, the extraction time is short, the obtained exosomes are of high purity, and complete exosomes can be obtained by eluting with an eluent. Exosomes, and the extraction materials used can be recycled and reused; in addition, it can be found that the obtained exosome proteins have many types and are more closely related to the function of exosomes.

Description

technical field [0001] The invention relates to a method for extracting exosomes and exosome proteins from human body fluid or cell culture fluid in the field of biotechnology. Background technique [0002] Exosomes are a type of vesicle-like structure secreted by mammalian cells through the process of "endocytosis-efflux", with a diameter of about 30-150nm. Exosomes exist not only in cell culture fluid, but also in body fluids such as blood, urine, amniotic fluid, breast milk, semen, saliva, and pleural effusion. Their components include lipid bilayers and cell-derived proteins and RNAs. , DNA and other substances. Almost all cells can produce exosomes, and exosomes from different cell sources contain not only common components related to cell formation, structure, and material transport, but also specific molecules related to the biological functions of the source cells. Exosomes, as an important way of intercellular communication and delivery of a large number of biolog...

AI Technical Summary

Problems solved by technology

The first four methods are based on the physical parameters such as the unique density and size of exosomes, which lack specificity and cannot meet the requirements of specific extraction and analysis of tumor cell exosomes in actual samples.

In particular, human body fluid samples contain a large number of particles such as low-density lipoproteins and chylomicrons. The particle size and density of these particles are close to those of exosomes. Most methods cannot separate them from exosomes, so that exosomes Interfering with subsequent analysis

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